Overcoming multidrug resistance through inhalable siRNA nanoparticles-decorated porous microparticles based on supercritical fluid technology

Pei-Yao Xu,1,2 Ranjith Kumar Kankala,1–3 Yu-Jing Pan,1,2 Hui Yuan,1,2 Shi-Bin Wang,1–3 Ai-Zheng Chen1–3 1College of Chemical Engineering, Huaqiao University, Xiamen 361021, P. R. China; 2Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen 36102...

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Autores principales: Xu PY, Kankala RK, Pan YJ, Yuan H, Wang SB, Chen AZ
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Publicado: Dove Medical Press 2018
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spelling oai:doaj.org-article:35559d1bfa8e4ab09ede41be35ca8e502021-12-02T08:45:26ZOvercoming multidrug resistance through inhalable siRNA nanoparticles-decorated porous microparticles based on supercritical fluid technology1178-2013https://doaj.org/article/35559d1bfa8e4ab09ede41be35ca8e502018-08-01T00:00:00Zhttps://www.dovepress.com/overcoming-multidrug-resistance-through-inhalable-sirna-nanoparticles--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Pei-Yao Xu,1,2 Ranjith Kumar Kankala,1–3 Yu-Jing Pan,1,2 Hui Yuan,1,2 Shi-Bin Wang,1–3 Ai-Zheng Chen1–3 1College of Chemical Engineering, Huaqiao University, Xiamen 361021, P. R. China; 2Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen 361021, P. R. China; 3Fujian Provincial Key Laboratory of Biochemical Technology (Huaqiao University), Xiamen 361021, P. R. China Background: In recent times, the co-delivery therapeutics have garnered enormous interest from researchers in the treatment of cancers with multidrug resistance (MDR) due to their efficient delivery of multiple agents, which result in synergistic effects and capable of overcoming all the obstacles of MDR in cancer. However, an efficient delivery platform is required for the conveyance of diverse agents that can successfully devastate MDR in cancer. Methods: Initially, short-interfering RNA-loaded chitosan (siRNA-CS) nanoparticles were synthesized using the ionic gelation method. Further, the siRNA-CS nanoparticles and doxorubicin hydrochloride (DOX) were co-loaded in poly-L-lactide porous microparticles (PLLA PMs) (nano-embedded porous microparticles, [NEPMs]) by the supercritical anti-solvent (SAS) process. Results and discussion: The NEPM formulation exhibited an excellent aerodynamic performance and sustained release of DOX, which displayed higher anticancer efficacy in drug-resistant cells (human small cell lung cancer, H69AR cell line) than those treated with either free DOX and DOX-PLLA PMs due to the siRNA from CS nanoparticles silenced the MDR gene to DOX therapy. Conclusion: This eco-friendly process provides a convenient way to fabricate such innovative NEPMs co-loaded with a chemotherapeutic agent and a gene, which can devastate MDR in cancer through the co-delivery system. Keywords: pulmonary delivery, short-interfering RNA, multidrug resistance, doxorubicin, supercritical carbon dioxideXu PYKankala RKPan YJYuan HWang SBChen AZDove Medical PressarticlePulmonary deliveryShort interfering RNAMultidrug resistanceDoxorubicinSupercritical carbon dioxideMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 4685-4698 (2018)
institution DOAJ
collection DOAJ
language EN
topic Pulmonary delivery
Short interfering RNA
Multidrug resistance
Doxorubicin
Supercritical carbon dioxide
Medicine (General)
R5-920
spellingShingle Pulmonary delivery
Short interfering RNA
Multidrug resistance
Doxorubicin
Supercritical carbon dioxide
Medicine (General)
R5-920
Xu PY
Kankala RK
Pan YJ
Yuan H
Wang SB
Chen AZ
Overcoming multidrug resistance through inhalable siRNA nanoparticles-decorated porous microparticles based on supercritical fluid technology
description Pei-Yao Xu,1,2 Ranjith Kumar Kankala,1–3 Yu-Jing Pan,1,2 Hui Yuan,1,2 Shi-Bin Wang,1–3 Ai-Zheng Chen1–3 1College of Chemical Engineering, Huaqiao University, Xiamen 361021, P. R. China; 2Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen 361021, P. R. China; 3Fujian Provincial Key Laboratory of Biochemical Technology (Huaqiao University), Xiamen 361021, P. R. China Background: In recent times, the co-delivery therapeutics have garnered enormous interest from researchers in the treatment of cancers with multidrug resistance (MDR) due to their efficient delivery of multiple agents, which result in synergistic effects and capable of overcoming all the obstacles of MDR in cancer. However, an efficient delivery platform is required for the conveyance of diverse agents that can successfully devastate MDR in cancer. Methods: Initially, short-interfering RNA-loaded chitosan (siRNA-CS) nanoparticles were synthesized using the ionic gelation method. Further, the siRNA-CS nanoparticles and doxorubicin hydrochloride (DOX) were co-loaded in poly-L-lactide porous microparticles (PLLA PMs) (nano-embedded porous microparticles, [NEPMs]) by the supercritical anti-solvent (SAS) process. Results and discussion: The NEPM formulation exhibited an excellent aerodynamic performance and sustained release of DOX, which displayed higher anticancer efficacy in drug-resistant cells (human small cell lung cancer, H69AR cell line) than those treated with either free DOX and DOX-PLLA PMs due to the siRNA from CS nanoparticles silenced the MDR gene to DOX therapy. Conclusion: This eco-friendly process provides a convenient way to fabricate such innovative NEPMs co-loaded with a chemotherapeutic agent and a gene, which can devastate MDR in cancer through the co-delivery system. Keywords: pulmonary delivery, short-interfering RNA, multidrug resistance, doxorubicin, supercritical carbon dioxide
format article
author Xu PY
Kankala RK
Pan YJ
Yuan H
Wang SB
Chen AZ
author_facet Xu PY
Kankala RK
Pan YJ
Yuan H
Wang SB
Chen AZ
author_sort Xu PY
title Overcoming multidrug resistance through inhalable siRNA nanoparticles-decorated porous microparticles based on supercritical fluid technology
title_short Overcoming multidrug resistance through inhalable siRNA nanoparticles-decorated porous microparticles based on supercritical fluid technology
title_full Overcoming multidrug resistance through inhalable siRNA nanoparticles-decorated porous microparticles based on supercritical fluid technology
title_fullStr Overcoming multidrug resistance through inhalable siRNA nanoparticles-decorated porous microparticles based on supercritical fluid technology
title_full_unstemmed Overcoming multidrug resistance through inhalable siRNA nanoparticles-decorated porous microparticles based on supercritical fluid technology
title_sort overcoming multidrug resistance through inhalable sirna nanoparticles-decorated porous microparticles based on supercritical fluid technology
publisher Dove Medical Press
publishDate 2018
url https://doaj.org/article/35559d1bfa8e4ab09ede41be35ca8e50
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