Upregulation of signal transducer and activator of transcription 3 in dogs with chronic inflammatory enteropathies

Abstract Background In inflammatory bowel disease (IBD) in humans, phosphorylated signal transducer and activator of transcription 3 (pSTAT3) is upregulated in mucosal epithelial cells and correlates with clinical severity. Hypothesis/Objective To investigate the expression pattern of pSTAT3 in the...

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Autores principales:	Anita Manz, Karin Allenspach, Stefan Kummer, Barbara Richter, Ingrid Walter, Sabine Macho‐Maschler, Alexander Tichy, Iwan A Burgener, Nicole Luckschander‐Zeller
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	canine chronic inflammatory enteropathy IBD STAT3 Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/35af696ad3974bba94f9d156acbd48d6
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spelling	oai:doaj.org-article:35af696ad3974bba94f9d156acbd48d62021-11-30T17:01:03ZUpregulation of signal transducer and activator of transcription 3 in dogs with chronic inflammatory enteropathies1939-16760891-664010.1111/jvim.16141https://doaj.org/article/35af696ad3974bba94f9d156acbd48d62021-05-01T00:00:00Zhttps://doi.org/10.1111/jvim.16141https://doaj.org/toc/0891-6640https://doaj.org/toc/1939-1676Abstract Background In inflammatory bowel disease (IBD) in humans, phosphorylated signal transducer and activator of transcription 3 (pSTAT3) is upregulated in mucosal epithelial cells and correlates with clinical severity. Hypothesis/Objective To investigate the expression pattern of pSTAT3 in the mucosa of dogs with chronic inflammatory enteropathy (CIE) and explore correlations between its expression and clinical and histopathological severity scoring. Animals Twenty‐eight canine CIE patients grouped into food‐responsive enteropathy (FRE; 9), steroid‐responsive enteropathy (SRE; 10), and protein‐losing enteropathy (PLE; 9). Ten healthy beagle dogs served as controls (CO). Methods Retrospective case control study. Immunohistochemistry was used to detect pSTAT3 in canine duodenal mucosa samples. Results Compared to CO, SRE (P < .001) and PLE (P < .001) dogs had significantly higher pSTAT3 expression in the villus epithelium. The SRE group had a significantly higher expression in the villus lamina propria (VLP) compared to controls (P = .009). In the crypt epithelium (CE), all CIE dogs had significantly higher pSTAT3 expression (FRE, P = .002; SRE, P = .003; PLE, P < .001) compared to CO. In the lamina propria crypt region (CLP), dogs with FRE (P = .04) and SRE (P = .03) had significantly upregulated pSTAT3 compared to controls. A positive correlation was found between canine chronic enteropathy clinical activity index (CCECAI) scoring and pSTAT3 expression for both epithelial (rho = .541; P < .001) and crypt regions (rho = .32; P = .02). Conclusions and Clinical Importance pSTAT3 is upregulated in CIE in dogs, correlates with clinical severity, and may be helpful as a clinical marker in dogs with CIE.Anita ManzKarin AllenspachStefan KummerBarbara RichterIngrid WalterSabine Macho‐MaschlerAlexander TichyIwan A BurgenerNicole Luckschander‐ZellerWileyarticlecaninechronic inflammatory enteropathyIBDSTAT3Veterinary medicineSF600-1100ENJournal of Veterinary Internal Medicine, Vol 35, Iss 3, Pp 1288-1296 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	canine chronic inflammatory enteropathy IBD STAT3 Veterinary medicine SF600-1100
spellingShingle	canine chronic inflammatory enteropathy IBD STAT3 Veterinary medicine SF600-1100 Anita Manz Karin Allenspach Stefan Kummer Barbara Richter Ingrid Walter Sabine Macho‐Maschler Alexander Tichy Iwan A Burgener Nicole Luckschander‐Zeller Upregulation of signal transducer and activator of transcription 3 in dogs with chronic inflammatory enteropathies
description	Abstract Background In inflammatory bowel disease (IBD) in humans, phosphorylated signal transducer and activator of transcription 3 (pSTAT3) is upregulated in mucosal epithelial cells and correlates with clinical severity. Hypothesis/Objective To investigate the expression pattern of pSTAT3 in the mucosa of dogs with chronic inflammatory enteropathy (CIE) and explore correlations between its expression and clinical and histopathological severity scoring. Animals Twenty‐eight canine CIE patients grouped into food‐responsive enteropathy (FRE; 9), steroid‐responsive enteropathy (SRE; 10), and protein‐losing enteropathy (PLE; 9). Ten healthy beagle dogs served as controls (CO). Methods Retrospective case control study. Immunohistochemistry was used to detect pSTAT3 in canine duodenal mucosa samples. Results Compared to CO, SRE (P < .001) and PLE (P < .001) dogs had significantly higher pSTAT3 expression in the villus epithelium. The SRE group had a significantly higher expression in the villus lamina propria (VLP) compared to controls (P = .009). In the crypt epithelium (CE), all CIE dogs had significantly higher pSTAT3 expression (FRE, P = .002; SRE, P = .003; PLE, P < .001) compared to CO. In the lamina propria crypt region (CLP), dogs with FRE (P = .04) and SRE (P = .03) had significantly upregulated pSTAT3 compared to controls. A positive correlation was found between canine chronic enteropathy clinical activity index (CCECAI) scoring and pSTAT3 expression for both epithelial (rho = .541; P < .001) and crypt regions (rho = .32; P = .02). Conclusions and Clinical Importance pSTAT3 is upregulated in CIE in dogs, correlates with clinical severity, and may be helpful as a clinical marker in dogs with CIE.
format	article
author	Anita Manz Karin Allenspach Stefan Kummer Barbara Richter Ingrid Walter Sabine Macho‐Maschler Alexander Tichy Iwan A Burgener Nicole Luckschander‐Zeller
author_facet	Anita Manz Karin Allenspach Stefan Kummer Barbara Richter Ingrid Walter Sabine Macho‐Maschler Alexander Tichy Iwan A Burgener Nicole Luckschander‐Zeller
author_sort	Anita Manz
title	Upregulation of signal transducer and activator of transcription 3 in dogs with chronic inflammatory enteropathies
title_short	Upregulation of signal transducer and activator of transcription 3 in dogs with chronic inflammatory enteropathies
title_full	Upregulation of signal transducer and activator of transcription 3 in dogs with chronic inflammatory enteropathies
title_fullStr	Upregulation of signal transducer and activator of transcription 3 in dogs with chronic inflammatory enteropathies
title_full_unstemmed	Upregulation of signal transducer and activator of transcription 3 in dogs with chronic inflammatory enteropathies
title_sort	upregulation of signal transducer and activator of transcription 3 in dogs with chronic inflammatory enteropathies
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/35af696ad3974bba94f9d156acbd48d6
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_version_	1718406437126799360

Upregulation of signal transducer and activator of transcription 3 in dogs with chronic inflammatory enteropathies

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