Spent Pleurotus ostreatus Substrate Has Potential for Managing Fusarium Wilt of Banana

A range of basidiomycetes including the edible mushroom Pleurotus ostreatus (Po) can suppress plant pathogens such as Fusarium spp. With the current increase in production and consumption of Po in Uganda, the spent ...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Walter Ocimati, Evans Were, Anthony Fredrick Tazuba, Miguel Dita, Si-Jun Zheng, Guy Blomme
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	basidiomycetes biocontrol corm damage <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> metabolites spent mushroom substrates Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/35c2211c1ce0403ea3616f8bd841eb91
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:35c2211c1ce0403ea3616f8bd841eb91
record_format	dspace
spelling	oai:doaj.org-article:35c2211c1ce0403ea3616f8bd841eb912021-11-25T18:06:02ZSpent <i>Pleurotus ostreatus</i> Substrate Has Potential for Managing Fusarium Wilt of Banana10.3390/jof71109462309-608Xhttps://doaj.org/article/35c2211c1ce0403ea3616f8bd841eb912021-11-01T00:00:00Zhttps://www.mdpi.com/2309-608X/7/11/946https://doaj.org/toc/2309-608XA range of basidiomycetes including the edible mushroom <i>Pleurotus ostreatus</i> (<i>Po</i>) can suppress plant pathogens such as <i>Fusarium</i> spp. With the current increase in production and consumption of <i>Po</i> in Uganda, the spent <i>Po</i> substrate (SPoS) could be an alternative to manage Fusarium wilt of banana (FWB), caused by the soil borne pathogen <i>Fusarium oxysporum</i> f. sp. <i>cubense,</i> race 1 (<i>Foc</i>). This study determined the potential of SPoS to inhibit <i>Foc</i> in vitro and in potted plants. In vitro studies confirmed suppression of <i>Foc</i> in pure co-culture (<i>Po</i> vs. <i>Foc</i>) assays and media amended with different concentrations (0% to 50% <i>w</i>/<i>v</i>) of un-sterilized SPoS filtrates. <i>Foc</i> growth in the sterile SPoS filtrate was comparable to the water control, suggesting possible roles of biotic or thermolabile components of the SPoS. To further verify the suppressive effects of SPoS, pot experiments were carried out with a resistant (‘Mbwazirume’, AAA) and susceptible (‘Sukali Ndizi’, AAB) banana cultivar using both artificially and naturally infested soils. Independent of the inoculation method, SPoS significantly reduced the severity of FWB in pot experiments. Susceptible cultivar ‘Sukali Ndizi’ growing in substrates amended with SPoS showed lower (1.25) corm damage (Scale 0–5) than the un-amended control (3.75). No corm damage was observed in uninoculated controls. The resistant cultivar ‘Mbwazirume’, showed slight (0.25) corm damage only in the Foc-inoculated plants without SPoS. These findings suggest that SPoS could be used as part of the management practices to reduce the impact of FWB.Walter OcimatiEvans WereAnthony Fredrick TazubaMiguel DitaSi-Jun ZhengGuy BlommeMDPI AGarticlebasidiomycetesbiocontrolcorm damage<i>Fusarium oxysporum</i> f. sp. <i>cubense</i>metabolitesspent mushroom substratesBiology (General)QH301-705.5ENJournal of Fungi, Vol 7, Iss 946, p 946 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	basidiomycetes biocontrol corm damage <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> metabolites spent mushroom substrates Biology (General) QH301-705.5
spellingShingle	basidiomycetes biocontrol corm damage <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> metabolites spent mushroom substrates Biology (General) QH301-705.5 Walter Ocimati Evans Were Anthony Fredrick Tazuba Miguel Dita Si-Jun Zheng Guy Blomme Spent <i>Pleurotus ostreatus</i> Substrate Has Potential for Managing Fusarium Wilt of Banana
description	A range of basidiomycetes including the edible mushroom <i>Pleurotus ostreatus</i> (<i>Po</i>) can suppress plant pathogens such as <i>Fusarium</i> spp. With the current increase in production and consumption of <i>Po</i> in Uganda, the spent <i>Po</i> substrate (SPoS) could be an alternative to manage Fusarium wilt of banana (FWB), caused by the soil borne pathogen <i>Fusarium oxysporum</i> f. sp. <i>cubense,</i> race 1 (<i>Foc</i>). This study determined the potential of SPoS to inhibit <i>Foc</i> in vitro and in potted plants. In vitro studies confirmed suppression of <i>Foc</i> in pure co-culture (<i>Po</i> vs. <i>Foc</i>) assays and media amended with different concentrations (0% to 50% <i>w</i>/<i>v</i>) of un-sterilized SPoS filtrates. <i>Foc</i> growth in the sterile SPoS filtrate was comparable to the water control, suggesting possible roles of biotic or thermolabile components of the SPoS. To further verify the suppressive effects of SPoS, pot experiments were carried out with a resistant (‘Mbwazirume’, AAA) and susceptible (‘Sukali Ndizi’, AAB) banana cultivar using both artificially and naturally infested soils. Independent of the inoculation method, SPoS significantly reduced the severity of FWB in pot experiments. Susceptible cultivar ‘Sukali Ndizi’ growing in substrates amended with SPoS showed lower (1.25) corm damage (Scale 0–5) than the un-amended control (3.75). No corm damage was observed in uninoculated controls. The resistant cultivar ‘Mbwazirume’, showed slight (0.25) corm damage only in the Foc-inoculated plants without SPoS. These findings suggest that SPoS could be used as part of the management practices to reduce the impact of FWB.
format	article
author	Walter Ocimati Evans Were Anthony Fredrick Tazuba Miguel Dita Si-Jun Zheng Guy Blomme
author_facet	Walter Ocimati Evans Were Anthony Fredrick Tazuba Miguel Dita Si-Jun Zheng Guy Blomme
author_sort	Walter Ocimati
title	Spent <i>Pleurotus ostreatus</i> Substrate Has Potential for Managing Fusarium Wilt of Banana
title_short	Spent <i>Pleurotus ostreatus</i> Substrate Has Potential for Managing Fusarium Wilt of Banana
title_full	Spent <i>Pleurotus ostreatus</i> Substrate Has Potential for Managing Fusarium Wilt of Banana
title_fullStr	Spent <i>Pleurotus ostreatus</i> Substrate Has Potential for Managing Fusarium Wilt of Banana
title_full_unstemmed	Spent <i>Pleurotus ostreatus</i> Substrate Has Potential for Managing Fusarium Wilt of Banana
title_sort	spent <i>pleurotus ostreatus</i> substrate has potential for managing fusarium wilt of banana
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/35c2211c1ce0403ea3616f8bd841eb91
work_keys_str_mv	AT walterocimati spentipleurotusostreatusisubstratehaspotentialformanagingfusariumwiltofbanana AT evanswere spentipleurotusostreatusisubstratehaspotentialformanagingfusariumwiltofbanana AT anthonyfredricktazuba spentipleurotusostreatusisubstratehaspotentialformanagingfusariumwiltofbanana AT migueldita spentipleurotusostreatusisubstratehaspotentialformanagingfusariumwiltofbanana AT sijunzheng spentipleurotusostreatusisubstratehaspotentialformanagingfusariumwiltofbanana AT guyblomme spentipleurotusostreatusisubstratehaspotentialformanagingfusariumwiltofbanana
_version_	1718411609774227456

Spent <i>Pleurotus ostreatus</i> Substrate Has Potential for Managing Fusarium Wilt of Banana

Ejemplares similares