Selective laser sintering fabrication of nano-hydroxyapatite/poly-ε-caprolactone scaffolds for bone tissue engineering applications

Selective laser sintering fabrication of nano-hydroxyapatite/poly-ε-caprolactone scaffolds for bone tissue engineering applications

Yan Xia,1,* Panyu Zhou,1,* Xiaosong Cheng,1,* Yang Xie,1,* Chong Liang,2 Chao Li,1 Shuogui Xu1,2 1Department of Orthopedics, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China; 2Department of Neurosurgery, The 81 Hospital of People's Liberatio...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Xia Y, Zhou PY, Cheng XS, Xie Y, Liang C, Li C, Xu SG
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2013
Materias:
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/359c215f117c4c30bb8830032a4bb8ed
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:359c215f117c4c30bb8830032a4bb8ed
record_format dspace
spelling oai:doaj.org-article:359c215f117c4c30bb8830032a4bb8ed2021-12-02T06:46:36ZSelective laser sintering fabrication of nano-hydroxyapatite/poly-ε-caprolactone scaffolds for bone tissue engineering applications1176-91141178-2013https://doaj.org/article/359c215f117c4c30bb8830032a4bb8ed2013-11-01T00:00:00Zhttp://www.dovepress.com/selective-laser-sintering-fabrication-of-nano-hydroxyapatitepoly-epsil-a14873https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Yan Xia,1,* Panyu Zhou,1,* Xiaosong Cheng,1,* Yang Xie,1,* Chong Liang,2 Chao Li,1 Shuogui Xu1,2 1Department of Orthopedics, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China; 2Department of Neurosurgery, The 81 Hospital of People's Liberation Army of China, Nanjing, People's Republic of China *These authors contributed equally to this work Abstract: The regeneration of functional tissue in osseous defects is a formidable challenge in orthopedic surgery. In the present study, a novel biomimetic composite scaffold, here called nano-hydroxyapatite (HA)/poly-ε-caprolactone (PCL) was fabricated using a selective laser sintering technique. The macrostructure, morphology, and mechanical strength of the scaffolds were characterized. Scanning electronic microscopy (SEM) showed that the nano-HA/PCL scaffolds exhibited predesigned, well-ordered macropores and interconnected micropores. The scaffolds have a range of porosity from 78.54% to 70.31%, and a corresponding compressive strength of 1.38 MPa to 3.17 MPa. Human bone marrow stromal cells were seeded onto the nano-HA/PCL or PCL scaffolds and cultured for 28 days in vitro. As indicated by the level of cell attachment and proliferation, the nano-HA/PCL showed excellent biocompatibility, comparable to that of PCL scaffolds. The hydrophilicity, mineralization, alkaline phosphatase activity, and Alizarin Red S staining indicated that the nano-HA/PCL scaffolds are more bioactive than the PCL scaffolds in vitro. Measurements of recombinant human bone morphogenetic protein-2 (rhBMP-2) release kinetics showed that after nano-HA was added, the material increased the rate of rhBMP-2 release. To investigate the in vivo biocompatibility and osteogenesis of the composite scaffolds, both nano-HA/PCL scaffolds and PCL scaffolds were implanted in rabbit femur defects for 3, 6, and 9 weeks. The wounds were studied radiographically and histologically. The in vivo results showed that both nano-HA/PCL composite scaffolds and PCL scaffolds exhibited good biocompatibility. However, the nano-HA/PCL scaffolds enhanced the efficiency of new bone formation more than PCL scaffolds and fulfilled all the basic requirements of bone tissue engineering scaffolds. Thus, they show large potential for use in orthopedic and reconstructive surgery. Keywords: osseous defects, orthopedic surgery, biomimetic composite scaffold, reconstructive surgeryXia YZhou PYCheng XSXie YLiang CLi CXu SGDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss Issue 1, Pp 4197-4213 (2013)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Xia Y
Zhou PY
Cheng XS
Xie Y
Liang C
Li C
Xu SG
Selective laser sintering fabrication of nano-hydroxyapatite/poly-ε-caprolactone scaffolds for bone tissue engineering applications
