New Reference Genes for qRT-PCR Analysis as a Potential Target for Identification of <i>Trichophyton verrucosum</i> in Different Culture Conditions

Dermatophytes are a group of filamentous fungi infecting skin, hair, and nails that raise great diagnostic difficulties. qRT-PCR is a reliable technique for quantifying gene expression with increasingly frequent use in mycological diagnostics. Knowledge of genes and molecular markers with potential...

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Autores principales:	Sebastian Gnat, Dominik Łagowski, Aneta Nowakiewicz, Aleksandra Trościańczyk, Mariusz Dyląg
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	qRT-PCR technique housekeeping genes <i>Trichophyton verrucosum</i> gene expression Medicine R
Acceso en línea:	https://doaj.org/article/35aa5e9e8b2f4008b4f4f7c67f6930f4
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spelling	oai:doaj.org-article:35aa5e9e8b2f4008b4f4f7c67f6930f42021-11-25T18:37:35ZNew Reference Genes for qRT-PCR Analysis as a Potential Target for Identification of <i>Trichophyton verrucosum</i> in Different Culture Conditions10.3390/pathogens101113612076-0817https://doaj.org/article/35aa5e9e8b2f4008b4f4f7c67f6930f42021-10-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1361https://doaj.org/toc/2076-0817Dermatophytes are a group of filamentous fungi infecting skin, hair, and nails that raise great diagnostic difficulties. qRT-PCR is a reliable technique for quantifying gene expression with increasingly frequent use in mycological diagnostics. Knowledge of genes and molecular markers with potential to be used in the identification of dermatophytes is of great importance for the development of this branch of diagnostics. In this article, the suitability of six candidate reference genes (<i>TUBB</i>, <i>ACTB</i>, <i>ADPRF</i>, <i>RPL2</i>, <i>SDHA</i>, and <i>EEF1A1</i>) was investigated for gene expression analysis in the dermatophyte <i>Trichophyton verrucosum</i>, which was cultured in various mycological media that are commonly used in a diagnostic laboratory, i.e., Sabouraud, potato dextrose, and keratin-supplemented MM-Cove. The different culture conditions are extremely important factors for the growth and physiology of dermatophytes. Gene expression stability was evaluated using geNorm, NormFinder, BestKeeper, and RefFinder algorithms. Regarding the stability of expression, <i>SDHA</i> was the most stable housekeeping gene; hence, this gene is recommended for future qRT-PCR studies on <i>T. verrucosum</i> strains. These results allow us to conclude that the <i>SDHA</i> gene can be an additional good candidate as an identification target in the qRT-PCR technique.Sebastian GnatDominik ŁagowskiAneta NowakiewiczAleksandra TrościańczykMariusz DylągMDPI AGarticleqRT-PCR techniquehousekeeping genes<i>Trichophyton verrucosum</i>gene expressionMedicineRENPathogens, Vol 10, Iss 1361, p 1361 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	qRT-PCR technique housekeeping genes <i>Trichophyton verrucosum</i> gene expression Medicine R
spellingShingle	qRT-PCR technique housekeeping genes <i>Trichophyton verrucosum</i> gene expression Medicine R Sebastian Gnat Dominik Łagowski Aneta Nowakiewicz Aleksandra Trościańczyk Mariusz Dyląg New Reference Genes for qRT-PCR Analysis as a Potential Target for Identification of <i>Trichophyton verrucosum</i> in Different Culture Conditions
description	Dermatophytes are a group of filamentous fungi infecting skin, hair, and nails that raise great diagnostic difficulties. qRT-PCR is a reliable technique for quantifying gene expression with increasingly frequent use in mycological diagnostics. Knowledge of genes and molecular markers with potential to be used in the identification of dermatophytes is of great importance for the development of this branch of diagnostics. In this article, the suitability of six candidate reference genes (<i>TUBB</i>, <i>ACTB</i>, <i>ADPRF</i>, <i>RPL2</i>, <i>SDHA</i>, and <i>EEF1A1</i>) was investigated for gene expression analysis in the dermatophyte <i>Trichophyton verrucosum</i>, which was cultured in various mycological media that are commonly used in a diagnostic laboratory, i.e., Sabouraud, potato dextrose, and keratin-supplemented MM-Cove. The different culture conditions are extremely important factors for the growth and physiology of dermatophytes. Gene expression stability was evaluated using geNorm, NormFinder, BestKeeper, and RefFinder algorithms. Regarding the stability of expression, <i>SDHA</i> was the most stable housekeeping gene; hence, this gene is recommended for future qRT-PCR studies on <i>T. verrucosum</i> strains. These results allow us to conclude that the <i>SDHA</i> gene can be an additional good candidate as an identification target in the qRT-PCR technique.
format	article
author	Sebastian Gnat Dominik Łagowski Aneta Nowakiewicz Aleksandra Trościańczyk Mariusz Dyląg
author_facet	Sebastian Gnat Dominik Łagowski Aneta Nowakiewicz Aleksandra Trościańczyk Mariusz Dyląg
author_sort	Sebastian Gnat
title	New Reference Genes for qRT-PCR Analysis as a Potential Target for Identification of <i>Trichophyton verrucosum</i> in Different Culture Conditions
title_short	New Reference Genes for qRT-PCR Analysis as a Potential Target for Identification of <i>Trichophyton verrucosum</i> in Different Culture Conditions
title_full	New Reference Genes for qRT-PCR Analysis as a Potential Target for Identification of <i>Trichophyton verrucosum</i> in Different Culture Conditions
title_fullStr	New Reference Genes for qRT-PCR Analysis as a Potential Target for Identification of <i>Trichophyton verrucosum</i> in Different Culture Conditions
title_full_unstemmed	New Reference Genes for qRT-PCR Analysis as a Potential Target for Identification of <i>Trichophyton verrucosum</i> in Different Culture Conditions
title_sort	new reference genes for qrt-pcr analysis as a potential target for identification of <i>trichophyton verrucosum</i> in different culture conditions
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/35aa5e9e8b2f4008b4f4f7c67f6930f4
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New Reference Genes for qRT-PCR Analysis as a Potential Target for Identification of <i>Trichophyton verrucosum</i> in Different Culture Conditions

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