Comparative analysis of Acinetobacters: three genomes for three lifestyles.
Acinetobacter baumannii is the source of numerous nosocomial infections in humans and therefore deserves close attention as multidrug or even pandrug resistant strains are increasingly being identified worldwide. Here we report the comparison of two newly sequenced genomes of A. baumannii. The human...
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2008
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oai:doaj.org-article:356ae5f5beb7457f9d4110cf13c170732021-11-25T06:13:04ZComparative analysis of Acinetobacters: three genomes for three lifestyles.1932-620310.1371/journal.pone.0001805https://doaj.org/article/356ae5f5beb7457f9d4110cf13c170732008-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18350144/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Acinetobacter baumannii is the source of numerous nosocomial infections in humans and therefore deserves close attention as multidrug or even pandrug resistant strains are increasingly being identified worldwide. Here we report the comparison of two newly sequenced genomes of A. baumannii. The human isolate A. baumannii AYE is multidrug resistant whereas strain SDF, which was isolated from body lice, is antibiotic susceptible. As reference for comparison in this analysis, the genome of the soil-living bacterium A. baylyi strain ADP1 was used. The most interesting dissimilarities we observed were that i) whereas strain AYE and A. baylyi genomes harbored very few Insertion Sequence elements which could promote expression of downstream genes, strain SDF sequence contains several hundred of them that have played a crucial role in its genome reduction (gene disruptions and simple DNA loss); ii) strain SDF has low catabolic capacities compared to strain AYE. Interestingly, the latter has even higher catabolic capacities than A. baylyi which has already been reported as a very nutritionally versatile organism. This metabolic performance could explain the persistence of A. baumannii nosocomial strains in environments where nutrients are scarce; iii) several processes known to play a key role during host infection (biofilm formation, iron uptake, quorum sensing, virulence factors) were either different or absent, the best example of which is iron uptake. Indeed, strain AYE and A. baylyi use siderophore-based systems to scavenge iron from the environment whereas strain SDF uses an alternate system similar to the Haem Acquisition System (HAS). Taken together, all these observations suggest that the genome contents of the 3 Acinetobacters compared are partly shaped by life in distinct ecological niches: human (and more largely hospital environment), louse, soil.David VallenetPatrice NordmannValérie BarbeLaurent PoirelSophie MangenotElodie BatailleCarole DossatShahinaz GasAnnett KreimeyerPatricia LenobleSophie OztasJulie PoulainBéatrice SegurensCatherine RobertChantal AbergelJean-Michel ClaverieDidier RaoultClaudine MédigueJean WeissenbachStéphane CruveillerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 3, p e1805 (2008) |
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DOAJ |
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DOAJ |
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| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q David Vallenet Patrice Nordmann Valérie Barbe Laurent Poirel Sophie Mangenot Elodie Bataille Carole Dossat Shahinaz Gas Annett Kreimeyer Patricia Lenoble Sophie Oztas Julie Poulain Béatrice Segurens Catherine Robert Chantal Abergel Jean-Michel Claverie Didier Raoult Claudine Médigue Jean Weissenbach Stéphane Cruveiller Comparative analysis of Acinetobacters: three genomes for three lifestyles. |
| description |
Acinetobacter baumannii is the source of numerous nosocomial infections in humans and therefore deserves close attention as multidrug or even pandrug resistant strains are increasingly being identified worldwide. Here we report the comparison of two newly sequenced genomes of A. baumannii. The human isolate A. baumannii AYE is multidrug resistant whereas strain SDF, which was isolated from body lice, is antibiotic susceptible. As reference for comparison in this analysis, the genome of the soil-living bacterium A. baylyi strain ADP1 was used. The most interesting dissimilarities we observed were that i) whereas strain AYE and A. baylyi genomes harbored very few Insertion Sequence elements which could promote expression of downstream genes, strain SDF sequence contains several hundred of them that have played a crucial role in its genome reduction (gene disruptions and simple DNA loss); ii) strain SDF has low catabolic capacities compared to strain AYE. Interestingly, the latter has even higher catabolic capacities than A. baylyi which has already been reported as a very nutritionally versatile organism. This metabolic performance could explain the persistence of A. baumannii nosocomial strains in environments where nutrients are scarce; iii) several processes known to play a key role during host infection (biofilm formation, iron uptake, quorum sensing, virulence factors) were either different or absent, the best example of which is iron uptake. Indeed, strain AYE and A. baylyi use siderophore-based systems to scavenge iron from the environment whereas strain SDF uses an alternate system similar to the Haem Acquisition System (HAS). Taken together, all these observations suggest that the genome contents of the 3 Acinetobacters compared are partly shaped by life in distinct ecological niches: human (and more largely hospital environment), louse, soil. |
| format |
article |
| author |
David Vallenet Patrice Nordmann Valérie Barbe Laurent Poirel Sophie Mangenot Elodie Bataille Carole Dossat Shahinaz Gas Annett Kreimeyer Patricia Lenoble Sophie Oztas Julie Poulain Béatrice Segurens Catherine Robert Chantal Abergel Jean-Michel Claverie Didier Raoult Claudine Médigue Jean Weissenbach Stéphane Cruveiller |
| author_facet |
David Vallenet Patrice Nordmann Valérie Barbe Laurent Poirel Sophie Mangenot Elodie Bataille Carole Dossat Shahinaz Gas Annett Kreimeyer Patricia Lenoble Sophie Oztas Julie Poulain Béatrice Segurens Catherine Robert Chantal Abergel Jean-Michel Claverie Didier Raoult Claudine Médigue Jean Weissenbach Stéphane Cruveiller |
| author_sort |
David Vallenet |
| title |
Comparative analysis of Acinetobacters: three genomes for three lifestyles. |
| title_short |
Comparative analysis of Acinetobacters: three genomes for three lifestyles. |
| title_full |
Comparative analysis of Acinetobacters: three genomes for three lifestyles. |
| title_fullStr |
Comparative analysis of Acinetobacters: three genomes for three lifestyles. |
| title_full_unstemmed |
Comparative analysis of Acinetobacters: three genomes for three lifestyles. |
| title_sort |
comparative analysis of acinetobacters: three genomes for three lifestyles. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2008 |
| url |
https://doaj.org/article/356ae5f5beb7457f9d4110cf13c17073 |
| work_keys_str_mv |
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