Arsenic leads to autophagy of keratinocytes by increasing aquaporin 3 expression

Abstract Exposure to arsenic, a ubiquitous metalloid on Earth, results in human cancers. Skin cancer is the most common arsenical cancers. Both autophagy and aquaporin pathway are known to promote carcinogenesis. However, the mechanisms by which arsenic regulates aquaporin and autophagy in arsenical...

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Autores principales:	Sebastian Yu, Ling-Hau Li, Chih-Hung Lee, Palaniraja Jeyakannu, Jeh-Jeng Wang, Chien-Hui Hong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3561235f25c34c3895bf36bbd11b4675
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spelling	oai:doaj.org-article:3561235f25c34c3895bf36bbd11b46752021-12-02T19:04:36ZArsenic leads to autophagy of keratinocytes by increasing aquaporin 3 expression10.1038/s41598-021-96822-62045-2322https://doaj.org/article/3561235f25c34c3895bf36bbd11b46752021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96822-6https://doaj.org/toc/2045-2322Abstract Exposure to arsenic, a ubiquitous metalloid on Earth, results in human cancers. Skin cancer is the most common arsenical cancers. Both autophagy and aquaporin pathway are known to promote carcinogenesis. However, the mechanisms by which arsenic regulates aquaporin and autophagy in arsenical skin cancers remain elusive. This study aims to address how arsenic regulates aquaporin-3, the predominant aquaporin in epidermal keratinocytes, and how this process would induce autophagy. Quantitative real-time PCR and immunofluorescence were used to measure the expression of aquaporin 3 in arsenical skin cancers and arsenic-treated keratinocytes. Beclin-1 expression and autophagy were measured. We examined if blocking aquaporin 3 could interfere arsenic-induced autophagy in keratinocytes. Expression of aquaporin 3 is increased in arsenical cancers and in arsenic-treated keratinocytes. Arsenic induced autophagy in primary human keratinocytes. Notably, the arsenic-induced autophagy was inhibited by pretreatment of keratinocytes with aquaporin inhibitors Auphen or AgNO3, or RNA interference against aquaporin 3. The data indicates that the aquaporin 3 is an important cell membrane channel to mediate arsenic uptake and contributes to the arsenic-induced autophagy.Sebastian YuLing-Hau LiChih-Hung LeePalaniraja JeyakannuJeh-Jeng WangChien-Hui HongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sebastian Yu Ling-Hau Li Chih-Hung Lee Palaniraja Jeyakannu Jeh-Jeng Wang Chien-Hui Hong Arsenic leads to autophagy of keratinocytes by increasing aquaporin 3 expression
description	Abstract Exposure to arsenic, a ubiquitous metalloid on Earth, results in human cancers. Skin cancer is the most common arsenical cancers. Both autophagy and aquaporin pathway are known to promote carcinogenesis. However, the mechanisms by which arsenic regulates aquaporin and autophagy in arsenical skin cancers remain elusive. This study aims to address how arsenic regulates aquaporin-3, the predominant aquaporin in epidermal keratinocytes, and how this process would induce autophagy. Quantitative real-time PCR and immunofluorescence were used to measure the expression of aquaporin 3 in arsenical skin cancers and arsenic-treated keratinocytes. Beclin-1 expression and autophagy were measured. We examined if blocking aquaporin 3 could interfere arsenic-induced autophagy in keratinocytes. Expression of aquaporin 3 is increased in arsenical cancers and in arsenic-treated keratinocytes. Arsenic induced autophagy in primary human keratinocytes. Notably, the arsenic-induced autophagy was inhibited by pretreatment of keratinocytes with aquaporin inhibitors Auphen or AgNO3, or RNA interference against aquaporin 3. The data indicates that the aquaporin 3 is an important cell membrane channel to mediate arsenic uptake and contributes to the arsenic-induced autophagy.
format	article
author	Sebastian Yu Ling-Hau Li Chih-Hung Lee Palaniraja Jeyakannu Jeh-Jeng Wang Chien-Hui Hong
author_facet	Sebastian Yu Ling-Hau Li Chih-Hung Lee Palaniraja Jeyakannu Jeh-Jeng Wang Chien-Hui Hong
author_sort	Sebastian Yu
title	Arsenic leads to autophagy of keratinocytes by increasing aquaporin 3 expression
title_short	Arsenic leads to autophagy of keratinocytes by increasing aquaporin 3 expression
title_full	Arsenic leads to autophagy of keratinocytes by increasing aquaporin 3 expression
title_fullStr	Arsenic leads to autophagy of keratinocytes by increasing aquaporin 3 expression
title_full_unstemmed	Arsenic leads to autophagy of keratinocytes by increasing aquaporin 3 expression
title_sort	arsenic leads to autophagy of keratinocytes by increasing aquaporin 3 expression
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3561235f25c34c3895bf36bbd11b4675
work_keys_str_mv	AT sebastianyu arsenicleadstoautophagyofkeratinocytesbyincreasingaquaporin3expression AT linghauli arsenicleadstoautophagyofkeratinocytesbyincreasingaquaporin3expression AT chihhunglee arsenicleadstoautophagyofkeratinocytesbyincreasingaquaporin3expression AT palanirajajeyakannu arsenicleadstoautophagyofkeratinocytesbyincreasingaquaporin3expression AT jehjengwang arsenicleadstoautophagyofkeratinocytesbyincreasingaquaporin3expression AT chienhuihong arsenicleadstoautophagyofkeratinocytesbyincreasingaquaporin3expression
_version_	1718377202093916160

Arsenic leads to autophagy of keratinocytes by increasing aquaporin 3 expression

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