The cytotoxicity and apoptotic effects of verbascoside on breast cancer 4T1 cell line

Abstract Background Despite significant advancements in breast cancer therapy, novel drugs with lower side effects are still being demanded. In this regard, we investigated the anti-cancer features of verbascoside in 4 T1 mouse mammary tumor cell. Methods First, MTT assay was performed with various...

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Autores principales: Atena Daneshforouz, Samad Nazemi, Omid Gholami, Marzieh Kafami, Bahareh Amin
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Publicado: BMC 2021
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spelling oai:doaj.org-article:35b57539c578433bb6e90579e2da468c2021-12-05T12:11:09ZThe cytotoxicity and apoptotic effects of verbascoside on breast cancer 4T1 cell line10.1186/s40360-021-00540-82050-6511https://doaj.org/article/35b57539c578433bb6e90579e2da468c2021-11-01T00:00:00Zhttps://doi.org/10.1186/s40360-021-00540-8https://doaj.org/toc/2050-6511Abstract Background Despite significant advancements in breast cancer therapy, novel drugs with lower side effects are still being demanded. In this regard, we investigated the anti-cancer features of verbascoside in 4 T1 mouse mammary tumor cell. Methods First, MTT assay was performed with various concentrations (ranging between 5 to 200 μM) of verbascoside and IC50 was calculated. Then the expression of Bax, Bcl-2, and caspase-3 was evaluated in treated 4 T1 cells. In addition, we investigated the expression of TLR4, MyD88, and NF-κB to ascertain the underlying mechanism of the anti-proliferative feature of verbascoside. Also, flow cytometry followed by double PI and Annexin V was conducted to confirm the apoptosis-inducing effect of verbascoside. Results Our results from MTT assay showed verbascoside inhibits proliferation of 4 T1 cancer cells (IC50 117 μM) while is safe for normal HEK293T cells. By qRT-PCR, we observed that verbascoside treatment (100, 117 and, 130 μM) increases the expression of caspase-3 and Bax while reduces the expression of Bcl-2. Also, verbascoside (100, 117 and, 130 μM) increased the expression of TLR4 only at 130 μM dose and the expression of MyD88 whereas reduced the expression of NF-κB at mRNA level. Flow cytometry analysis also confirmed verbascoside induces apoptosis in 4 T1 cells at 117 μM. Conclusion Taken together, our data showed verbascoside is a safe natural compound for normal cells while has apoptosis-inducing feature through TLR4 axis on 4 T1 cells.Atena DaneshforouzSamad NazemiOmid GholamiMarzieh KafamiBahareh AminBMCarticleVerbascosideBreast cancerApoptosisToll like receptorMyD88NF-κBTherapeutics. PharmacologyRM1-950Toxicology. PoisonsRA1190-1270ENBMC Pharmacology and Toxicology, Vol 22, Iss 1, Pp 1-9 (2021)
institution DOAJ
collection DOAJ
language EN
topic Verbascoside
Breast cancer
Apoptosis
Toll like receptor
MyD88
NF-κB
Therapeutics. Pharmacology
RM1-950
Toxicology. Poisons
RA1190-1270
spellingShingle Verbascoside
Breast cancer
Apoptosis
Toll like receptor
MyD88
NF-κB
Therapeutics. Pharmacology
RM1-950
Toxicology. Poisons
RA1190-1270
Atena Daneshforouz
Samad Nazemi
Omid Gholami
Marzieh Kafami
Bahareh Amin
The cytotoxicity and apoptotic effects of verbascoside on breast cancer 4T1 cell line
description Abstract Background Despite significant advancements in breast cancer therapy, novel drugs with lower side effects are still being demanded. In this regard, we investigated the anti-cancer features of verbascoside in 4 T1 mouse mammary tumor cell. Methods First, MTT assay was performed with various concentrations (ranging between 5 to 200 μM) of verbascoside and IC50 was calculated. Then the expression of Bax, Bcl-2, and caspase-3 was evaluated in treated 4 T1 cells. In addition, we investigated the expression of TLR4, MyD88, and NF-κB to ascertain the underlying mechanism of the anti-proliferative feature of verbascoside. Also, flow cytometry followed by double PI and Annexin V was conducted to confirm the apoptosis-inducing effect of verbascoside. Results Our results from MTT assay showed verbascoside inhibits proliferation of 4 T1 cancer cells (IC50 117 μM) while is safe for normal HEK293T cells. By qRT-PCR, we observed that verbascoside treatment (100, 117 and, 130 μM) increases the expression of caspase-3 and Bax while reduces the expression of Bcl-2. Also, verbascoside (100, 117 and, 130 μM) increased the expression of TLR4 only at 130 μM dose and the expression of MyD88 whereas reduced the expression of NF-κB at mRNA level. Flow cytometry analysis also confirmed verbascoside induces apoptosis in 4 T1 cells at 117 μM. Conclusion Taken together, our data showed verbascoside is a safe natural compound for normal cells while has apoptosis-inducing feature through TLR4 axis on 4 T1 cells.
format article
author Atena Daneshforouz
Samad Nazemi
Omid Gholami
Marzieh Kafami
Bahareh Amin
author_facet Atena Daneshforouz
Samad Nazemi
Omid Gholami
Marzieh Kafami
Bahareh Amin
author_sort Atena Daneshforouz
title The cytotoxicity and apoptotic effects of verbascoside on breast cancer 4T1 cell line
title_short The cytotoxicity and apoptotic effects of verbascoside on breast cancer 4T1 cell line
title_full The cytotoxicity and apoptotic effects of verbascoside on breast cancer 4T1 cell line
title_fullStr The cytotoxicity and apoptotic effects of verbascoside on breast cancer 4T1 cell line
title_full_unstemmed The cytotoxicity and apoptotic effects of verbascoside on breast cancer 4T1 cell line
title_sort cytotoxicity and apoptotic effects of verbascoside on breast cancer 4t1 cell line
publisher BMC
publishDate 2021
url https://doaj.org/article/35b57539c578433bb6e90579e2da468c
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