A novel obligate intracellular gamma-proteobacterium associated with ixodid ticks, Diplorickettsia massiliensis, Gen. Nov., Sp. Nov.

<h4>Background</h4>Obligate intracellular bacteria of arthropods often exhibit a significant role in either human health or arthropod ecology.<h4>Methodology/principal findings</h4>An obligate intracellular gamma-proteobacterium was isolated from the actively questing hard ti...

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Autores principales:	Oleg Mediannikov, Zuzana Sekeyová, Marie-Laure Birg, Didier Raoult
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Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/355b36d3438a43168178f7ac14248aec
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spelling	oai:doaj.org-article:355b36d3438a43168178f7ac14248aec2021-12-02T20:20:07ZA novel obligate intracellular gamma-proteobacterium associated with ixodid ticks, Diplorickettsia massiliensis, Gen. Nov., Sp. Nov.1932-620310.1371/journal.pone.0011478https://doaj.org/article/355b36d3438a43168178f7ac14248aec2010-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20644718/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Obligate intracellular bacteria of arthropods often exhibit a significant role in either human health or arthropod ecology.<h4>Methodology/principal findings</h4>An obligate intracellular gamma-proteobacterium was isolated from the actively questing hard tick Ixodes ricinus using mammalian and amphibian cell lines. Transmission electron microscopy revealed a unique morphology of the bacterium, including intravacuolar localization of bacteria grouped predominantly in pairs and internal structures composed of electron-dense crystal-like structures and regular multilayer sheath-like structures. The isolate 20B was characterized to determine its taxonomic position using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that this strain belongs to the family Coxiellaceae, order Legionellales of Gamma-proteobacteria, and the closest relatives are different Rickettsiella spp. The level of 16S rRNA gene sequence similarity between strain 20B and other recognized species of the family was below 94.5%. Partial sequences of the rpoB, parC and ftsY genes confirmed the phylogenetic position of the new isolate. The G+C content estimated on the basis of whole genome analysis of strain 20B was 37.88%. On the basis of its phenotypic and genotypic properties, together with phylogenetic distinctiveness, we propose that strain 20B to be classified in the new genus Diplorickettsia as the type strain of a novel species named Diplorickettsia massiliensis sp. nov.<h4>Conclusions/significance</h4>Considering the source of its isolation (hard tick, often biting humans) the role of this bacterium in the pathology of humans, animals and ticks should be further investigated.Oleg MediannikovZuzana SekeyováMarie-Laure BirgDidier RaoultPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 7, p e11478 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Oleg Mediannikov Zuzana Sekeyová Marie-Laure Birg Didier Raoult A novel obligate intracellular gamma-proteobacterium associated with ixodid ticks, Diplorickettsia massiliensis, Gen. Nov., Sp. Nov.
description	<h4>Background</h4>Obligate intracellular bacteria of arthropods often exhibit a significant role in either human health or arthropod ecology.<h4>Methodology/principal findings</h4>An obligate intracellular gamma-proteobacterium was isolated from the actively questing hard tick Ixodes ricinus using mammalian and amphibian cell lines. Transmission electron microscopy revealed a unique morphology of the bacterium, including intravacuolar localization of bacteria grouped predominantly in pairs and internal structures composed of electron-dense crystal-like structures and regular multilayer sheath-like structures. The isolate 20B was characterized to determine its taxonomic position using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that this strain belongs to the family Coxiellaceae, order Legionellales of Gamma-proteobacteria, and the closest relatives are different Rickettsiella spp. The level of 16S rRNA gene sequence similarity between strain 20B and other recognized species of the family was below 94.5%. Partial sequences of the rpoB, parC and ftsY genes confirmed the phylogenetic position of the new isolate. The G+C content estimated on the basis of whole genome analysis of strain 20B was 37.88%. On the basis of its phenotypic and genotypic properties, together with phylogenetic distinctiveness, we propose that strain 20B to be classified in the new genus Diplorickettsia as the type strain of a novel species named Diplorickettsia massiliensis sp. nov.<h4>Conclusions/significance</h4>Considering the source of its isolation (hard tick, often biting humans) the role of this bacterium in the pathology of humans, animals and ticks should be further investigated.
format	article
author	Oleg Mediannikov Zuzana Sekeyová Marie-Laure Birg Didier Raoult
author_facet	Oleg Mediannikov Zuzana Sekeyová Marie-Laure Birg Didier Raoult
author_sort	Oleg Mediannikov
title	A novel obligate intracellular gamma-proteobacterium associated with ixodid ticks, Diplorickettsia massiliensis, Gen. Nov., Sp. Nov.
title_short	A novel obligate intracellular gamma-proteobacterium associated with ixodid ticks, Diplorickettsia massiliensis, Gen. Nov., Sp. Nov.
title_full	A novel obligate intracellular gamma-proteobacterium associated with ixodid ticks, Diplorickettsia massiliensis, Gen. Nov., Sp. Nov.
title_fullStr	A novel obligate intracellular gamma-proteobacterium associated with ixodid ticks, Diplorickettsia massiliensis, Gen. Nov., Sp. Nov.
title_full_unstemmed	A novel obligate intracellular gamma-proteobacterium associated with ixodid ticks, Diplorickettsia massiliensis, Gen. Nov., Sp. Nov.
title_sort	novel obligate intracellular gamma-proteobacterium associated with ixodid ticks, diplorickettsia massiliensis, gen. nov., sp. nov.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/355b36d3438a43168178f7ac14248aec
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A novel obligate intracellular gamma-proteobacterium associated with ixodid ticks, Diplorickettsia massiliensis, Gen. Nov., Sp. Nov.

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