Seven day pre-analytical stability of serum and plasma neurofilament light chain

Abstract Neurofilament light chain (NfL) has emerged as a biomarker of neuroaxonal damage in several neurologic conditions. With increasing availability of fourth-generation immunoassays detecting NfL in blood, aspects of pre-analytical stability of this biomarker remain unanswered. This study inves...

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Autores principales:	Patrick Altmann, Markus Ponleitner, Paulus Stefan Rommer, Helmuth Haslacher, Patrick Mucher, Fritz Leutmezer, Axel Petzold, Christoph Wotawa, Rupert Lanzenberger, Thomas Berger, Henrik Zetterberg, Gabriel Bsteh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/358a90b1ab554193b676f82369723acf
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spelling	oai:doaj.org-article:358a90b1ab554193b676f82369723acf2021-12-02T16:53:20ZSeven day pre-analytical stability of serum and plasma neurofilament light chain10.1038/s41598-021-90639-z2045-2322https://doaj.org/article/358a90b1ab554193b676f82369723acf2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90639-zhttps://doaj.org/toc/2045-2322Abstract Neurofilament light chain (NfL) has emerged as a biomarker of neuroaxonal damage in several neurologic conditions. With increasing availability of fourth-generation immunoassays detecting NfL in blood, aspects of pre-analytical stability of this biomarker remain unanswered. This study investigated NfL concentrations in serum and plasma samples of 32 patients with neurological diagnoses using state of the art Simoa technology. We tested the effect of delayed freezing of up to 7 days and statistically determined stability and validity of measured concentrations. We found concentrations of NfL in serum and plasma to remain stable at room temperature when processing of samples is delayed up to 7 days (serum: mean absolute difference 0.9 pg/mL, intraindividual variation 1.2%; plasma: mean absolute difference 0.5 pg/mL, intraindividual variation 1.3%). Consistency of these results was nearly perfect for serum and excellent for plasma (intraclass correlation coefficients 0.99 and 0.94, respectively). In conclusion, the soluble serum and plasma NfL concentration remains stable when unprocessed blood samples are stored up to 7 days at room temperature. This information is essential for ensuring reliable study protocols, for example, when shipment of fresh samples is needed.Patrick AltmannMarkus PonleitnerPaulus Stefan RommerHelmuth HaslacherPatrick MucherFritz LeutmezerAxel PetzoldChristoph WotawaRupert LanzenbergerThomas BergerHenrik ZetterbergGabriel BstehNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Patrick Altmann Markus Ponleitner Paulus Stefan Rommer Helmuth Haslacher Patrick Mucher Fritz Leutmezer Axel Petzold Christoph Wotawa Rupert Lanzenberger Thomas Berger Henrik Zetterberg Gabriel Bsteh Seven day pre-analytical stability of serum and plasma neurofilament light chain
description	Abstract Neurofilament light chain (NfL) has emerged as a biomarker of neuroaxonal damage in several neurologic conditions. With increasing availability of fourth-generation immunoassays detecting NfL in blood, aspects of pre-analytical stability of this biomarker remain unanswered. This study investigated NfL concentrations in serum and plasma samples of 32 patients with neurological diagnoses using state of the art Simoa technology. We tested the effect of delayed freezing of up to 7 days and statistically determined stability and validity of measured concentrations. We found concentrations of NfL in serum and plasma to remain stable at room temperature when processing of samples is delayed up to 7 days (serum: mean absolute difference 0.9 pg/mL, intraindividual variation 1.2%; plasma: mean absolute difference 0.5 pg/mL, intraindividual variation 1.3%). Consistency of these results was nearly perfect for serum and excellent for plasma (intraclass correlation coefficients 0.99 and 0.94, respectively). In conclusion, the soluble serum and plasma NfL concentration remains stable when unprocessed blood samples are stored up to 7 days at room temperature. This information is essential for ensuring reliable study protocols, for example, when shipment of fresh samples is needed.
format	article
author	Patrick Altmann Markus Ponleitner Paulus Stefan Rommer Helmuth Haslacher Patrick Mucher Fritz Leutmezer Axel Petzold Christoph Wotawa Rupert Lanzenberger Thomas Berger Henrik Zetterberg Gabriel Bsteh
author_facet	Patrick Altmann Markus Ponleitner Paulus Stefan Rommer Helmuth Haslacher Patrick Mucher Fritz Leutmezer Axel Petzold Christoph Wotawa Rupert Lanzenberger Thomas Berger Henrik Zetterberg Gabriel Bsteh
author_sort	Patrick Altmann
title	Seven day pre-analytical stability of serum and plasma neurofilament light chain
title_short	Seven day pre-analytical stability of serum and plasma neurofilament light chain
title_full	Seven day pre-analytical stability of serum and plasma neurofilament light chain
title_fullStr	Seven day pre-analytical stability of serum and plasma neurofilament light chain
title_full_unstemmed	Seven day pre-analytical stability of serum and plasma neurofilament light chain
title_sort	seven day pre-analytical stability of serum and plasma neurofilament light chain
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/358a90b1ab554193b676f82369723acf
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Seven day pre-analytical stability of serum and plasma neurofilament light chain

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