Multiple candidate effectors from the oomycete pathogen Hyaloperonospora arabidopsidis suppress host plant immunity.
Oomycete pathogens cause diverse plant diseases. To successfully colonize their hosts, they deliver a suite of effector proteins that can attenuate plant defenses. In the oomycete downy mildews, effectors carry a signal peptide and an RxLR motif. Hyaloperonospora arabidopsidis (Hpa) causes downy mil...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2011
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/35933c994790406a8ab8418a11271733 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:35933c994790406a8ab8418a11271733 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:35933c994790406a8ab8418a112717332021-11-18T06:05:10ZMultiple candidate effectors from the oomycete pathogen Hyaloperonospora arabidopsidis suppress host plant immunity.1553-73661553-737410.1371/journal.ppat.1002348https://doaj.org/article/35933c994790406a8ab8418a112717332011-11-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22072967/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Oomycete pathogens cause diverse plant diseases. To successfully colonize their hosts, they deliver a suite of effector proteins that can attenuate plant defenses. In the oomycete downy mildews, effectors carry a signal peptide and an RxLR motif. Hyaloperonospora arabidopsidis (Hpa) causes downy mildew on the model plant Arabidopsis thaliana (Arabidopsis). We investigated if candidate effectors predicted in the genome sequence of Hpa isolate Emoy2 (HaRxLs) were able to manipulate host defenses in different Arabidopsis accessions. We developed a rapid and sensitive screening method to test HaRxLs by delivering them via the bacterial type-three secretion system (TTSS) of Pseudomonas syringae pv tomato DC3000-LUX (Pst-LUX) and assessing changes in Pst-LUX growth in planta on 12 Arabidopsis accessions. The majority (~70%) of the 64 candidates tested positively contributed to Pst-LUX growth on more than one accession indicating that Hpa virulence likely involves multiple effectors with weak accession-specific effects. Further screening with a Pst mutant (ΔCEL) showed that HaRxLs that allow enhanced Pst-LUX growth usually suppress callose deposition, a hallmark of pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI). We found that HaRxLs are rarely strong avirulence determinants. Although some decreased Pst-LUX growth in particular accessions, none activated macroscopic cell death. Fewer HaRxLs conferred enhanced Pst growth on turnip, a non-host for Hpa, while several reduced it, consistent with the idea that turnip's non-host resistance against Hpa could involve a combination of recognized HaRxLs and ineffective HaRxLs. We verified our results by constitutively expressing in Arabidopsis a sub-set of HaRxLs. Several transgenic lines showed increased susceptibility to Hpa and attenuation of Arabidopsis PTI responses, confirming the HaRxLs' role in Hpa virulence. This study shows TTSS screening system provides a useful tool to test whether candidate effectors from eukaryotic pathogens can suppress/trigger plant defense mechanisms and to rank their effectiveness prior to subsequent mechanistic investigation.Georgina FabroJens SteinbrennerMary CoatesNaveed IshaqueLaura BaxterDavid J StudholmeEvelyn KörnerRebecca L AllenSophie J M PiquerezAlejandra Rougon-CardosoDavid GreenshieldsRita LeiJorge L BadelMarie-Cecile CaillaudKee-Hoon SohnGuido Van den AckervekenJane E ParkerJim BeynonJonathan D G JonesPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 7, Iss 11, p e1002348 (2011) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 |
| spellingShingle |
Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Georgina Fabro Jens Steinbrenner Mary Coates Naveed Ishaque Laura Baxter David J Studholme Evelyn Körner Rebecca L Allen Sophie J M Piquerez Alejandra Rougon-Cardoso David Greenshields Rita Lei Jorge L Badel Marie-Cecile Caillaud Kee-Hoon Sohn Guido Van den Ackerveken Jane E Parker Jim Beynon Jonathan D G Jones Multiple candidate effectors from the oomycete pathogen Hyaloperonospora arabidopsidis suppress host plant immunity. |
| description |
Oomycete pathogens cause diverse plant diseases. To successfully colonize their hosts, they deliver a suite of effector proteins that can attenuate plant defenses. In the oomycete downy mildews, effectors carry a signal peptide and an RxLR motif. Hyaloperonospora arabidopsidis (Hpa) causes downy mildew on the model plant Arabidopsis thaliana (Arabidopsis). We investigated if candidate effectors predicted in the genome sequence of Hpa isolate Emoy2 (HaRxLs) were able to manipulate host defenses in different Arabidopsis accessions. We developed a rapid and sensitive screening method to test HaRxLs by delivering them via the bacterial type-three secretion system (TTSS) of Pseudomonas syringae pv tomato DC3000-LUX (Pst-LUX) and assessing changes in Pst-LUX growth in planta on 12 Arabidopsis accessions. The majority (~70%) of the 64 candidates tested positively contributed to Pst-LUX growth on more than one accession indicating that Hpa virulence likely involves multiple