Efficicent (R)-phenylethanol production with enantioselectivity-alerted (S)-carbonyl reductase II and NADPH regeneration.
The NADPH-dependent (S)-carbonyl reductaseII from Candida parapsilosis catalyzes acetophenone to chiral phenylethanol in a very low yield of 3.2%. Site-directed mutagenesis was used to design two mutants Ala220Asp and Glu228Ser, inside or adjacent to the substrate-binding pocket. Both mutations caus...
Guardado en:
Autores principales: | Rongzhen Zhang, Botao Zhang, Yan Xu, Yaohui Li, Ming Li, Hongbo Liang, Rong Xiao |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Public Library of Science (PLoS)
2013
|
Materias: | |
Acceso en línea: | https://doaj.org/article/35cf5e245ba14e7393292cf3bc9e3e9d |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
Ejemplares similares
-
Molecular Cloning and Identification of NADPH Cytochrome P450 Reductase from <i>Panax ginseng</i>
por: Xian Zou, et al.
Publicado: (2021) -
Copper oxide-based cathode for direct NADPH regeneration
por: J. T. Kadowaki, et al.
Publicado: (2021) -
Effects of Diminished NADPH:cytochrome P450 Reductase in Human Hepatocytes on Lipid and Bile Acid Homeostasis
por: Tamara Heintze, et al.
Publicado: (2021) -
Effects of aroma quality on the biotransformation of natural 2-phenylethanol produced using ascorbic acid
por: Tian,Xun, et al.
Publicado: (2015) -
Functional status in the COVID-19 era: ALERT, ALERT, ALERT!
por: A. Marques
Publicado: (2021)