The effects of exercise on cerebellar growth-associated protein 43 and adenylyl cyclase- associated protein 1 gene expression and proteins in diabetic-induced neuropathy and healthy male wistar rats

Background: The effect of exercise on the cerebellum cells in diabetic-induced neuropathy and healthy situations is not clear yet. Growth-associated protein 43 (GAP-43) and adenylyl cyclase-associated protein 1 (CAP-1) proteins can restore nerve cells. This study aimed to investigate the effect of a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shiva Jahani Golbar, Abbas Ali Gaeini, Maryam Koushkie Jahromi
Formato:	article
Lenguaje:	EN
Publicado:	Wolters Kluwer Medknow Publications 2021
Materias:	aerobic exercise cerebellum diabetic neuropathies Medicine R
Acceso en línea:	https://doaj.org/article/359f8fa8a1664f4b85ceaa86500789f7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:359f8fa8a1664f4b85ceaa86500789f7
record_format	dspace
spelling	oai:doaj.org-article:359f8fa8a1664f4b85ceaa86500789f72021-11-12T10:11:33ZThe effects of exercise on cerebellar growth-associated protein 43 and adenylyl cyclase- associated protein 1 gene expression and proteins in diabetic-induced neuropathy and healthy male wistar rats2008-78022008-821310.4103/ijpvm.IJPVM_416_19https://doaj.org/article/359f8fa8a1664f4b85ceaa86500789f72021-01-01T00:00:00Zhttp://www.ijpvmjournal.net/article.asp?issn=2008-7802;year=2021;volume=12;issue=1;spage=137;epage=137;aulast=Golbarhttps://doaj.org/toc/2008-7802https://doaj.org/toc/2008-8213Background: The effect of exercise on the cerebellum cells in diabetic-induced neuropathy and healthy situations is not clear yet. Growth-associated protein 43 (GAP-43) and adenylyl cyclase-associated protein 1 (CAP-1) proteins can restore nerve cells. This study aimed to investigate the effect of aerobic exercise on GAP-43 and CAP-1 and their mRNA in the cerebellar tissue of diabetic-induced neuropathy and healthy Wistar rats. Methods: Around 40 healthy male Wistar rats with a mean weight of 271 ± 11.2 g were divided randomly into four groups; healthy aerobic exercise, diabetic-aerobic exercise, healthy-control, and diabetic-control. The exercise group performed aerobic exercise 5 days per week for 6 weeks. Results: Exercise increased CAP1 protein in the cerebellum tissue of healthy (P = 0.002) and diabetic (P = 0.002) groups compared with matched control groups. The effect of exercise on CAP1 was greater in diabetic compared with the healthy group (P = 0.002). The expression of CAP1 mRNA in the cerebellum was higher in the healthy exercise compared with the healthy control group (P = 0.002) and in the healthy exercise compared with the diabetic exercise group (P = 0.026). GAP43 protein was lower in the healthy exercise compared with the healthy control group (P = 0.002) while it was higher in diabetic exercise compared to the healthy exercise group (P = 0.002). Expression of GAP43 mRNA in the cerebellum was higher in the healthy (P = 0.002) and diabetic (P = 0.002) exercise groups compared to non-exercise matched groups and in the diabetic control group compared with the healthy control group (P = 0.002). Exercise improved latency in diabetic (P = 0.001) and healthy exercise groups (P = 0.02). No significant difference was found in blood glucose between exercise and control groups (P > 0.05). Conclusion: Exercise improved cerebellar functions in healthy and diabetic rats, probably mediating by CAP1 protein, even without changing blood glucose.Shiva Jahani GolbarAbbas Ali GaeiniMaryam Koushkie JahromiWolters Kluwer Medknow Publicationsarticle aerobic exercisecerebellumdiabetic neuropathies MedicineRENInternational Journal of Preventive Medicine, Vol 12, Iss 1, Pp 137-137 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	aerobic exercise cerebellum diabetic neuropathies Medicine R
spellingShingle	aerobic exercise cerebellum diabetic neuropathies Medicine R Shiva Jahani Golbar Abbas Ali Gaeini Maryam Koushkie Jahromi The effects of exercise on cerebellar growth-associated protein 43 and adenylyl cyclase- associated protein 1 gene expression and proteins in diabetic-induced neuropathy and healthy male wistar rats
