Micropatterning of planar metal electrodes by vacuum filling microfluidic channel geometries

Abstract We present a simple, facile method to micropattern planar metal electrodes defined by the geometry of a microfluidic channel network template. By introducing aqueous solutions of metal into reversibly adhered PDMS devices by desiccation instead of flow, we are able to produce difficult to p...

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Autores principales:	Stelios Chatzimichail, Pashiini Supramaniam, Oscar Ces, Ali Salehi-Reyhani
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Microfluidic Channel Microwell Array Laser Micromachining Polydimethylsiloxane (PDMS) Electrical Lysis Medicine R Science Q
Acceso en línea:	https://doaj.org/article/359ed05fc23a4098b1752f2632814f45
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spelling	oai:doaj.org-article:359ed05fc23a4098b1752f2632814f452021-12-02T15:09:12ZMicropatterning of planar metal electrodes by vacuum filling microfluidic channel geometries10.1038/s41598-018-32706-62045-2322https://doaj.org/article/359ed05fc23a4098b1752f2632814f452018-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-32706-6https://doaj.org/toc/2045-2322Abstract We present a simple, facile method to micropattern planar metal electrodes defined by the geometry of a microfluidic channel network template. By introducing aqueous solutions of metal into reversibly adhered PDMS devices by desiccation instead of flow, we are able to produce difficult to pattern “dead end” or discontinuous features with ease. We characterize electrodes fabricated using this method and perform electrical lysis of mammalian cancer cells and demonstrate their use as part of an antibody capture assay for GFP. Cell lysis in microwell arrays is achieved using the electrodes and the protein released is detected using an antibody microarray. We show how the template channels used as part of the workflow for patterning the electrodes may be produced using photolithography-free methods, such as laser micromachining and PDMS master moulding, and demonstrate how the use of an immiscible phase may be employed to create electrode spacings on the order of 25–50 μm, that overcome the current resolution limits of such methods. This work demonstrates how the rapid prototyping of electrodes for use in total analysis systems can be achieved on the bench with little or no need for centralized facilities.Stelios ChatzimichailPashiini SupramaniamOscar CesAli Salehi-ReyhaniNature PortfolioarticleMicrofluidic ChannelMicrowell ArrayLaser MicromachiningPolydimethylsiloxane (PDMS)Electrical LysisMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microfluidic Channel Microwell Array Laser Micromachining Polydimethylsiloxane (PDMS) Electrical Lysis Medicine R Science Q
spellingShingle	Microfluidic Channel Microwell Array Laser Micromachining Polydimethylsiloxane (PDMS) Electrical Lysis Medicine R Science Q Stelios Chatzimichail Pashiini Supramaniam Oscar Ces Ali Salehi-Reyhani Micropatterning of planar metal electrodes by vacuum filling microfluidic channel geometries
description	Abstract We present a simple, facile method to micropattern planar metal electrodes defined by the geometry of a microfluidic channel network template. By introducing aqueous solutions of metal into reversibly adhered PDMS devices by desiccation instead of flow, we are able to produce difficult to pattern “dead end” or discontinuous features with ease. We characterize electrodes fabricated using this method and perform electrical lysis of mammalian cancer cells and demonstrate their use as part of an antibody capture assay for GFP. Cell lysis in microwell arrays is achieved using the electrodes and the protein released is detected using an antibody microarray. We show how the template channels used as part of the workflow for patterning the electrodes may be produced using photolithography-free methods, such as laser micromachining and PDMS master moulding, and demonstrate how the use of an immiscible phase may be employed to create electrode spacings on the order of 25–50 μm, that overcome the current resolution limits of such methods. This work demonstrates how the rapid prototyping of electrodes for use in total analysis systems can be achieved on the bench with little or no need for centralized facilities.
format	article
author	Stelios Chatzimichail Pashiini Supramaniam Oscar Ces Ali Salehi-Reyhani
author_facet	Stelios Chatzimichail Pashiini Supramaniam Oscar Ces Ali Salehi-Reyhani
author_sort	Stelios Chatzimichail
title	Micropatterning of planar metal electrodes by vacuum filling microfluidic channel geometries
title_short	Micropatterning of planar metal electrodes by vacuum filling microfluidic channel geometries
title_full	Micropatterning of planar metal electrodes by vacuum filling microfluidic channel geometries
title_fullStr	Micropatterning of planar metal electrodes by vacuum filling microfluidic channel geometries
title_full_unstemmed	Micropatterning of planar metal electrodes by vacuum filling microfluidic channel geometries
title_sort	micropatterning of planar metal electrodes by vacuum filling microfluidic channel geometries
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/359ed05fc23a4098b1752f2632814f45
work_keys_str_mv	AT stelioschatzimichail micropatterningofplanarmetalelectrodesbyvacuumfillingmicrofluidicchannelgeometries AT pashiinisupramaniam micropatterningofplanarmetalelectrodesbyvacuumfillingmicrofluidicchannelgeometries AT oscarces micropatterningofplanarmetalelectrodesbyvacuumfillingmicrofluidicchannelgeometries AT alisalehireyhani micropatterningofplanarmetalelectrodesbyvacuumfillingmicrofluidicchannelgeometries
_version_	1718387866404388864

Micropatterning of planar metal electrodes by vacuum filling microfluidic channel geometries

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