Mechanical compliance and immunological compatibility of fixative-free decellularized/cryopreserved human pericardium.
<h4>Background</h4>The pericardial tissue is commonly used to produce bio-prosthetic cardiac valves and patches in cardiac surgery. The procedures adopted to prepare this tissue consist in treatment with aldehydes, which do not prevent post-graft tissue calcification due to incomplete xe...
Guardado en:
| Autores principales: | , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2013
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/357eabc4e45a449eb5f8b1a7116e660f |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:357eabc4e45a449eb5f8b1a7116e660f |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:357eabc4e45a449eb5f8b1a7116e660f2021-11-18T07:45:02ZMechanical compliance and immunological compatibility of fixative-free decellularized/cryopreserved human pericardium.1932-620310.1371/journal.pone.0064769https://doaj.org/article/357eabc4e45a449eb5f8b1a7116e660f2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23705010/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The pericardial tissue is commonly used to produce bio-prosthetic cardiac valves and patches in cardiac surgery. The procedures adopted to prepare this tissue consist in treatment with aldehydes, which do not prevent post-graft tissue calcification due to incomplete xeno-antigens removal. The adoption of fixative-free decellularization protocols has been therefore suggested to overcome this limitation. Although promising, the decellularized pericardium has not yet used in clinics, due to the absence of proofs indicating that the decellularization and cryopreservation procedures can effectively preserve the mechanical properties and the immunologic compatibility of the tissue.<h4>Principal findings</h4>The aim of the present work was to validate a procedure to prepare decellularized/cryopreserved human pericardium which may be implemented into cardiovascular homograft tissue Banks. The method employed to decellularize the tissue completely removed the cells without affecting ECM structure; furthermore, uniaxial tensile loading tests revealed an equivalent resistance of the decellularized tissue to strain, before and after the cryopreservation, in comparison with the fresh tissue. Finally, immunological compatibility, showed a minimized host immune cells invasion and low levels of systemic inflammation, as assessed by tissue transplantation into immune-competent mice.<h4>Conclusions</h4>Our results indicate, for the first time, that fixative-free decellularized pericardium from cadaveric tissue donors can be banked according to Tissue Repository-approved procedures without compromising its mechanical properties and immunological tolerance. This tissue can be therefore treated as a safe homograft for cardiac surgery.Maria Cristina VinciGiulio TessitoreLaura CastiglioniFrancesca PrandiMonica SonciniRosaria SantoroFilippo ConsoloFrancesca ColazzoBarbara MicheliLuigi SironiGianluca PolvaniMaurizio PescePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e64769 (2013) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Maria Cristina Vinci Giulio Tessitore Laura Castiglioni Francesca Prandi Monica Soncini Rosaria Santoro Filippo Consolo Francesca Colazzo Barbara Micheli Luigi Sironi Gianluca Polvani Maurizio Pesce Mechanical compliance and immunological compatibility of fixative-free decellularized/cryopreserved human pericardium. |
| description |
<h4>Background</h4>The pericardial tissue is commonly used to produce bio-prosthetic cardiac valves and patches in cardiac surgery. The procedures adopted to prepare this tissue consist in treatment with aldehydes, which do not prevent post-graft tissue calcification due to incomplete xeno-antigens removal. The adoption of fixative-free decellularization protocols has been therefore suggested to overcome this limitation. Although promising, the decellularized pericardium has not yet used in clinics, due to the absence of proofs indicating that the decellularization and cryopreservation procedures can effectively preserve the mechanical properties and the immunologic compatibility of the tissue.<h4>Principal findings</h4>The aim of the present work was to validate a procedure to prepare decellularized/cryopreserved human pericardium which may be implemented into cardiovascular homograft tissue Banks. The method employed to decellularize the tissue completely removed the cells without affecting ECM structure; furthermore, uniaxial tensile loading tests revealed an equivalent resistance of the decellularized tissue to strain, before and after the cryopreservation, in comparison with the fresh tissue. Finally, immunological compatibility, showed a minimized host immune cells invasion and low levels of systemic inflammation, as assessed by tissue transplantation into immune-competent mice.<h4>Conclusions</h4>Our results indicate, for the first time, that fixative-free decellularized pericardium from cadaveric tissue donors can be banked according to Tissue Repository-approved procedures without compromising its mechanical properties and immunological tolerance. This tissue can be therefore treated as a safe homograft for cardiac surgery. |
| format |
article |
| author |
Maria Cristina Vinci Giulio Tessitore Laura Castiglioni Francesca Prandi Monica Soncini Rosaria Santoro Filippo Consolo Francesca Colazzo Barbara Micheli Luigi Sironi Gianluca Polvani Maurizio Pesce |
| author_facet |
Maria Cristina Vinci Giulio Tessitore Laura Castiglioni Francesca Prandi Monica Soncini Rosaria Santoro Filippo Consolo Francesca Colazzo Barbara Micheli Luigi Sironi Gianluca Polvani Maurizio Pesce |
| author_sort |
Maria Cristina Vinci |
| title |
Mechanical compliance and immunological compatibility of fixative-free decellularized/cryopreserved human pericardium. |
| title_short |
Mechanical compliance and immunological compatibility of fixative-free decellularized/cryopreserved human pericardium. |
| title_full |
Mechanical compliance and immunological compatibility of fixative-free decellularized/cryopreserved human pericardium. |
| title_fullStr |
Mechanical compliance and immunological compatibility of fixative-free decellularized/cryopreserved human pericardium. |
| title_full_unstemmed |
Mechanical compliance and immunological compatibility of fixative-free decellularized/cryopreserved human pericardium. |
| title_sort |
mechanical compliance and immunological compatibility of fixative-free decellularized/cryopreserved human pericardium. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/357eabc4e45a449eb5f8b1a7116e660f |
| work_keys_str_mv |
AT mariacristinavinci mechanicalcomplianceandimmunologicalcompatibilityoffixativefreedecellularizedcryopreservedhumanpericardium AT giuliotessitore mechanicalcomplianceandimmunologicalcompatibilityoffixativefreedecellularizedcryopreservedhumanpericardium AT lauracastiglioni mechanicalcomplianceandimmunologicalcompatibilityoffixativefreedecellularizedcryopreservedhumanpericardium AT francescaprandi mechanicalcomplianceandimmunologicalcompatibilityoffixativefreedecellularizedcryopreservedhumanpericardium AT monicasoncini mechanicalcomplianceandimmunologicalcompatibilityoffixativefreedecellularizedcryopreservedhumanpericardium AT rosariasantoro mechanicalcomplianceandimmunologicalcompatibilityoffixativefreedecellularizedcryopreservedhumanpericardium AT filippoconsolo mechanicalcomplianceandimmunologicalcompatibilityoffixativefreedecellularizedcryopreservedhumanpericardium AT francescacolazzo mechanicalcomplianceandimmunologicalcompatibilityoffixativefreedecellularizedcryopreservedhumanpericardium AT barbaramicheli mechanicalcomplianceandimmunologicalcompatibilityoffixativefreedecellularizedcryopreservedhumanpericardium AT luigisironi mechanicalcomplianceandimmunologicalcompatibilityoffixativefreedecellularizedcryopreservedhumanpericardium AT gianlucapolvani mechanicalcomplianceandimmunologicalcompatibilityoffixativefreedecellularizedcryopreservedhumanpericardium AT mauriziopesce mechanicalcomplianceandimmunologicalcompatibilityoffixativefreedecellularizedcryopreservedhumanpericardium |
| _version_ |
1718423027627065344 |