Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences

The surface structure of the titanium dental implants can modulate the activity of mesenchymal stem cells in order to promote the upregulation of osteoblastic related genes and the release of extracellular matrix (ECM) components. The present work was focused on the in vitro evaluation of the intera...

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Autores principales:	Guya Diletta Marconi, Luigia Fonticoli, Ylenia Della Rocca, Stefano Oliva, Thangavelu Soundara Rajan, Oriana Trubiani, Giovanna Murmura, Francesca Diomede, Jacopo Pizzicannella
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	osseointegration osteogenesis cellular adhesion gene expression extracellular matrix (ECM) Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/35c7a7a0787543e994a7a35543ada295
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spelling	oai:doaj.org-article:35c7a7a0787543e994a7a35543ada2952021-11-25T16:51:06ZEnhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences10.3390/biomedicines91117102227-9059https://doaj.org/article/35c7a7a0787543e994a7a35543ada2952021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1710https://doaj.org/toc/2227-9059The surface structure of the titanium dental implants can modulate the activity of mesenchymal stem cells in order to promote the upregulation of osteoblastic related genes and the release of extracellular matrix (ECM) components. The present work was focused on the in vitro evaluation of the interaction of human periodontal ligament stem cells (hPDLSCs) and two different implant titanium surfaces topography (CTRL and TEST). This study was aimed at analyzing the cytotoxicity of the dental implant surfaces, the cellular adhesion capacity, and the improvement in the release of ECM molecules in an in vitro model. These parameters were carried out by means of the microscopic evaluation, viability assays, immunofluorescence, Western blot and RT-PCR investigations. The knowledge of the cell/implant interaction is essential for implant healing in order to obtain a more performing surfaces that promote the ECM release and provide the starting point to initiate the osseointegration process.Guya Diletta MarconiLuigia FonticoliYlenia Della RoccaStefano OlivaThangavelu Soundara RajanOriana TrubianiGiovanna MurmuraFrancesca DiomedeJacopo PizzicannellaMDPI AGarticleosseointegrationosteogenesiscellular adhesiongene expressionextracellular matrix (ECM)Biology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1710, p 1710 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	osseointegration osteogenesis cellular adhesion gene expression extracellular matrix (ECM) Biology (General) QH301-705.5
spellingShingle	osseointegration osteogenesis cellular adhesion gene expression extracellular matrix (ECM) Biology (General) QH301-705.5 Guya Diletta Marconi Luigia Fonticoli Ylenia Della Rocca Stefano Oliva Thangavelu Soundara Rajan Oriana Trubiani Giovanna Murmura Francesca Diomede Jacopo Pizzicannella Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences
description	The surface structure of the titanium dental implants can modulate the activity of mesenchymal stem cells in order to promote the upregulation of osteoblastic related genes and the release of extracellular matrix (ECM) components. The present work was focused on the in vitro evaluation of the interaction of human periodontal ligament stem cells (hPDLSCs) and two different implant titanium surfaces topography (CTRL and TEST). This study was aimed at analyzing the cytotoxicity of the dental implant surfaces, the cellular adhesion capacity, and the improvement in the release of ECM molecules in an in vitro model. These parameters were carried out by means of the microscopic evaluation, viability assays, immunofluorescence, Western blot and RT-PCR investigations. The knowledge of the cell/implant interaction is essential for implant healing in order to obtain a more performing surfaces that promote the ECM release and provide the starting point to initiate the osseointegration process.
format	article
author	Guya Diletta Marconi Luigia Fonticoli Ylenia Della Rocca Stefano Oliva Thangavelu Soundara Rajan Oriana Trubiani Giovanna Murmura Francesca Diomede Jacopo Pizzicannella
author_facet	Guya Diletta Marconi Luigia Fonticoli Ylenia Della Rocca Stefano Oliva Thangavelu Soundara Rajan Oriana Trubiani Giovanna Murmura Francesca Diomede Jacopo Pizzicannella
author_sort	Guya Diletta Marconi
title	Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences
title_short	Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences
title_full	Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences
title_fullStr	Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences
title_full_unstemmed	Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences
title_sort	enhanced extracellular matrix deposition on titanium implant surfaces: cellular and molecular evidences
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/35c7a7a0787543e994a7a35543ada295
work_keys_str_mv	AT guyadilettamarconi enhancedextracellularmatrixdepositionontitaniumimplantsurfacescellularandmolecularevidences AT luigiafonticoli enhancedextracellularmatrixdepositionontitaniumimplantsurfacescellularandmolecularevidences AT yleniadellarocca enhancedextracellularmatrixdepositionontitaniumimplantsurfacescellularandmolecularevidences AT stefanooliva enhancedextracellularmatrixdepositionontitaniumimplantsurfacescellularandmolecularevidences AT thangavelusoundararajan enhancedextracellularmatrixdepositionontitaniumimplantsurfacescellularandmolecularevidences AT orianatrubiani enhancedextracellularmatrixdepositionontitaniumimplantsurfacescellularandmolecularevidences AT giovannamurmura enhancedextracellularmatrixdepositionontitaniumimplantsurfacescellularandmolecularevidences AT francescadiomede enhancedextracellularmatrixdepositionontitaniumimplantsurfacescellularandmolecularevidences AT jacopopizzicannella enhancedextracellularmatrixdepositionontitaniumimplantsurfacescellularandmolecularevidences
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Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences

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