The microRNAome of pregnancy: deciphering miRNA networks at the maternal-fetal interface.

MicroRNAs (miRNAs) post-transcriptionally regulate a vast network of genes by inhibiting mRNA translation. Aberrant miRNA expression profiles have been implicated in pathologies and physiological processes including pregnancy and angiogenesis. Using our established model of implantation failure and...

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Autores principales:	Jocelyn M Wessels, Andrew K Edwards, Kasra Khalaj, Rami T Kridli, Mallikarjun Bidarimath, Chandrakant Tayade
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/35e818f0bead4ff28cfd50be1745ed4e
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spelling	oai:doaj.org-article:35e818f0bead4ff28cfd50be1745ed4e2021-11-18T08:45:06ZThe microRNAome of pregnancy: deciphering miRNA networks at the maternal-fetal interface.1932-620310.1371/journal.pone.0072264https://doaj.org/article/35e818f0bead4ff28cfd50be1745ed4e2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24278102/?tool=EBIhttps://doaj.org/toc/1932-6203MicroRNAs (miRNAs) post-transcriptionally regulate a vast network of genes by inhibiting mRNA translation. Aberrant miRNA expression profiles have been implicated in pathologies and physiological processes including pregnancy and angiogenesis. Using our established model of implantation failure and spontaneous fetal loss in pigs (Sus scrofa), 236 miRNAs were profiled and compared between 1) non-pregnant and pregnant endometrium, 2) maternal and fetal tissues, and 3) viable and growth-arrested conceptus attachment sites by microarray and Real-Time PCR. Many significant differences in miRNA expression were observed between each of the aforementioned comparisons, and several were validated by PCR. Results indicated which miRNAs were important during pregnancy, which were elevated on the maternal or fetal side of the maternal-fetal interface, and they implicated the maternal expression of miR-10a, 27a, 29c, 323, 331-5p, 339-3p, 374b-5p, and 935 in the spontaneous loss observed in pigs. Several putative mRNA targets of the miRNAs (elevated in endometrium associated with arresting conceptuses) were assessed by quantitative Real-Time PCR and were depressed, supporting their regulation by miRNAs. Finally, targets were clustered by function to obtain ranked lists of gene networks that indicated which pathways/physiological processes might be important in non-pregnant (extracellular matrix factors) versus pregnant endometrium (nuclear transcription factor regulation), maternal (blood vessel development) versus fetal (neuronal differentiation) tissue, and healthy (extracellular matrix factors) versus arresting (GRAM domain) conceptus attachment sites. Overall, we demonstrate the presence of miRNAs on both sides of the maternal-fetal interface, implicate them in spontaneous fetal loss, and present a unique glimpse into the vast microRNAome of pregnancy.Jocelyn M WesselsAndrew K EdwardsKasra KhalajRami T KridliMallikarjun BidarimathChandrakant TayadePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e72264 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jocelyn M Wessels Andrew K Edwards Kasra Khalaj Rami T Kridli Mallikarjun Bidarimath Chandrakant Tayade The microRNAome of pregnancy: deciphering miRNA networks at the maternal-fetal interface.
description	MicroRNAs (miRNAs) post-transcriptionally regulate a vast network of genes by inhibiting mRNA translation. Aberrant miRNA expression profiles have been implicated in pathologies and physiological processes including pregnancy and angiogenesis. Using our established model of implantation failure and spontaneous fetal loss in pigs (Sus scrofa), 236 miRNAs were profiled and compared between 1) non-pregnant and pregnant endometrium, 2) maternal and fetal tissues, and 3) viable and growth-arrested conceptus attachment sites by microarray and Real-Time PCR. Many significant differences in miRNA expression were observed between each of the aforementioned comparisons, and several were validated by PCR. Results indicated which miRNAs were important during pregnancy, which were elevated on the maternal or fetal side of the maternal-fetal interface, and they implicated the maternal expression of miR-10a, 27a, 29c, 323, 331-5p, 339-3p, 374b-5p, and 935 in the spontaneous loss observed in pigs. Several putative mRNA targets of the miRNAs (elevated in endometrium associated with arresting conceptuses) were assessed by quantitative Real-Time PCR and were depressed, supporting their regulation by miRNAs. Finally, targets were clustered by function to obtain ranked lists of gene networks that indicated which pathways/physiological processes might be important in non-pregnant (extracellular matrix factors) versus pregnant endometrium (nuclear transcription factor regulation), maternal (blood vessel development) versus fetal (neuronal differentiation) tissue, and healthy (extracellular matrix factors) versus arresting (GRAM domain) conceptus attachment sites. Overall, we demonstrate the presence of miRNAs on both sides of the maternal-fetal interface, implicate them in spontaneous fetal loss, and present a unique glimpse into the vast microRNAome of pregnancy.
format	article
author	Jocelyn M Wessels Andrew K Edwards Kasra Khalaj Rami T Kridli Mallikarjun Bidarimath Chandrakant Tayade
author_facet	Jocelyn M Wessels Andrew K Edwards Kasra Khalaj Rami T Kridli Mallikarjun Bidarimath Chandrakant Tayade
author_sort	Jocelyn M Wessels
title	The microRNAome of pregnancy: deciphering miRNA networks at the maternal-fetal interface.
title_short	The microRNAome of pregnancy: deciphering miRNA networks at the maternal-fetal interface.
title_full	The microRNAome of pregnancy: deciphering miRNA networks at the maternal-fetal interface.
title_fullStr	The microRNAome of pregnancy: deciphering miRNA networks at the maternal-fetal interface.
title_full_unstemmed	The microRNAome of pregnancy: deciphering miRNA networks at the maternal-fetal interface.
title_sort	micrornaome of pregnancy: deciphering mirna networks at the maternal-fetal interface.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/35e818f0bead4ff28cfd50be1745ed4e
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The microRNAome of pregnancy: deciphering miRNA networks at the maternal-fetal interface.

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