Image Processing Filters for Grids of Cells Analogous to Filters Processing Grids of Pixels

Intra- and extra-cellular processes shape tissues together. For understanding how neighborhood relationships between cells play a role in this process, having image processing filters based on these relationships would be beneficial. Those operations are known and their application to microscopy ima...

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Autor principal: Robert Haase 
Formato: article
Lenguaje:EN
Publicado: Frontiers Media S.A. 2021
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Acceso en línea:https://doaj.org/article/35e945d83b144979a83dc4410c9559b3
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Sumario:Intra- and extra-cellular processes shape tissues together. For understanding how neighborhood relationships between cells play a role in this process, having image processing filters based on these relationships would be beneficial. Those operations are known and their application to microscopy image data typically requires programming skills. User-friendly general purpose tools for pursuing image processing on a level of neighboring cells were yet missing. In this manuscript I demonstrate image processing filters which process grids of cells on tissue level and the analogy to their better known counter parts processing grids of pixels. The tools are available as part of free and open source software in the ImageJ/Fiji and napari ecosystems and their application does not require any programming experience.