Plasma microRNA signature associated with retinopathy in patients with type 2 diabetes

Abstract Diabetic retinopathy (DR) is a leading cause of vision loss and disability. Effective management of DR depends on prompt treatment and would benefit from biomarkers for screening and pre-symptomatic detection of retinopathy in diabetic patients. MicroRNAs (miRNAs) are post-transcriptional r...

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Autores principales:	Donato Santovito, Lisa Toto, Velia De Nardis, Pamela Marcantonio, Rossella D’Aloisio, Alessandra Mastropasqua, Domenico De Cesare, Marco Bucci, Camilla Paganelli, Lucia Natarelli, Christian Weber, Agostino Consoli, Rodolfo Mastropasqua, Francesco Cipollone
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/35cd4b47215748fbab275edf21118cab
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spelling	oai:doaj.org-article:35cd4b47215748fbab275edf21118cab2021-12-02T14:21:58ZPlasma microRNA signature associated with retinopathy in patients with type 2 diabetes10.1038/s41598-021-83047-w2045-2322https://doaj.org/article/35cd4b47215748fbab275edf21118cab2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83047-whttps://doaj.org/toc/2045-2322Abstract Diabetic retinopathy (DR) is a leading cause of vision loss and disability. Effective management of DR depends on prompt treatment and would benefit from biomarkers for screening and pre-symptomatic detection of retinopathy in diabetic patients. MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression which are released in the bloodstream and may serve as biomarkers. Little is known on circulating miRNAs in patients with type 2 diabetes (T2DM) and DR. Here we show that DR is associated with higher circulating miR-25-3p (P = 0.004) and miR-320b (P = 0.011) and lower levels of miR-495-3p (P < 0.001) in a cohort of patients with T2DM with DR (n = 20), compared with diabetic subjects without DR (n = 10) and healthy individuals (n = 10). These associations persisted significant after adjustment for age, gender, and HbA1c. The circulating levels of these miRNAs correlated with severity of the disease and their concomitant evaluation showed high accuracy for identifying DR (AUROC = 0.93; P < 0.001). Gene ontology analysis of validated targets revealed enrichment in pathways such as regulation of metabolic process (P = 1.5 × 10–20), of cell response to stress (P = 1.9 × 10–14), and development of blood vessels (P = 2.7 × 10–14). Pending external validation, we anticipate that these miRNAs may serve as putative disease biomarkers and highlight novel molecular targets for improving care of patients with diabetic retinopathy.Donato SantovitoLisa TotoVelia De NardisPamela MarcantonioRossella D’AloisioAlessandra MastropasquaDomenico De CesareMarco BucciCamilla PaganelliLucia NatarelliChristian WeberAgostino ConsoliRodolfo MastropasquaFrancesco CipolloneNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Donato Santovito Lisa Toto Velia De Nardis Pamela Marcantonio Rossella D’Aloisio Alessandra Mastropasqua Domenico De Cesare Marco Bucci Camilla Paganelli Lucia Natarelli Christian Weber Agostino Consoli Rodolfo Mastropasqua Francesco Cipollone Plasma microRNA signature associated with retinopathy in patients with type 2 diabetes
description	Abstract Diabetic retinopathy (DR) is a leading cause of vision loss and disability. Effective management of DR depends on prompt treatment and would benefit from biomarkers for screening and pre-symptomatic detection of retinopathy in diabetic patients. MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression which are released in the bloodstream and may serve as biomarkers. Little is known on circulating miRNAs in patients with type 2 diabetes (T2DM) and DR. Here we show that DR is associated with higher circulating miR-25-3p (P = 0.004) and miR-320b (P = 0.011) and lower levels of miR-495-3p (P < 0.001) in a cohort of patients with T2DM with DR (n = 20), compared with diabetic subjects without DR (n = 10) and healthy individuals (n = 10). These associations persisted significant after adjustment for age, gender, and HbA1c. The circulating levels of these miRNAs correlated with severity of the disease and their concomitant evaluation showed high accuracy for identifying DR (AUROC = 0.93; P < 0.001). Gene ontology analysis of validated targets revealed enrichment in pathways such as regulation of metabolic process (P = 1.5 × 10–20), of cell response to stress (P = 1.9 × 10–14), and development of blood vessels (P = 2.7 × 10–14). Pending external validation, we anticipate that these miRNAs may serve as putative disease biomarkers and highlight novel molecular targets for improving care of patients with diabetic retinopathy.
format	article
author	Donato Santovito Lisa Toto Velia De Nardis Pamela Marcantonio Rossella D’Aloisio Alessandra Mastropasqua Domenico De Cesare Marco Bucci Camilla Paganelli Lucia Natarelli Christian Weber Agostino Consoli Rodolfo Mastropasqua Francesco Cipollone
author_facet	Donato Santovito Lisa Toto Velia De Nardis Pamela Marcantonio Rossella D’Aloisio Alessandra Mastropasqua Domenico De Cesare Marco Bucci Camilla Paganelli Lucia Natarelli Christian Weber Agostino Consoli Rodolfo Mastropasqua Francesco Cipollone
author_sort	Donato Santovito
title	Plasma microRNA signature associated with retinopathy in patients with type 2 diabetes
title_short	Plasma microRNA signature associated with retinopathy in patients with type 2 diabetes
title_full	Plasma microRNA signature associated with retinopathy in patients with type 2 diabetes
title_fullStr	Plasma microRNA signature associated with retinopathy in patients with type 2 diabetes
title_full_unstemmed	Plasma microRNA signature associated with retinopathy in patients with type 2 diabetes
title_sort	plasma microrna signature associated with retinopathy in patients with type 2 diabetes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/35cd4b47215748fbab275edf21118cab
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Plasma microRNA signature associated with retinopathy in patients with type 2 diabetes

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