Influence of Nitric Oxide generated through microwave plasma on L6 skeletal muscle cell myogenesis via oxidative signaling pathways

Abstract Myogenic precursors are myoblasts that have a potency to differentiate into muscle fibers on injury and maintain the regenerative power of skeletal muscle. However, the roles of exogenous nitric oxide (NO) in muscle development and myoblast differentiation are largely unknown. Therefore, in...

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Autores principales:	Naresh Kumar, Priyanka Shaw, Han Sup Uhm, Eun Ha Choi, Pankaj Attri
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/357173c200b54f4e881ca044fb7d299c
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spelling	oai:doaj.org-article:357173c200b54f4e881ca044fb7d299c2021-12-02T12:30:38ZInfluence of Nitric Oxide generated through microwave plasma on L6 skeletal muscle cell myogenesis via oxidative signaling pathways10.1038/s41598-017-00154-32045-2322https://doaj.org/article/357173c200b54f4e881ca044fb7d299c2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00154-3https://doaj.org/toc/2045-2322Abstract Myogenic precursors are myoblasts that have a potency to differentiate into muscle fibers on injury and maintain the regenerative power of skeletal muscle. However, the roles of exogenous nitric oxide (NO) in muscle development and myoblast differentiation are largely unknown. Therefore, in this study, we examined the effects of exogenous NO generated by a microwave plasma torch on rat myoblastic L6 cell proliferation and differentiation. We observed that the differentiation of L6 myogenic precursor cells into myotubes was significantly enhanced after NO treatment. The expression of the myogenesis marker proteins and mRNA level, such as myoD, myogenin, and myosin heavy chain (MHC), as well as the cyclic guanosine monophosphate (cGMP) level, were significantly increased after the NO treatment, without creating toxicity. Moreover, we observed that the oxidative stress signaling [extracellular-signal-regulated kinase (Erks), and Adenosine monophosphate-activated protein kinase (AMPK)] phosphorylation was higher in NO treated cells than in the control cells [without NO treatment]. Therefore, these results reveal the exogenous NO role in regulating myoblast differentiation through the oxidative stress signaling pathway. Through this work, we can suggest that exogenous NO can help in cell differentiation and tissue regeneration, which provides new possibilities for plasma medicine.Naresh KumarPriyanka ShawHan Sup UhmEun Ha ChoiPankaj AttriNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Naresh Kumar Priyanka Shaw Han Sup Uhm Eun Ha Choi Pankaj Attri Influence of Nitric Oxide generated through microwave plasma on L6 skeletal muscle cell myogenesis via oxidative signaling pathways
description	Abstract Myogenic precursors are myoblasts that have a potency to differentiate into muscle fibers on injury and maintain the regenerative power of skeletal muscle. However, the roles of exogenous nitric oxide (NO) in muscle development and myoblast differentiation are largely unknown. Therefore, in this study, we examined the effects of exogenous NO generated by a microwave plasma torch on rat myoblastic L6 cell proliferation and differentiation. We observed that the differentiation of L6 myogenic precursor cells into myotubes was significantly enhanced after NO treatment. The expression of the myogenesis marker proteins and mRNA level, such as myoD, myogenin, and myosin heavy chain (MHC), as well as the cyclic guanosine monophosphate (cGMP) level, were significantly increased after the NO treatment, without creating toxicity. Moreover, we observed that the oxidative stress signaling [extracellular-signal-regulated kinase (Erks), and Adenosine monophosphate-activated protein kinase (AMPK)] phosphorylation was higher in NO treated cells than in the control cells [without NO treatment]. Therefore, these results reveal the exogenous NO role in regulating myoblast differentiation through the oxidative stress signaling pathway. Through this work, we can suggest that exogenous NO can help in cell differentiation and tissue regeneration, which provides new possibilities for plasma medicine.
format	article
author	Naresh Kumar Priyanka Shaw Han Sup Uhm Eun Ha Choi Pankaj Attri
author_facet	Naresh Kumar Priyanka Shaw Han Sup Uhm Eun Ha Choi Pankaj Attri
author_sort	Naresh Kumar
title	Influence of Nitric Oxide generated through microwave plasma on L6 skeletal muscle cell myogenesis via oxidative signaling pathways
title_short	Influence of Nitric Oxide generated through microwave plasma on L6 skeletal muscle cell myogenesis via oxidative signaling pathways
title_full	Influence of Nitric Oxide generated through microwave plasma on L6 skeletal muscle cell myogenesis via oxidative signaling pathways
title_fullStr	Influence of Nitric Oxide generated through microwave plasma on L6 skeletal muscle cell myogenesis via oxidative signaling pathways
title_full_unstemmed	Influence of Nitric Oxide generated through microwave plasma on L6 skeletal muscle cell myogenesis via oxidative signaling pathways
title_sort	influence of nitric oxide generated through microwave plasma on l6 skeletal muscle cell myogenesis via oxidative signaling pathways
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/357173c200b54f4e881ca044fb7d299c
work_keys_str_mv	AT nareshkumar influenceofnitricoxidegeneratedthroughmicrowaveplasmaonl6skeletalmusclecellmyogenesisviaoxidativesignalingpathways AT priyankashaw influenceofnitricoxidegeneratedthroughmicrowaveplasmaonl6skeletalmusclecellmyogenesisviaoxidativesignalingpathways AT hansupuhm influenceofnitricoxidegeneratedthroughmicrowaveplasmaonl6skeletalmusclecellmyogenesisviaoxidativesignalingpathways AT eunhachoi influenceofnitricoxidegeneratedthroughmicrowaveplasmaonl6skeletalmusclecellmyogenesisviaoxidativesignalingpathways AT pankajattri influenceofnitricoxidegeneratedthroughmicrowaveplasmaonl6skeletalmusclecellmyogenesisviaoxidativesignalingpathways
_version_	1718394326439952384

Influence of Nitric Oxide generated through microwave plasma on L6 skeletal muscle cell myogenesis via oxidative signaling pathways

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