Susceptibility to Insecticides and Natural Infection in Aedes aegypti: An Initiative to Improve the Mosquito Control Actions in Boyacá, Colombia

Background: Integrated management strategies for dengue prevention and control have been the main way to decrease the transmission of arboviruses transmitted by 'A. aegypti' in Colombia. However, the increase of chikungunya (CHIKV), Zika, and dengue (DENV) fever cases suggests deficiencies...

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Autores principales:	Omar Cantillo-Barraza, Manuel Medina, Yurany Granada, Camilo Muñoz, Cesar Valverde, Fernando Cely, Paola González, Yovanny Mendoza, Sara Zuluaga, Omar Triana-Chavez
Formato:	article
Lenguaje:	EN
Publicado:	Ubiquity Press 2020
Materias:	Infectious and parasitic diseases RC109-216 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/3594c74f99ce4a45b3400585bca76421
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spelling	oai:doaj.org-article:3594c74f99ce4a45b3400585bca764212021-12-02T03:56:04ZSusceptibility to Insecticides and Natural Infection in Aedes aegypti: An Initiative to Improve the Mosquito Control Actions in Boyacá, Colombia2214-999610.5334/aogh.2805https://doaj.org/article/3594c74f99ce4a45b3400585bca764212020-08-01T00:00:00Zhttps://annalsofglobalhealth.org/articles/2805https://doaj.org/toc/2214-9996Background: Integrated management strategies for dengue prevention and control have been the main way to decrease the transmission of arboviruses transmitted by 'A. aegypti' in Colombia. However, the increase of chikungunya (CHIKV), Zika, and dengue (DENV) fever cases suggests deficiencies in vector control strategies in some regions from this country. Objective: This work aimed to establish a baseline susceptibility profile of 'A. aegypti' to insecticides, determine the presence of 'kdr' mutations associated with resistance to pyrethroids, and detect natural arbovirus infection in this vector from Moniquirá – Boyacá, one of the most endemic cities in Colombia. Methods: Mosquitos were collected in six neighborhoods, and colonies established in the laboratory. Susceptibility to malathion and lambda-cyhalothrin insecticides was evaluated, and we examined the point mutations present in portions of domains I, II, III, and IV of the sodium channel gene using a simple allele-specific PCR-based assay (AS-PCR). Findings: 'A. aegypti' from Moniquirá showed decreased susceptibility to pyrethroid insecticides, and kdr mutations 419L, 1016I, and 1558C with allelic frequencies of 0.39, 0.40 and 0.95, respectively, were observed. The minimal infection rate (MIR) to DENV-1 was 44.1, while to CHIKV was 14.7. Conclusions: We establish a baseline insecticide resistance, 'kdr' mutations, and arbovirus circulation, which contain the elements necessary for the consolidation of a local surveillance strategy with an early warning system and rational selection and rotation of insecticides.Omar Cantillo-BarrazaManuel MedinaYurany GranadaCamilo MuñozCesar ValverdeFernando CelyPaola GonzálezYovanny MendozaSara ZuluagaOmar Triana-ChavezUbiquity PressarticleInfectious and parasitic diseasesRC109-216Public aspects of medicineRA1-1270ENAnnals of Global Health, Vol 86, Iss 1 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Infectious and parasitic diseases RC109-216 Public aspects of medicine RA1-1270
spellingShingle	Infectious and parasitic diseases RC109-216 Public aspects of medicine RA1-1270 Omar Cantillo-Barraza Manuel Medina Yurany Granada Camilo Muñoz Cesar Valverde Fernando Cely Paola González Yovanny Mendoza Sara Zuluaga Omar Triana-Chavez Susceptibility to Insecticides and Natural Infection in Aedes aegypti: An Initiative to Improve the Mosquito Control Actions in Boyacá, Colombia
description	Background: Integrated management strategies for dengue prevention and control have been the main way to decrease the transmission of arboviruses transmitted by 'A. aegypti' in Colombia. However, the increase of chikungunya (CHIKV), Zika, and dengue (DENV) fever cases suggests deficiencies in vector control strategies in some regions from this country. Objective: This work aimed to establish a baseline susceptibility profile of 'A. aegypti' to insecticides, determine the presence of 'kdr' mutations associated with resistance to pyrethroids, and detect natural arbovirus infection in this vector from Moniquirá – Boyacá, one of the most endemic cities in Colombia. Methods: Mosquitos were collected in six neighborhoods, and colonies established in the laboratory. Susceptibility to malathion and lambda-cyhalothrin insecticides was evaluated, and we examined the point mutations present in portions of domains I, II, III, and IV of the sodium channel gene using a simple allele-specific PCR-based assay (AS-PCR). Findings: 'A. aegypti' from Moniquirá showed decreased susceptibility to pyrethroid insecticides, and kdr mutations 419L, 1016I, and 1558C with allelic frequencies of 0.39, 0.40 and 0.95, respectively, were observed. The minimal infection rate (MIR) to DENV-1 was 44.1, while to CHIKV was 14.7. Conclusions: We establish a baseline insecticide resistance, 'kdr' mutations, and arbovirus circulation, which contain the elements necessary for the consolidation of a local surveillance strategy with an early warning system and rational selection and rotation of insecticides.
format	article
author	Omar Cantillo-Barraza Manuel Medina Yurany Granada Camilo Muñoz Cesar Valverde Fernando Cely Paola González Yovanny Mendoza Sara Zuluaga Omar Triana-Chavez
author_facet	Omar Cantillo-Barraza Manuel Medina Yurany Granada Camilo Muñoz Cesar Valverde Fernando Cely Paola González Yovanny Mendoza Sara Zuluaga Omar Triana-Chavez
author_sort	Omar Cantillo-Barraza
title	Susceptibility to Insecticides and Natural Infection in Aedes aegypti: An Initiative to Improve the Mosquito Control Actions in Boyacá, Colombia
title_short	Susceptibility to Insecticides and Natural Infection in Aedes aegypti: An Initiative to Improve the Mosquito Control Actions in Boyacá, Colombia
title_full	Susceptibility to Insecticides and Natural Infection in Aedes aegypti: An Initiative to Improve the Mosquito Control Actions in Boyacá, Colombia
title_fullStr	Susceptibility to Insecticides and Natural Infection in Aedes aegypti: An Initiative to Improve the Mosquito Control Actions in Boyacá, Colombia
title_full_unstemmed	Susceptibility to Insecticides and Natural Infection in Aedes aegypti: An Initiative to Improve the Mosquito Control Actions in Boyacá, Colombia
title_sort	susceptibility to insecticides and natural infection in aedes aegypti: an initiative to improve the mosquito control actions in boyacá, colombia
publisher	Ubiquity Press
publishDate	2020
url	https://doaj.org/article/3594c74f99ce4a45b3400585bca76421
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Susceptibility to Insecticides and Natural Infection in Aedes aegypti: An Initiative to Improve the Mosquito Control Actions in Boyacá, Colombia

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