Exosome Derived from Coronary Serum of Patients with Myocardial Infarction Promotes Angiogenesis Through the miRNA-143/IGF-IR Pathway

Tao Geng, Zhi-Yuan Song, Jing-Xian Xing, Bing-Xun Wang, Shi-Peng Dai, Ze-Sheng Xu Department of Cardiovascular Disease, Cangzhou Central Hospital of Tianjin Medical University, Cangzhou, Hebei Province, People’s Republic of ChinaCorrespondence: Ze-Sheng XuDepartment of Cardiovascular Disea...

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Autores principales:	Geng T, Song ZY, Xing JX, Wang BX, Dai SP, Xu ZS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	myocardial ischemia exosome mirna-143 insulin-like growth factor 1 receptor angiogenesis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/35cb3e55943045e88ff3d08c6995283c
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spelling	oai:doaj.org-article:35cb3e55943045e88ff3d08c6995283c2021-12-02T09:53:57ZExosome Derived from Coronary Serum of Patients with Myocardial Infarction Promotes Angiogenesis Through the miRNA-143/IGF-IR Pathway1178-2013https://doaj.org/article/35cb3e55943045e88ff3d08c6995283c2020-04-01T00:00:00Zhttps://www.dovepress.com/exosome-derived-from-coronary-serum-of-patients-with-myocardial-infarc-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Tao Geng, Zhi-Yuan Song, Jing-Xian Xing, Bing-Xun Wang, Shi-Peng Dai, Ze-Sheng Xu Department of Cardiovascular Disease, Cangzhou Central Hospital of Tianjin Medical University, Cangzhou, Hebei Province, People’s Republic of ChinaCorrespondence: Ze-Sheng XuDepartment of Cardiovascular Disease, Cangzhou Central Hospital of Tianjin Medical University, No. 16, Xinhua West Road, Cangzhou, Hebei Province, People’s Republic of ChinaTel +86-15003172868Email xutuqian848141@163.comPurpose: Myocardial ischemia-reperfusion injury primarily causes myocardial infarction (MI), which is manifested by cell death. Angiogenesis is essential for repair and regeneration in cardiac tissue after MI. In this study, we aimed to investigate the effect of exosomes derived from the serum of MI patients in angiogenesis and its related mechanism.Patients and Methods: Exosomes, isolated from serum, were collected from MI (MI-exosome) and control (Con-exosome) patients. After coculturing with human umbilical vein endothelial cells, MI-exosome promoted cell proliferation, migration, and tube formation.Results: The results revealed that the production and release of MI-exosome were associated with cardiomyocytes. Moreover, microarray assays demonstrated that miRNA-143 was significantly decreased in MI-exosome. Meanwhile, the overexpression and knockdown of miRNA-143 could inhibit and enhance angiogenesis, respectively. Furthermore, the effect of exosomal miRNA-143 on angiogenesis was mediated by its targeting gene, insulin-like growth factor 1 receptor (IGF-IR), and was associated with the production of nitric oxide (NO).Conclusion: Taken together, exosomes derived from the serum of patients with MI promoted angiogenesis through the IGF-IR/NO signaling pathway. The results provide novel understanding of the function of exosomes in MI.Keywords: myocardial ischemia, exosome, miRNA-143, insulin-like growth factor 1 receptor, angiogenesisGeng TSong ZYXing JXWang BXDai SPXu ZSDove Medical Pressarticlemyocardial ischemiaexosomemirna-143insulin-like growth factor 1 receptorangiogenesisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 2647-2658 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	myocardial ischemia exosome mirna-143 insulin-like growth factor 1 receptor angiogenesis Medicine (General) R5-920
spellingShingle	myocardial ischemia exosome mirna-143 insulin-like growth factor 1 receptor angiogenesis Medicine (General) R5-920 Geng T Song ZY Xing JX Wang BX Dai SP Xu ZS Exosome Derived from Coronary Serum of Patients with Myocardial Infarction Promotes Angiogenesis Through the miRNA-143/IGF-IR Pathway
description	Tao Geng, Zhi-Yuan Song, Jing-Xian Xing, Bing-Xun Wang, Shi-Peng Dai, Ze-Sheng Xu Department of Cardiovascular Disease, Cangzhou Central Hospital of Tianjin Medical University, Cangzhou, Hebei Province, People’s Republic of ChinaCorrespondence: Ze-Sheng XuDepartment of Cardiovascular Disease, Cangzhou Central Hospital of Tianjin Medical University, No. 16, Xinhua West Road, Cangzhou, Hebei Province, People’s Republic of ChinaTel +86-15003172868Email xutuqian848141@163.comPurpose: Myocardial ischemia-reperfusion injury primarily causes myocardial infarction (MI), which is manifested by cell death. Angiogenesis is essential for repair and regeneration in cardiac tissue after MI. In this study, we aimed to investigate the effect of exosomes derived from the serum of MI patients in angiogenesis and its related mechanism.Patients and Methods: Exosomes, isolated from serum, were collected from MI (MI-exosome) and control (Con-exosome) patients. After coculturing with human umbilical vein endothelial cells, MI-exosome promoted cell proliferation, migration, and tube formation.Results: The results revealed that the production and release of MI-exosome were associated with cardiomyocytes. Moreover, microarray assays demonstrated that miRNA-143 was significantly decreased in MI-exosome. Meanwhile, the overexpression and knockdown of miRNA-143 could inhibit and enhance angiogenesis, respectively. Furthermore, the effect of exosomal miRNA-143 on angiogenesis was mediated by its targeting gene, insulin-like growth factor 1 receptor (IGF-IR), and was associated with the production of nitric oxide (NO).Conclusion: Taken together, exosomes derived from the serum of patients with MI promoted angiogenesis through the IGF-IR/NO signaling pathway. The results provide novel understanding of the function of exosomes in MI.Keywords: myocardial ischemia, exosome, miRNA-143, insulin-like growth factor 1 receptor, angiogenesis
format	article
author	Geng T Song ZY Xing JX Wang BX Dai SP Xu ZS
author_facet	Geng T Song ZY Xing JX Wang BX Dai SP Xu ZS
author_sort	Geng T
title	Exosome Derived from Coronary Serum of Patients with Myocardial Infarction Promotes Angiogenesis Through the miRNA-143/IGF-IR Pathway
title_short	Exosome Derived from Coronary Serum of Patients with Myocardial Infarction Promotes Angiogenesis Through the miRNA-143/IGF-IR Pathway
title_full	Exosome Derived from Coronary Serum of Patients with Myocardial Infarction Promotes Angiogenesis Through the miRNA-143/IGF-IR Pathway
title_fullStr	Exosome Derived from Coronary Serum of Patients with Myocardial Infarction Promotes Angiogenesis Through the miRNA-143/IGF-IR Pathway
title_full_unstemmed	Exosome Derived from Coronary Serum of Patients with Myocardial Infarction Promotes Angiogenesis Through the miRNA-143/IGF-IR Pathway
title_sort	exosome derived from coronary serum of patients with myocardial infarction promotes angiogenesis through the mirna-143/igf-ir pathway
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/35cb3e55943045e88ff3d08c6995283c
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Exosome Derived from Coronary Serum of Patients with Myocardial Infarction Promotes Angiogenesis Through the miRNA-143/IGF-IR Pathway

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