CD5 Surface Expression Marks Intravascular Human Innate Lymphoid Cells That Have a Distinct Ontogeny and Migrate to the Lung

Innate lymphoid cells (ILCs) contribute to immune defense, yet it is poorly understood how ILCs develop and are strategically positioned in the lung. This applies especially to human ILCs due to the difficulty of studying them in vivo. Here we investigated the ontogeny and migration of human ILCs in...

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Autores principales:	Arlisa Alisjahbana, Yu Gao, Natalie Sleiers, Elza Evren, Demi Brownlie, Andreas von Kries, Carl Jorns, Nicole Marquardt, Jakob Michaëlsson, Tim Willinger
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	innate lymphoid cells (ILC) lung migration ontogeny humanized mice Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/3581d61e11c14a16a813b6307072b0c7
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spelling	oai:doaj.org-article:3581d61e11c14a16a813b6307072b0c72021-11-19T10:52:26ZCD5 Surface Expression Marks Intravascular Human Innate Lymphoid Cells That Have a Distinct Ontogeny and Migrate to the Lung1664-322410.3389/fimmu.2021.752104https://doaj.org/article/3581d61e11c14a16a813b6307072b0c72021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.752104/fullhttps://doaj.org/toc/1664-3224Innate lymphoid cells (ILCs) contribute to immune defense, yet it is poorly understood how ILCs develop and are strategically positioned in the lung. This applies especially to human ILCs due to the difficulty of studying them in vivo. Here we investigated the ontogeny and migration of human ILCs in vivo with a humanized mouse model (“MISTRG”) expressing human cytokines. In addition to known tissue-resident ILC subsets, we discovered CD5-expressing ILCs that predominantly resided within the lung vasculature and in the circulation. CD5+ ILCs contained IFNγ-producing mature ILC1s as well as immature ILCs that produced ILC effector cytokines under polarizing conditions in vitro. CD5+ ILCs had a distinct ontogeny compared to conventional CD5- ILCs because they first appeared in the thymus, spleen and liver rather than in the bone marrow after transplantation of MISTRG mice with human CD34+ hematopoietic stem and progenitor cells. Due to their strategic location, human CD5+ ILCs could serve as blood-borne sentinels, ready to be recruited into the lung to respond to environmental challenges. This work emphasizes the uniqueness of human CD5+ ILCs in terms of their anatomical localization and developmental origin compared to well-studied CD5- ILCs.Arlisa AlisjahbanaYu GaoNatalie SleiersElza EvrenDemi BrownlieAndreas von KriesCarl JornsCarl JornsNicole MarquardtJakob MichaëlssonTim WillingerFrontiers Media S.A.articleinnate lymphoid cells (ILC)lungmigrationontogenyhumanized miceImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	innate lymphoid cells (ILC) lung migration ontogeny humanized mice Immunologic diseases. Allergy RC581-607
spellingShingle	innate lymphoid cells (ILC) lung migration ontogeny humanized mice Immunologic diseases. Allergy RC581-607 Arlisa Alisjahbana Yu Gao Natalie Sleiers Elza Evren Demi Brownlie Andreas von Kries Carl Jorns Carl Jorns Nicole Marquardt Jakob Michaëlsson Tim Willinger CD5 Surface Expression Marks Intravascular Human Innate Lymphoid Cells That Have a Distinct Ontogeny and Migrate to the Lung
description	Innate lymphoid cells (ILCs) contribute to immune defense, yet it is poorly understood how ILCs develop and are strategically positioned in the lung. This applies especially to human ILCs due to the difficulty of studying them in vivo. Here we investigated the ontogeny and migration of human ILCs in vivo with a humanized mouse model (“MISTRG”) expressing human cytokines. In addition to known tissue-resident ILC subsets, we discovered CD5-expressing ILCs that predominantly resided within the lung vasculature and in the circulation. CD5+ ILCs contained IFNγ-producing mature ILC1s as well as immature ILCs that produced ILC effector cytokines under polarizing conditions in vitro. CD5+ ILCs had a distinct ontogeny compared to conventional CD5- ILCs because they first appeared in the thymus, spleen and liver rather than in the bone marrow after transplantation of MISTRG mice with human CD34+ hematopoietic stem and progenitor cells. Due to their strategic location, human CD5+ ILCs could serve as blood-borne sentinels, ready to be recruited into the lung to respond to environmental challenges. This work emphasizes the uniqueness of human CD5+ ILCs in terms of their anatomical localization and developmental origin compared to well-studied CD5- ILCs.
format	article
author	Arlisa Alisjahbana Yu Gao Natalie Sleiers Elza Evren Demi Brownlie Andreas von Kries Carl Jorns Carl Jorns Nicole Marquardt Jakob Michaëlsson Tim Willinger
author_facet	Arlisa Alisjahbana Yu Gao Natalie Sleiers Elza Evren Demi Brownlie Andreas von Kries Carl Jorns Carl Jorns Nicole Marquardt Jakob Michaëlsson Tim Willinger
author_sort	Arlisa Alisjahbana
title	CD5 Surface Expression Marks Intravascular Human Innate Lymphoid Cells That Have a Distinct Ontogeny and Migrate to the Lung
title_short	CD5 Surface Expression Marks Intravascular Human Innate Lymphoid Cells That Have a Distinct Ontogeny and Migrate to the Lung
title_full	CD5 Surface Expression Marks Intravascular Human Innate Lymphoid Cells That Have a Distinct Ontogeny and Migrate to the Lung
title_fullStr	CD5 Surface Expression Marks Intravascular Human Innate Lymphoid Cells That Have a Distinct Ontogeny and Migrate to the Lung
title_full_unstemmed	CD5 Surface Expression Marks Intravascular Human Innate Lymphoid Cells That Have a Distinct Ontogeny and Migrate to the Lung
title_sort	cd5 surface expression marks intravascular human innate lymphoid cells that have a distinct ontogeny and migrate to the lung
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/3581d61e11c14a16a813b6307072b0c7
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CD5 Surface Expression Marks Intravascular Human Innate Lymphoid Cells That Have a Distinct Ontogeny and Migrate to the Lung

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