TNF-related apoptosis-inducing ligand deficiency enhances survival in murine colon ascendens stent peritonitis

Katharina Beyer,1 Laura Stollhof,1 Christian Poetschke,2 Wolfram von Bernstorff,1 Lars Ivo Partecke,1 Stephan Diedrich,1 Stefan Maier,1 Barbara M Bröker,2 Claus-Dieter Heidecke1 1Department of General, Visceral, Thoracic, and Vascular Surgery, 2Institute of Immunology, University of Greifsw...

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Autores principales:	Beyer K, Stollhof L, Poetschke C, von Bernstorff W, Partecke LI, Diedrich S, Maier S, Bröker BM, Heidecke CD
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Colon Ascendens Stent Peritonitis mice peritonitis sepsis TRAIL Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/3591f27aba174abd8b1228697de54700
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spelling	oai:doaj.org-article:3591f27aba174abd8b1228697de547002021-12-02T04:07:43ZTNF-related apoptosis-inducing ligand deficiency enhances survival in murine colon ascendens stent peritonitis1178-7031https://doaj.org/article/3591f27aba174abd8b1228697de547002016-06-01T00:00:00Zhttps://www.dovepress.com/tnf-related-apoptosis-inducing-ligand-deficiency-enhances-survival-in--peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Katharina Beyer,1 Laura Stollhof,1 Christian Poetschke,2 Wolfram von Bernstorff,1 Lars Ivo Partecke,1 Stephan Diedrich,1 Stefan Maier,1 Barbara M Bröker,2 Claus-Dieter Heidecke1 1Department of General, Visceral, Thoracic, and Vascular Surgery, 2Institute of Immunology, University of Greifswald, Greifswald, GermanyBackground: Apart from inducing apoptosis in tumor cells, tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) influences inflammatory reactions. Murine colon ascendens stent peritonitis (CASP) represents a model of diffuse peritonitis. Recently, it has been demonstrated that administration of exogenous TRAIL not only induces apoptosis in neutrophils but also enhances survival in this model. The aim of this study was to examine the impact of genetic TRAIL deficiency on the course of CASP.Methods: Peritonitis was induced in 6- to 8-week-old female TRAIL−/− mice as well as in wild-type mice. The sepsis severity score and survival of mice were monitored. Bacterial loads in blood as well as in the lymphoid organs were examined. Additionally, the number of apoptotic cells within the lymphoid organs was determined.Results: As early as 8 hours postinduction of CASP, TRAIL−/− mice were significantly more affected by sepsis than wild-type mice, as measured by the sepsis severity score. However, during the further course of sepsis, TRAIL deficiency led to significantly decreased sepsis severity scores, resulting in an enhanced overall survival in TRAIL−/− mice. The better survival of TRAIL−/− mice was accompanied by a decreased bacterial load within the blood. In marked contrast, the number of apoptotic cells within the lymphoid organs was highly increased in TRAIL−/− mice 20 hours after induction of CASP.Conclusion: Hence, exogenous and endogenous TRAIL is protective during the early phase of sepsis, while endogenous TRAIL appears to be detrimental in the later course of this disease.Keywords: CASP, mice, sepsis, TRAIL, inflammationBeyer KStollhof LPoetschke Cvon Bernstorff WPartecke LIDiedrich SMaier SBröker BMHeidecke CDDove Medical PressarticleColon Ascendens Stent PeritonitismiceperitonitissepsisTRAILPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol 2016, Iss Issue 1, Pp 103-113 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Colon Ascendens Stent Peritonitis mice peritonitis sepsis TRAIL Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	Colon Ascendens Stent Peritonitis mice peritonitis sepsis TRAIL Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Beyer K Stollhof L Poetschke C von Bernstorff W Partecke LI Diedrich S Maier S Bröker BM Heidecke CD TNF-related apoptosis-inducing ligand deficiency enhances survival in murine colon ascendens stent peritonitis
description	Katharina Beyer,1 Laura Stollhof,1 Christian Poetschke,2 Wolfram von Bernstorff,1 Lars Ivo Partecke,1 Stephan Diedrich,1 Stefan Maier,1 Barbara M Bröker,2 Claus-Dieter Heidecke1 1Department of General, Visceral, Thoracic, and Vascular Surgery, 2Institute of Immunology, University of Greifswald, Greifswald, GermanyBackground: Apart from inducing apoptosis in tumor cells, tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) influences inflammatory reactions. Murine colon ascendens stent peritonitis (CASP) represents a model of diffuse peritonitis. Recently, it has been demonstrated that administration of exogenous TRAIL not only induces apoptosis in neutrophils but also enhances survival in this model. The aim of this study was to examine the impact of genetic TRAIL deficiency on the course of CASP.Methods: Peritonitis was induced in 6- to 8-week-old female TRAIL−/− mice as well as in wild-type mice. The sepsis severity score and survival of mice were monitored. Bacterial loads in blood as well as in the lymphoid organs were examined. Additionally, the number of apoptotic cells within the lymphoid organs was determined.Results: As early as 8 hours postinduction of CASP, TRAIL−/− mice were significantly more affected by sepsis than wild-type mice, as measured by the sepsis severity score. However, during the further course of sepsis, TRAIL deficiency led to significantly decreased sepsis severity scores, resulting in an enhanced overall survival in TRAIL−/− mice. The better survival of TRAIL−/− mice was accompanied by a decreased bacterial load within the blood. In marked contrast, the number of apoptotic cells within the lymphoid organs was highly increased in TRAIL−/− mice 20 hours after induction of CASP.Conclusion: Hence, exogenous and endogenous TRAIL is protective during the early phase of sepsis, while endogenous TRAIL appears to be detrimental in the later course of this disease.Keywords: CASP, mice, sepsis, TRAIL, inflammation
format	article
author	Beyer K Stollhof L Poetschke C von Bernstorff W Partecke LI Diedrich S Maier S Bröker BM Heidecke CD
author_facet	Beyer K Stollhof L Poetschke C von Bernstorff W Partecke LI Diedrich S Maier S Bröker BM Heidecke CD
author_sort	Beyer K
title	TNF-related apoptosis-inducing ligand deficiency enhances survival in murine colon ascendens stent peritonitis
title_short	TNF-related apoptosis-inducing ligand deficiency enhances survival in murine colon ascendens stent peritonitis
title_full	TNF-related apoptosis-inducing ligand deficiency enhances survival in murine colon ascendens stent peritonitis
title_fullStr	TNF-related apoptosis-inducing ligand deficiency enhances survival in murine colon ascendens stent peritonitis
title_full_unstemmed	TNF-related apoptosis-inducing ligand deficiency enhances survival in murine colon ascendens stent peritonitis
title_sort	tnf-related apoptosis-inducing ligand deficiency enhances survival in murine colon ascendens stent peritonitis
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/3591f27aba174abd8b1228697de54700
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TNF-related apoptosis-inducing ligand deficiency enhances survival in murine colon ascendens stent peritonitis

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