Metabarcoding of root-associated ectomycorrhizal fungi of Himalayan pindrow fir through morphotyping and Next Generation Sequencing

Abies pindrow (Royle ex D.Don) Royle (Himalayan pindrow fir) is an imperative conifer native to Kashmir Himalaya, where its mono-dominant forests provide vital ecosystem services, besides being dwelling to endangered flora and fauna. However, in the past few decades, these forests have relentlessly...

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Autores principales:	Rezwana Assad, Zafar Ahmad Reshi, Irfan Rashid, Divya Chetan Wali, Iqra Bashir, Iflah Rafiq
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Abies pindrow Ectomycorrhiza Kashmir Himalaya Metabarcoding Morphotypes Restoration Forestry SD1-669.5 Plant ecology QK900-989
Acceso en línea:	https://doaj.org/article/35a9bc47550c48d8b2af2207b7044cb2
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spelling	oai:doaj.org-article:35a9bc47550c48d8b2af2207b7044cb22021-12-04T04:36:16ZMetabarcoding of root-associated ectomycorrhizal fungi of Himalayan pindrow fir through morphotyping and Next Generation Sequencing2666-719310.1016/j.tfp.2021.100153https://doaj.org/article/35a9bc47550c48d8b2af2207b7044cb22021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666719321000923https://doaj.org/toc/2666-7193Abies pindrow (Royle ex D.Don) Royle (Himalayan pindrow fir) is an imperative conifer native to Kashmir Himalaya, where its mono-dominant forests provide vital ecosystem services, besides being dwelling to endangered flora and fauna. However, in the past few decades, these forests have relentlessly undergone unprecedented deforestation and degradation, and consequently, biodiversity and biotic interactions are jeopardized in these ecosystems. Ectomycorrhizal (ECM) fungi of this fir species may prove helpful in the successful restoration of structural organization and functional integrity of these degraded fir forests. Consequently, the present study was undertaken to explore and document the root-associated ectomycorrhizal symbionts of Himalayan pindrow fir through ectomycorrhizal root tip surveys for morphotyping together with Next Generation Sequencing (Illumina MiSeq) of ITS region of fungal nuclear ribosomal DNA. Fine ECM infected root tips were grouped into fourteen distinct morphotypes based on the morphological attributes, with the monopodial pyramidal type being the most abundant morphotype. The molecular characterization revealed high fungal diversity associated with the roots of Himalayan pindrow fir. A total of 2,51,158 reads were obtained, representing 136 OTUs, of which 62 have confirmed ectomycorrhizal status. In addition, fir roots were found to host a large suite of diverse fungal taxa, including saprotrophs, parasites, and pathogens. FUNGuild assigned 9 prominent guilds to the fungi associated with the fir roots, and ectomycorrhizal fungi represented the largest guild. OTUs belonging to division Basidiomycota were more abundant than those belonging to Ascomycota. Inocybe, Russula, Otidea, Sebacina, Chalara, Tomentella, Cenococcum and Wilcoxina were the abundant ECM forming genera. The results of this study can serve as baseline information for future research and pave the way for experimental evaluation of these ectomycorrhizal mutualists for their prospective use in ectomycorrhization and ECM-mediated forest restoration.Rezwana AssadZafar Ahmad ReshiIrfan RashidDivya Chetan WaliIqra BashirIflah RafiqElsevierarticleAbies pindrowEctomycorrhizaKashmir HimalayaMetabarcodingMorphotypesRestorationForestrySD1-669.5Plant ecologyQK900-989ENTrees, Forests and People, Vol 6, Iss , Pp 100153- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Abies pindrow Ectomycorrhiza Kashmir Himalaya Metabarcoding Morphotypes Restoration Forestry SD1-669.5 Plant ecology QK900-989
spellingShingle	Abies pindrow Ectomycorrhiza Kashmir Himalaya Metabarcoding Morphotypes Restoration Forestry SD1-669.5 Plant ecology QK900-989 Rezwana Assad Zafar Ahmad Reshi Irfan Rashid Divya Chetan Wali Iqra Bashir Iflah Rafiq Metabarcoding of root-associated ectomycorrhizal fungi of Himalayan pindrow fir through morphotyping and Next Generation Sequencing
description	Abies pindrow (Royle ex D.Don) Royle (Himalayan pindrow fir) is an imperative conifer native to Kashmir Himalaya, where its mono-dominant forests provide vital ecosystem services, besides being dwelling to endangered flora and fauna. However, in the past few decades, these forests have relentlessly undergone unprecedented deforestation and degradation, and consequently, biodiversity and biotic interactions are jeopardized in these ecosystems. Ectomycorrhizal (ECM) fungi of this fir species may prove helpful in the successful restoration of structural organization and functional integrity of these degraded fir forests. Consequently, the present study was undertaken to explore and document the root-associated ectomycorrhizal symbionts of Himalayan pindrow fir through ectomycorrhizal root tip surveys for morphotyping together with Next Generation Sequencing (Illumina MiSeq) of ITS region of fungal nuclear ribosomal DNA. Fine ECM infected root tips were grouped into fourteen distinct morphotypes based on the morphological attributes, with the monopodial pyramidal type being the most abundant morphotype. The molecular characterization revealed high fungal diversity associated with the roots of Himalayan pindrow fir. A total of 2,51,158 reads were obtained, representing 136 OTUs, of which 62 have confirmed ectomycorrhizal status. In addition, fir roots were found to host a large suite of diverse fungal taxa, including saprotrophs, parasites, and pathogens. FUNGuild assigned 9 prominent guilds to the fungi associated with the fir roots, and ectomycorrhizal fungi represented the largest guild. OTUs belonging to division Basidiomycota were more abundant than those belonging to Ascomycota. Inocybe, Russula, Otidea, Sebacina, Chalara, Tomentella, Cenococcum and Wilcoxina were the abundant ECM forming genera. The results of this study can serve as baseline information for future research and pave the way for experimental evaluation of these ectomycorrhizal mutualists for their prospective use in ectomycorrhization and ECM-mediated forest restoration.
format	article
author	Rezwana Assad Zafar Ahmad Reshi Irfan Rashid Divya Chetan Wali Iqra Bashir Iflah Rafiq
author_facet	Rezwana Assad Zafar Ahmad Reshi Irfan Rashid Divya Chetan Wali Iqra Bashir Iflah Rafiq
author_sort	Rezwana Assad
title	Metabarcoding of root-associated ectomycorrhizal fungi of Himalayan pindrow fir through morphotyping and Next Generation Sequencing
title_short	Metabarcoding of root-associated ectomycorrhizal fungi of Himalayan pindrow fir through morphotyping and Next Generation Sequencing
title_full	Metabarcoding of root-associated ectomycorrhizal fungi of Himalayan pindrow fir through morphotyping and Next Generation Sequencing
title_fullStr	Metabarcoding of root-associated ectomycorrhizal fungi of Himalayan pindrow fir through morphotyping and Next Generation Sequencing
title_full_unstemmed	Metabarcoding of root-associated ectomycorrhizal fungi of Himalayan pindrow fir through morphotyping and Next Generation Sequencing
title_sort	metabarcoding of root-associated ectomycorrhizal fungi of himalayan pindrow fir through morphotyping and next generation sequencing
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/35a9bc47550c48d8b2af2207b7044cb2
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Metabarcoding of root-associated ectomycorrhizal fungi of Himalayan pindrow fir through morphotyping and Next Generation Sequencing

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