description Yan Xia,1,* Panyu Zhou,1,* Xiaosong Cheng,1,* Yang Xie,1,* Chong Liang,2 Chao Li,1 Shuogui Xu1,2 1Department of Orthopedics, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China; 2Department of Neurosurgery, The 81 Hospital of People's Liberation Army of China, Nanjing, People's Republic of China *These authors contributed equally to this work Abstract: The regeneration of functional tissue in osseous defects is a formidable challenge in orthopedic surgery. In the present study, a novel biomimetic composite scaffold, here called nano-hydroxyapatite (HA)/poly-ε-caprolactone (PCL) was fabricated using a selective laser sintering technique. The macrostructure, morphology, and mechanical strength of the scaffolds were characterized. Scanning electronic microscopy (SEM) showed that the nano-HA/PCL scaffolds exhibited predesigned, well-ordered macropores and interconnected micropores. The scaffolds have a range of porosity from 78.54% to 70.31%, and a corresponding compressive strength of 1.38 MPa to 3.17 MPa. Human bone marrow stromal cells were seeded onto the nano-HA/PCL or PCL scaffolds and cultured for 28 days in vitro. As indicated by the level of cell attachment and proliferation, the nano-HA/PCL showed excellent biocompatibility, comparable to that of PCL scaffolds. The hydrophilicity, mineralization, alkaline phosphatase activity, and Alizarin Red S staining indicated that the nano-HA/PCL scaffolds are more bioactive than the PCL scaffolds in vitro. Measurements of recombinant human bone morphogenetic protein-2 (rhBMP-2) release kinetics showed that after nano-HA was added, the material increased the rate of rhBMP-2 release. To investigate the in vivo biocompatibility and osteogenesis of the composite scaffolds, both nano-HA/PCL scaffolds and PCL scaffolds were implanted in rabbit femur defects for 3, 6, and 9 weeks. The wounds were studied radiographically and histologically. The in vivo results showed that both nano-HA/PCL composite scaffolds and PCL scaffolds exhibited good biocompatibility. However, the nano-HA/PCL scaffolds enhanced the efficiency of new bone formation more than PCL scaffolds and fulfilled all the basic requirements of bone tissue engineering scaffolds. Thus, they show large potential for use in orthopedic and reconstructive surgery. Keywords: osseous defects, orthopedic surgery, biomimetic composite scaffold, reconstructive surgery
format article
author Xia Y
Zhou PY
Cheng XS
Xie Y
Liang C
Li C
Xu SG
author_facet Xia Y
Zhou PY
Cheng XS
Xie Y
Liang C
Li C
Xu SG
author_sort Xia Y
title Selective laser sintering fabrication of nano-hydroxyapatite/poly-ε-caprolactone scaffolds for bone tissue engineering applications
title_short Selective laser sintering fabrication of nano-hydroxyapatite/poly-ε-caprolactone scaffolds for bone tissue engineering applications
title_full Selective laser sintering fabrication of nano-hydroxyapatite/poly-ε-caprolactone scaffolds for bone tissue engineering applications
title_fullStr Selective laser sintering fabrication of nano-hydroxyapatite/poly-ε-caprolactone scaffolds for bone tissue engineering applications
title_full_unstemmed Selective laser sintering fabrication of nano-hydroxyapatite/poly-ε-caprolactone scaffolds for bone tissue engineering applications
title_sort selective laser sintering fabrication of nano-hydroxyapatite/poly-ε-caprolactone scaffolds for bone tissue engineering applications
publisher Dove Medical Press
publishDate 2013
url https://doaj.org/article/359c215f117c4c30bb8830032a4bb8ed
work_keys_str_mv AT xiay selectivelasersinteringfabricationofnanohydroxyapatitepolyepsiloncaprolactonescaffoldsforbonetissueengineeringapplications
AT zhoupy selectivelasersinteringfabricationofnanohydroxyapatitepolyepsiloncaprolactonescaffoldsforbonetissueengineeringapplications
AT chengxs selectivelasersinteringfabricationofnanohydroxyapatitepolyepsiloncaprolactonescaffoldsforbonetissueengineeringapplications
AT xiey selectivelasersinteringfabricationofnanohydroxyapatitepolyepsiloncaprolactonescaffoldsforbonetissueengineeringapplications
AT liangc selectivelasersinteringfabricationofnanohydroxyapatitepolyepsiloncaprolactonescaffoldsforbonetissueengineeringapplications
AT lic selectivelasersinteringfabricationofnanohydroxyapatitepolyepsiloncaprolactonescaffoldsforbonetissueengineeringapplications
AT xusg selectivelasersinteringfabricationofnanohydroxyapatitepolyepsiloncaprolactonescaffoldsforbonetissueengineeringapplications
_version_ 1718399746904686592

Ejemplares similares