IS-98-ST1 West Nile virus derived from an infectious cDNA clone retains neuroinvasiveness and neurovirulence properties of the original virus.

Infectious clones of West Nile virus (WNV) have previously been generated and used to decipher the role of viral proteins in WNV virulence. The majority of molecular clones obtained to date have been derived from North American, Australian, or African isolates. Here, we describe the construction of...

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Autores principales:	Céline Bahuon, Philippe Desprès, Nathalie Pardigon, Jean-Jacques Panthier, Nathalie Cordonnier, Steeve Lowenski, Jennifer Richardson, Stéphan Zientara, Sylvie Lecollinet
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/35b0a6c353884eefa4a44c9e354d119d
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spelling	oai:doaj.org-article:35b0a6c353884eefa4a44c9e354d119d2021-11-18T08:11:22ZIS-98-ST1 West Nile virus derived from an infectious cDNA clone retains neuroinvasiveness and neurovirulence properties of the original virus.1932-620310.1371/journal.pone.0047666https://doaj.org/article/35b0a6c353884eefa4a44c9e354d119d2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23110088/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Infectious clones of West Nile virus (WNV) have previously been generated and used to decipher the role of viral proteins in WNV virulence. The majority of molecular clones obtained to date have been derived from North American, Australian, or African isolates. Here, we describe the construction of an infectious cDNA clone of a Mediterranean WNV strain, IS-98-ST1. We characterized the biological properties of the recovered recombinant virus in cell culture and in mice. The growth kinetics of recombinant and parental WNV were similar in Vero cells. Moreover, the phenotype of recombinant and parental WNV was indistinguishable as regards viremia, viral load in the brain, and mortality in susceptible and resistant mice. Finally, the pathobiology of the infectious clone was examined in embryonated chicken eggs. The capacity of different WNV strains to replicate in embryonated chicken eggs closely paralleled their ability to replicate in mice, suggesting that inoculation of embryonated chicken eggs could provide a practical in vivo model for the study of WNV pathogenesis. In conclusion, the IS-98-ST1 infectious clone will allow assessment of the impact of selected mutations and novel genomic changes appearing in emerging European strains pathogenicity and endemic or epidemic potential. This will be invaluable in the context of an increasing number of outbreaks and enhanced severity of infections in the Mediterranean basin and Eastern Europe.Céline BahuonPhilippe DesprèsNathalie PardigonJean-Jacques PanthierNathalie CordonnierSteeve LowenskiJennifer RichardsonStéphan ZientaraSylvie LecollinetPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e47666 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Céline Bahuon Philippe Desprès Nathalie Pardigon Jean-Jacques Panthier Nathalie Cordonnier Steeve Lowenski Jennifer Richardson Stéphan Zientara Sylvie Lecollinet IS-98-ST1 West Nile virus derived from an infectious cDNA clone retains neuroinvasiveness and neurovirulence properties of the original virus.
description	Infectious clones of West Nile virus (WNV) have previously been generated and used to decipher the role of viral proteins in WNV virulence. The majority of molecular clones obtained to date have been derived from North American, Australian, or African isolates. Here, we describe the construction of an infectious cDNA clone of a Mediterranean WNV strain, IS-98-ST1. We characterized the biological properties of the recovered recombinant virus in cell culture and in mice. The growth kinetics of recombinant and parental WNV were similar in Vero cells. Moreover, the phenotype of recombinant and parental WNV was indistinguishable as regards viremia, viral load in the brain, and mortality in susceptible and resistant mice. Finally, the pathobiology of the infectious clone was examined in embryonated chicken eggs. The capacity of different WNV strains to replicate in embryonated chicken eggs closely paralleled their ability to replicate in mice, suggesting that inoculation of embryonated chicken eggs could provide a practical in vivo model for the study of WNV pathogenesis. In conclusion, the IS-98-ST1 infectious clone will allow assessment of the impact of selected mutations and novel genomic changes appearing in emerging European strains pathogenicity and endemic or epidemic potential. This will be invaluable in the context of an increasing number of outbreaks and enhanced severity of infections in the Mediterranean basin and Eastern Europe.
format	article
author	Céline Bahuon Philippe Desprès Nathalie Pardigon Jean-Jacques Panthier Nathalie Cordonnier Steeve Lowenski Jennifer Richardson Stéphan Zientara Sylvie Lecollinet
author_facet	Céline Bahuon Philippe Desprès Nathalie Pardigon Jean-Jacques Panthier Nathalie Cordonnier Steeve Lowenski Jennifer Richardson Stéphan Zientara Sylvie Lecollinet
author_sort	Céline Bahuon
title	IS-98-ST1 West Nile virus derived from an infectious cDNA clone retains neuroinvasiveness and neurovirulence properties of the original virus.
title_short	IS-98-ST1 West Nile virus derived from an infectious cDNA clone retains neuroinvasiveness and neurovirulence properties of the original virus.
title_full	IS-98-ST1 West Nile virus derived from an infectious cDNA clone retains neuroinvasiveness and neurovirulence properties of the original virus.
title_fullStr	IS-98-ST1 West Nile virus derived from an infectious cDNA clone retains neuroinvasiveness and neurovirulence properties of the original virus.
title_full_unstemmed	IS-98-ST1 West Nile virus derived from an infectious cDNA clone retains neuroinvasiveness and neurovirulence properties of the original virus.
title_sort	is-98-st1 west nile virus derived from an infectious cdna clone retains neuroinvasiveness and neurovirulence properties of the original virus.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/35b0a6c353884eefa4a44c9e354d119d
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IS-98-ST1 West Nile virus derived from an infectious cDNA clone retains neuroinvasiveness and neurovirulence properties of the original virus.

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