The effect of sample age on the metabolic information extracted from formalin-fixed and paraffin embedded tissue samples using desorption electrospray ionization mass spectrometry imaging

Background: Metabolites, especially lipids, have been shown to be promising therapeutic targets. In conjugation with genes and proteins they can be used to identify phenotypes of disease and support the development of targeted treatments. The majority of clinically collected tissue samples are store...

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Autores principales:	Olof Gerdur Isberg, Yuchen Xiang, Sigridur Klara Bodvarsdottir, Jon Gunnlaugur Jonasson, Margret Thorsteinsdottir, Zoltan Takats
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Sample age Lipids DESI-MSI FFPE Mass spectrometry imaging Medical technology R855-855.5
Acceso en línea:	https://doaj.org/article/35ac63ed57164e16955e7fc8184ed25b
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spelling	oai:doaj.org-article:35ac63ed57164e16955e7fc8184ed25b2021-11-04T04:44:12ZThe effect of sample age on the metabolic information extracted from formalin-fixed and paraffin embedded tissue samples using desorption electrospray ionization mass spectrometry imaging2667-145X10.1016/j.jmsacl.2021.10.004https://doaj.org/article/35ac63ed57164e16955e7fc8184ed25b2021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2667145X21000249https://doaj.org/toc/2667-145XBackground: Metabolites, especially lipids, have been shown to be promising therapeutic targets. In conjugation with genes and proteins they can be used to identify phenotypes of disease and support the development of targeted treatments. The majority of clinically collected tissue samples are stored in formalin-fixed and paraffin embedded (FFPE) blocks due to their tissue conservation ability and indefinite storage capacity. For metabolic analysis, however, fresh frozen (FF) samples are currently preferred over FFPE samples due to concerns of metabolic information being lost when preparing the samples. With little or no sample preparation, desorption electrospray ionisation mass spectrometry imaging (DESI-MSI) allows for the study of spatial as well as spectral information.Methods: DESI-MSI analysis was performed on FFPE breast cancer tissue microarray samples from 213 patients collected between the years 1935-2013. Logistic regression (LR) models were built to classify samples based on age and FF samples were used for feature validation.Results: LR models developed on the FFPE samples achieved an average classification accuracy of 96% when predicting their age with a 10-year grouping. Closer examination of the metabolic change over time revealed that the mean signal intensities for the lower mass range (100 - 500 m/z) linearly decrease over time, while the mean intensities for the higher mass range (500 - 900 m/z), remained relatively constant.Conclusions: In our samples, which span over 70 years, sample age has a weak yet quantifiable impact on metabolite content in FFPE samples, while the higher mass range is seemingly unaffected. FFPE samples thus provide an alternative avenue for metabolic analysis of lipids.Olof Gerdur IsbergYuchen XiangSigridur Klara BodvarsdottirJon Gunnlaugur JonassonMargret ThorsteinsdottirZoltan TakatsElsevierarticleSample ageLipidsDESI-MSIFFPEMass spectrometry imagingMedical technologyR855-855.5ENJournal of Mass Spectrometry and Advances in the Clinical Lab, Vol 22, Iss , Pp 50-55 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Sample age Lipids DESI-MSI FFPE Mass spectrometry imaging Medical technology R855-855.5
spellingShingle	Sample age Lipids DESI-MSI FFPE Mass spectrometry imaging Medical technology R855-855.5 Olof Gerdur Isberg Yuchen Xiang Sigridur Klara Bodvarsdottir Jon Gunnlaugur Jonasson Margret Thorsteinsdottir Zoltan Takats The effect of sample age on the metabolic information extracted from formalin-fixed and paraffin embedded tissue samples using desorption electrospray ionization mass spectrometry imaging
description	Background: Metabolites, especially lipids, have been shown to be promising therapeutic targets. In conjugation with genes and proteins they can be used to identify phenotypes of disease and support the development of targeted treatments. The majority of clinically collected tissue samples are stored in formalin-fixed and paraffin embedded (FFPE) blocks due to their tissue conservation ability and indefinite storage capacity. For metabolic analysis, however, fresh frozen (FF) samples are currently preferred over FFPE samples due to concerns of metabolic information being lost when preparing the samples. With little or no sample preparation, desorption electrospray ionisation mass spectrometry imaging (DESI-MSI) allows for the study of spatial as well as spectral information.Methods: DESI-MSI analysis was performed on FFPE breast cancer tissue microarray samples from 213 patients collected between the years 1935-2013. Logistic regression (LR) models were built to classify samples based on age and FF samples were used for feature validation.Results: LR models developed on the FFPE samples achieved an average classification accuracy of 96% when predicting their age with a 10-year grouping. Closer examination of the metabolic change over time revealed that the mean signal intensities for the lower mass range (100 - 500 m/z) linearly decrease over time, while the mean intensities for the higher mass range (500 - 900 m/z), remained relatively constant.Conclusions: In our samples, which span over 70 years, sample age has a weak yet quantifiable impact on metabolite content in FFPE samples, while the higher mass range is seemingly unaffected. FFPE samples thus provide an alternative avenue for metabolic analysis of lipids.
format	article
author	Olof Gerdur Isberg Yuchen Xiang Sigridur Klara Bodvarsdottir Jon Gunnlaugur Jonasson Margret Thorsteinsdottir Zoltan Takats
author_facet	Olof Gerdur Isberg Yuchen Xiang Sigridur Klara Bodvarsdottir Jon Gunnlaugur Jonasson Margret Thorsteinsdottir Zoltan Takats
author_sort	Olof Gerdur Isberg
title	The effect of sample age on the metabolic information extracted from formalin-fixed and paraffin embedded tissue samples using desorption electrospray ionization mass spectrometry imaging
title_short	The effect of sample age on the metabolic information extracted from formalin-fixed and paraffin embedded tissue samples using desorption electrospray ionization mass spectrometry imaging
title_full	The effect of sample age on the metabolic information extracted from formalin-fixed and paraffin embedded tissue samples using desorption electrospray ionization mass spectrometry imaging
title_fullStr	The effect of sample age on the metabolic information extracted from formalin-fixed and paraffin embedded tissue samples using desorption electrospray ionization mass spectrometry imaging
title_full_unstemmed	The effect of sample age on the metabolic information extracted from formalin-fixed and paraffin embedded tissue samples using desorption electrospray ionization mass spectrometry imaging
title_sort	effect of sample age on the metabolic information extracted from formalin-fixed and paraffin embedded tissue samples using desorption electrospray ionization mass spectrometry imaging
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/35ac63ed57164e16955e7fc8184ed25b
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The effect of sample age on the metabolic information extracted from formalin-fixed and paraffin embedded tissue samples using desorption electrospray ionization mass spectrometry imaging

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