Regulation of shrimp prophenoloxidase activating system by lva-miR-4850 during bacterial infection

Abstract MicroRNAs (miRNAs) suppress gene expression and regulate biological processes. Following small RNA sequencing, shrimp hemocytes miRNAs differentially expressed in response to acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio parahaemolyticus (VPAHPND) were discovered and some...

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Autores principales:	Pakpoom Boonchuen, Phattarunda Jaree, Kulwadee Somboonviwat, Kunlaya Somboonwiwat
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/35592cce20db413f952bf03cab7e69fc
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spelling	oai:doaj.org-article:35592cce20db413f952bf03cab7e69fc2021-12-02T12:11:07ZRegulation of shrimp prophenoloxidase activating system by lva-miR-4850 during bacterial infection10.1038/s41598-021-82881-22045-2322https://doaj.org/article/35592cce20db413f952bf03cab7e69fc2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82881-2https://doaj.org/toc/2045-2322Abstract MicroRNAs (miRNAs) suppress gene expression and regulate biological processes. Following small RNA sequencing, shrimp hemocytes miRNAs differentially expressed in response to acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio parahaemolyticus (VPAHPND) were discovered and some were confirmed by qRT-PCR. VPAHPND-responsive miRNAs were predicted to target several genes in various immune pathways. Among them, lva-miR-4850 is of interest because its predicted target mRNAs are two important genes of the proPO system; proPO2 (PO2) and proPO activating factor 2 (PPAF2). The expression of lva-miR-4850 was significantly decreased after VPAHPND infection, whereas those of the target mRNAs, PO2 and PPAF2, and PO activity were significantly upregulated. Introducing the lva-miR-4850 mimic into VPAHPND-infected shrimps caused a reduction in the PO2 and PPAF2 transcript levels and the PO activity, but significantly increased the number of bacteria in the VPAHPND targeted tissues. This result inferred that lva-miR-4850 plays a crucial role in regulating the proPO system via suppressing expression of PPAF2 and PO2. To fight against VPAHPND infection, shrimp downregulated lva-miR-4850 expression resulted in proPO activation.Pakpoom BoonchuenPhattarunda JareeKulwadee SomboonviwatKunlaya SomboonwiwatNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pakpoom Boonchuen Phattarunda Jaree Kulwadee Somboonviwat Kunlaya Somboonwiwat Regulation of shrimp prophenoloxidase activating system by lva-miR-4850 during bacterial infection
description	Abstract MicroRNAs (miRNAs) suppress gene expression and regulate biological processes. Following small RNA sequencing, shrimp hemocytes miRNAs differentially expressed in response to acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio parahaemolyticus (VPAHPND) were discovered and some were confirmed by qRT-PCR. VPAHPND-responsive miRNAs were predicted to target several genes in various immune pathways. Among them, lva-miR-4850 is of interest because its predicted target mRNAs are two important genes of the proPO system; proPO2 (PO2) and proPO activating factor 2 (PPAF2). The expression of lva-miR-4850 was significantly decreased after VPAHPND infection, whereas those of the target mRNAs, PO2 and PPAF2, and PO activity were significantly upregulated. Introducing the lva-miR-4850 mimic into VPAHPND-infected shrimps caused a reduction in the PO2 and PPAF2 transcript levels and the PO activity, but significantly increased the number of bacteria in the VPAHPND targeted tissues. This result inferred that lva-miR-4850 plays a crucial role in regulating the proPO system via suppressing expression of PPAF2 and PO2. To fight against VPAHPND infection, shrimp downregulated lva-miR-4850 expression resulted in proPO activation.
format	article
author	Pakpoom Boonchuen Phattarunda Jaree Kulwadee Somboonviwat Kunlaya Somboonwiwat
author_facet	Pakpoom Boonchuen Phattarunda Jaree Kulwadee Somboonviwat Kunlaya Somboonwiwat
author_sort	Pakpoom Boonchuen
title	Regulation of shrimp prophenoloxidase activating system by lva-miR-4850 during bacterial infection
title_short	Regulation of shrimp prophenoloxidase activating system by lva-miR-4850 during bacterial infection
title_full	Regulation of shrimp prophenoloxidase activating system by lva-miR-4850 during bacterial infection
title_fullStr	Regulation of shrimp prophenoloxidase activating system by lva-miR-4850 during bacterial infection
title_full_unstemmed	Regulation of shrimp prophenoloxidase activating system by lva-miR-4850 during bacterial infection
title_sort	regulation of shrimp prophenoloxidase activating system by lva-mir-4850 during bacterial infection
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/35592cce20db413f952bf03cab7e69fc
work_keys_str_mv	AT pakpoomboonchuen regulationofshrimpprophenoloxidaseactivatingsystembylvamir4850duringbacterialinfection AT phattarundajaree regulationofshrimpprophenoloxidaseactivatingsystembylvamir4850duringbacterialinfection AT kulwadeesomboonviwat regulationofshrimpprophenoloxidaseactivatingsystembylvamir4850duringbacterialinfection AT kunlayasomboonwiwat regulationofshrimpprophenoloxidaseactivatingsystembylvamir4850duringbacterialinfection
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Regulation of shrimp prophenoloxidase activating system by lva-miR-4850 during bacterial infection

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