description	Background: The effect of exercise on the cerebellum cells in diabetic-induced neuropathy and healthy situations is not clear yet. Growth-associated protein 43 (GAP-43) and adenylyl cyclase-associated protein 1 (CAP-1) proteins can restore nerve cells. This study aimed to investigate the effect of aerobic exercise on GAP-43 and CAP-1 and their mRNA in the cerebellar tissue of diabetic-induced neuropathy and healthy Wistar rats. Methods: Around 40 healthy male Wistar rats with a mean weight of 271 ± 11.2 g were divided randomly into four groups; healthy aerobic exercise, diabetic-aerobic exercise, healthy-control, and diabetic-control. The exercise group performed aerobic exercise 5 days per week for 6 weeks. Results: Exercise increased CAP1 protein in the cerebellum tissue of healthy (P = 0.002) and diabetic (P = 0.002) groups compared with matched control groups. The effect of exercise on CAP1 was greater in diabetic compared with the healthy group (P = 0.002). The expression of CAP1 mRNA in the cerebellum was higher in the healthy exercise compared with the healthy control group (P = 0.002) and in the healthy exercise compared with the diabetic exercise group (P = 0.026). GAP43 protein was lower in the healthy exercise compared with the healthy control group (P = 0.002) while it was higher in diabetic exercise compared to the healthy exercise group (P = 0.002). Expression of GAP43 mRNA in the cerebellum was higher in the healthy (P = 0.002) and diabetic (P = 0.002) exercise groups compared to non-exercise matched groups and in the diabetic control group compared with the healthy control group (P = 0.002). Exercise improved latency in diabetic (P = 0.001) and healthy exercise groups (P = 0.02). No significant difference was found in blood glucose between exercise and control groups (P > 0.05). Conclusion: Exercise improved cerebellar functions in healthy and diabetic rats, probably mediating by CAP1 protein, even without changing blood glucose.
format	article
author	Shiva Jahani Golbar Abbas Ali Gaeini Maryam Koushkie Jahromi
author_facet	Shiva Jahani Golbar Abbas Ali Gaeini Maryam Koushkie Jahromi
author_sort	Shiva Jahani Golbar
title	The effects of exercise on cerebellar growth-associated protein 43 and adenylyl cyclase- associated protein 1 gene expression and proteins in diabetic-induced neuropathy and healthy male wistar rats
title_short	The effects of exercise on cerebellar growth-associated protein 43 and adenylyl cyclase- associated protein 1 gene expression and proteins in diabetic-induced neuropathy and healthy male wistar rats
title_full	The effects of exercise on cerebellar growth-associated protein 43 and adenylyl cyclase- associated protein 1 gene expression and proteins in diabetic-induced neuropathy and healthy male wistar rats
title_fullStr	The effects of exercise on cerebellar growth-associated protein 43 and adenylyl cyclase- associated protein 1 gene expression and proteins in diabetic-induced neuropathy and healthy male wistar rats
title_full_unstemmed	The effects of exercise on cerebellar growth-associated protein 43 and adenylyl cyclase- associated protein 1 gene expression and proteins in diabetic-induced neuropathy and healthy male wistar rats
title_sort	effects of exercise on cerebellar growth-associated protein 43 and adenylyl cyclase- associated protein 1 gene expression and proteins in diabetic-induced neuropathy and healthy male wistar rats
publisher	Wolters Kluwer Medknow Publications
publishDate	2021
url	https://doaj.org/article/359f8fa8a1664f4b85ceaa86500789f7
work_keys_str_mv	AT shivajahanigolbar theeffectsofexerciseoncerebellargrowthassociatedprotein43andadenylylcyclaseassociatedprotein1geneexpressionandproteinsindiabeticinducedneuropathyandhealthymalewistarrats AT abbasaligaeini theeffectsofexerciseoncerebellargrowthassociatedprotein43andadenylylcyclaseassociatedprotein1geneexpressionandproteinsindiabeticinducedneuropathyandhealthymalewistarrats AT maryamkoushkiejahromi theeffectsofexerciseoncerebellargrowthassociatedprotein43andadenylylcyclaseassociatedprotein1geneexpressionandproteinsindiabeticinducedneuropathyandhealthymalewistarrats AT shivajahanigolbar effectsofexerciseoncerebellargrowthassociatedprotein43andadenylylcyclaseassociatedprotein1geneexpressionandproteinsindiabeticinducedneuropathyandhealthymalewistarrats AT abbasaligaeini effectsofexerciseoncerebellargrowthassociatedprotein43andadenylylcyclaseassociatedprotein1geneexpressionandproteinsindiabeticinducedneuropathyandhealthymalewistarrats AT maryamkoushkiejahromi effectsofexerciseoncerebellargrowthassociatedprotein43andadenylylcyclaseassociatedprotein1geneexpressionandproteinsindiabeticinducedneuropathyandhealthymalewistarrats
_version_	1718430980671275008

The effects of exercise on cerebellar growth-associated protein 43 and adenylyl cyclase- associated protein 1 gene expression and proteins in diabetic-induced neuropathy and healthy male wistar rats

Ejemplares similares