Short ROSE-like RNA thermometers control IbpA synthesis in Pseudomonas species.

The bacterial small heat shock protein IbpA protects client proteins from aggregation. Due to redundancy in the cellular chaperone network, deletion of the ibpA gene often leads to only a mild or no phenotypic defect. In this study, we show that a Pseudomonas putida ibpA deletion mutant has a severe...

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Autores principales:	Stefanie S Krajewski, Miriam Nagel, Franz Narberhaus
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3578585ed6184531968e4dd0a11f577f
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spelling	oai:doaj.org-article:3578585ed6184531968e4dd0a11f577f2021-11-18T07:43:25ZShort ROSE-like RNA thermometers control IbpA synthesis in Pseudomonas species.1932-620310.1371/journal.pone.0065168https://doaj.org/article/3578585ed6184531968e4dd0a11f577f2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23741480/?tool=EBIhttps://doaj.org/toc/1932-6203The bacterial small heat shock protein IbpA protects client proteins from aggregation. Due to redundancy in the cellular chaperone network, deletion of the ibpA gene often leads to only a mild or no phenotypic defect. In this study, we show that a Pseudomonas putida ibpA deletion mutant has a severe growth defect under heat stress conditions and reduced survival during recovery revealing a critical role of IbpA in heat tolerance. Transcription of the ibpA gene depends on the alternative heat shock sigma factor σ(32). Production of IbpA protein only at heat shock temperatures suggested additional translational control. We conducted a comprehensive structural and functional analysis of the 5' untranslated regions of the ibpA genes from P. putida and Pseudomonas aeruginosa. Both contain a ROSE-type RNA thermometer that is substantially shorter and simpler than previously reported ROSE elements. Comprised of two hairpin structures only, they inhibit translation at low temperature and permit translation initiation after a temperature upshift. Both elements regulate reporter gene expression in Escherichia coli and ribosome binding in vitro in a temperature-dependent manner. Structure probing revealed local melting of the second hairpin whereas the first hairpin remained unaffected. High sequence and structure conservation of pseudomonal ibpA untranslated regions and their ability to confer thermoregulation in vivo suggest that short ROSE-like thermometers are commonly used to control IbpA synthesis in Pseudomonas species.Stefanie S KrajewskiMiriam NagelFranz NarberhausPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e65168 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stefanie S Krajewski Miriam Nagel Franz Narberhaus Short ROSE-like RNA thermometers control IbpA synthesis in Pseudomonas species.
description	The bacterial small heat shock protein IbpA protects client proteins from aggregation. Due to redundancy in the cellular chaperone network, deletion of the ibpA gene often leads to only a mild or no phenotypic defect. In this study, we show that a Pseudomonas putida ibpA deletion mutant has a severe growth defect under heat stress conditions and reduced survival during recovery revealing a critical role of IbpA in heat tolerance. Transcription of the ibpA gene depends on the alternative heat shock sigma factor σ(32). Production of IbpA protein only at heat shock temperatures suggested additional translational control. We conducted a comprehensive structural and functional analysis of the 5' untranslated regions of the ibpA genes from P. putida and Pseudomonas aeruginosa. Both contain a ROSE-type RNA thermometer that is substantially shorter and simpler than previously reported ROSE elements. Comprised of two hairpin structures only, they inhibit translation at low temperature and permit translation initiation after a temperature upshift. Both elements regulate reporter gene expression in Escherichia coli and ribosome binding in vitro in a temperature-dependent manner. Structure probing revealed local melting of the second hairpin whereas the first hairpin remained unaffected. High sequence and structure conservation of pseudomonal ibpA untranslated regions and their ability to confer thermoregulation in vivo suggest that short ROSE-like thermometers are commonly used to control IbpA synthesis in Pseudomonas species.
format	article
author	Stefanie S Krajewski Miriam Nagel Franz Narberhaus
author_facet	Stefanie S Krajewski Miriam Nagel Franz Narberhaus
author_sort	Stefanie S Krajewski
title	Short ROSE-like RNA thermometers control IbpA synthesis in Pseudomonas species.
title_short	Short ROSE-like RNA thermometers control IbpA synthesis in Pseudomonas species.
title_full	Short ROSE-like RNA thermometers control IbpA synthesis in Pseudomonas species.
title_fullStr	Short ROSE-like RNA thermometers control IbpA synthesis in Pseudomonas species.
title_full_unstemmed	Short ROSE-like RNA thermometers control IbpA synthesis in Pseudomonas species.
title_sort	short rose-like rna thermometers control ibpa synthesis in pseudomonas species.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/3578585ed6184531968e4dd0a11f577f
work_keys_str_mv	AT stefanieskrajewski shortroselikernathermometerscontrolibpasynthesisinpseudomonasspecies AT miriamnagel shortroselikernathermometerscontrolibpasynthesisinpseudomonasspecies AT franznarberhaus shortroselikernathermometerscontrolibpasynthesisinpseudomonasspecies
_version_	1718423027397427200

Short ROSE-like RNA thermometers control IbpA synthesis in Pseudomonas species.

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