effectors with weak accession-specific effects. Further screening with a Pst mutant (ΔCEL) showed that HaRxLs that allow enhanced Pst-LUX growth usually suppress callose deposition, a hallmark of pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI). We found that HaRxLs are rarely strong avirulence determinants. Although some decreased Pst-LUX growth in particular accessions, none activated macroscopic cell death. Fewer HaRxLs conferred enhanced Pst growth on turnip, a non-host for Hpa, while several reduced it, consistent with the idea that turnip's non-host resistance against Hpa could involve a combination of recognized HaRxLs and ineffective HaRxLs. We verified our results by constitutively expressing in Arabidopsis a sub-set of HaRxLs. Several transgenic lines showed increased susceptibility to Hpa and attenuation of Arabidopsis PTI responses, confirming the HaRxLs' role in Hpa virulence. This study shows TTSS screening system provides a useful tool to test whether candidate effectors from eukaryotic pathogens can suppress/trigger plant defense mechanisms and to rank their effectiveness prior to subsequent mechanistic investigation. |
| format |
article |
| author |
Georgina Fabro Jens Steinbrenner Mary Coates Naveed Ishaque Laura Baxter David J Studholme Evelyn Körner Rebecca L Allen Sophie J M Piquerez Alejandra Rougon-Cardoso David Greenshields Rita Lei Jorge L Badel Marie-Cecile Caillaud Kee-Hoon Sohn Guido Van den Ackerveken Jane E Parker Jim Beynon Jonathan D G Jones |
| author_facet |
Georgina Fabro Jens Steinbrenner Mary Coates Naveed Ishaque Laura Baxter David J Studholme Evelyn Körner Rebecca L Allen Sophie J M Piquerez Alejandra Rougon-Cardoso David Greenshields Rita Lei Jorge L Badel Marie-Cecile Caillaud Kee-Hoon Sohn Guido Van den Ackerveken Jane E Parker Jim Beynon Jonathan D G Jones |
| author_sort |
Georgina Fabro |
| title |
Multiple candidate effectors from the oomycete pathogen Hyaloperonospora arabidopsidis suppress host plant immunity. |
| title_short |
Multiple candidate effectors from the oomycete pathogen Hyaloperonospora arabidopsidis suppress host plant immunity. |
| title_full |
Multiple candidate effectors from the oomycete pathogen Hyaloperonospora arabidopsidis suppress host plant immunity. |
| title_fullStr |
Multiple candidate effectors from the oomycete pathogen Hyaloperonospora arabidopsidis suppress host plant immunity. |
| title_full_unstemmed |
Multiple candidate effectors from the oomycete pathogen Hyaloperonospora arabidopsidis suppress host plant immunity. |
| title_sort |
multiple candidate effectors from the oomycete pathogen hyaloperonospora arabidopsidis suppress host plant immunity. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2011 |
| url |
https://doaj.org/article/35933c994790406a8ab8418a11271733 |
| work_keys_str_mv |
AT georginafabro multiplecandidateeffectorsfromtheoomycetepathogenhyaloperonosporaarabidopsidissuppresshostplantimmunity AT jenssteinbrenner multiplecandidateeffectorsfromtheoomycetepathogenhyaloperonosporaarabidopsidissuppresshostplantimmunity AT marycoates multiplecandidateeffectorsfromtheoomycetepathogenhyaloperonosporaarabidopsidissuppresshostplantimmunity AT naveedishaque multiplecandidateeffectorsfromtheoomycetepathogenhyaloperonosporaarabidopsidissuppresshostplantimmunity AT laurabaxter multiplecandidateeffectorsfromtheoomycetepathogenhyaloperonosporaarabidopsidissuppresshostplantimmunity AT davidjstudholme multiplecandidateeffectorsfromtheoomycetepathogenhyaloperonosporaarabidopsidissuppresshostplantimmunity AT evelynkorner multiplecandidateeffectorsfromtheoomycetepathogenhyaloperonosporaarabidopsidissuppresshostplantimmunity AT rebeccalallen multiplecandidateeffectorsfromtheoomycetepathogenhyaloperonosporaarabidopsidissuppresshostplantimmunity AT sophiejmpiquerez multiplecandidateeffectorsfromtheoomycetepathogenhyaloperonosporaarabidopsidissuppresshostplantimmunity AT alejandrarougoncardoso multiplecandidateeffectorsfromtheoomycetepathogenhyaloperonosporaarabidopsidissuppresshostplantimmunity AT davidgreenshields multiplecandidateeffectorsfromtheoomycetepathogenhyaloperonosporaarabidopsidissuppresshostplantimmunity AT ritalei multiplecandidateeffectorsfromtheoomycetepathogenhyaloperonosporaarabidopsidissuppresshostplantimmunity AT jorgelbadel multiplecandidateeffectorsfromtheoomycetepathogenhyaloperonosporaarabidopsidissuppresshostplantimmunity AT mariececilecaillaud multiplecandidateeffectorsfromtheoomycetepathogenhyaloperonosporaarabidopsidissuppresshostplantimmunity AT keehoonsohn multiplecandidateeffectorsfromtheoomycetepathogenhyaloperonosporaarabidopsidissuppresshostplantimmunity AT guidovandenackerveken multiplecandidateeffectorsfromtheoomycetepathogenhyaloperonosporaarabidopsidissuppresshostplantimmunity AT janeeparker multiplecandidateeffectorsfromtheoomycetepathogenhyaloperonosporaarabidopsidissuppresshostplantimmunity AT jimbeynon multiplecandidateeffectorsfromtheoomycetepathogenhyaloperonosporaarabidopsidissuppresshostplantimmunity AT jonathandgjones multiplecandidateeffectorsfromtheoomycetepathogenhyaloperonosporaarabidopsidissuppresshostplantimmunity |
| _version_ |
1718424595221970944 |