Remifentanil pretreatment ameliorates H/R-induced cardiac microvascular endothelial cell dysfunction by regulating the PI3K/Akt/HIF-1α signaling pathway

Restoration of blood supply through medical or surgical intervention is a commonly adopted method for acute myocardial ischemia, but is also a trigger for cardiac ischemia/reperfusion injury. Studies have shown that remifentanil (REM) displays cardioprotective effects. In this study, the effects of...

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Autores principales:	Xiaojun Li, Zhenping Gui, Huizi Liu, Shaojie Qian, Yanan Jia, Xiaopan Luo
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	remifentanil myocardial ischemia hypoxia/reoxygenation pi3k/akt/hif-1α cardiac microvascular endothelial cells Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/35ebde6cf21047f99e4f05dfd6cba8fb
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spelling	oai:doaj.org-article:35ebde6cf21047f99e4f05dfd6cba8fb2021-11-11T14:23:43ZRemifentanil pretreatment ameliorates H/R-induced cardiac microvascular endothelial cell dysfunction by regulating the PI3K/Akt/HIF-1α signaling pathway2165-59792165-598710.1080/21655979.2021.1969843https://doaj.org/article/35ebde6cf21047f99e4f05dfd6cba8fb2021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1969843https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Restoration of blood supply through medical or surgical intervention is a commonly adopted method for acute myocardial ischemia, but is also a trigger for cardiac ischemia/reperfusion injury. Studies have shown that remifentanil (REM) displays cardioprotective effects. In this study, the effects of REM on HCMEC viability were examined before and after the induction of H/R using Cell Counting Kit-8 assays. Wound healing and Matrigel angiogenesis assays were performed to assess HCMEC migration and angiogenesis, respectively. Commercial kits and western blotting were used to determine the endothelial barrier function of H/R-stimulated HCMECs with or without REM treatment. The expression of PI3K/Akt/hypoxia-inducible factor-1α (HIF-1α) pathway-related proteins was detected by western blotting. After pre-treatment with PI3K/Akt, the effects of REM on H/R-induced HCMEC injury were examined. We found that pre-treatment with REM displayed no impact on HCMEC viability under normal conditions but noticeably improved cell viability following H/R. The migratory abilities and tube-like structure formations of H/R-stimulated HCMECs were both enhanced by REM in a concentration-dependent manner. REM also decreased the permeability of H/R-stimulated HCMECs and upregulated the expression of tight junction proteins. Furthermore REM increased the expression of PI3K/Akt/HIF-1α signaling-related proteins in HCMECs. Inhibition of PI3K/Akt rescued REM-enhanced HCMEC function under H/R condition. Therefore, the present study demonstrated that REM pretreatment ameliorated H/R-induced HCMEC dysfunction by regulating the PI3K/Akt/HIF-1α signaling pathway.Xiaojun LiZhenping GuiHuizi LiuShaojie QianYanan JiaXiaopan LuoTaylor & Francis Grouparticleremifentanilmyocardial ischemiahypoxia/reoxygenationpi3k/akt/hif-1αcardiac microvascular endothelial cellsBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 1, Pp 7872-7881 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	remifentanil myocardial ischemia hypoxia/reoxygenation pi3k/akt/hif-1α cardiac microvascular endothelial cells Biotechnology TP248.13-248.65
spellingShingle	remifentanil myocardial ischemia hypoxia/reoxygenation pi3k/akt/hif-1α cardiac microvascular endothelial cells Biotechnology TP248.13-248.65 Xiaojun Li Zhenping Gui Huizi Liu Shaojie Qian Yanan Jia Xiaopan Luo Remifentanil pretreatment ameliorates H/R-induced cardiac microvascular endothelial cell dysfunction by regulating the PI3K/Akt/HIF-1α signaling pathway
description	Restoration of blood supply through medical or surgical intervention is a commonly adopted method for acute myocardial ischemia, but is also a trigger for cardiac ischemia/reperfusion injury. Studies have shown that remifentanil (REM) displays cardioprotective effects. In this study, the effects of REM on HCMEC viability were examined before and after the induction of H/R using Cell Counting Kit-8 assays. Wound healing and Matrigel angiogenesis assays were performed to assess HCMEC migration and angiogenesis, respectively. Commercial kits and western blotting were used to determine the endothelial barrier function of H/R-stimulated HCMECs with or without REM treatment. The expression of PI3K/Akt/hypoxia-inducible factor-1α (HIF-1α) pathway-related proteins was detected by western blotting. After pre-treatment with PI3K/Akt, the effects of REM on H/R-induced HCMEC injury were examined. We found that pre-treatment with REM displayed no impact on HCMEC viability under normal conditions but noticeably improved cell viability following H/R. The migratory abilities and tube-like structure formations of H/R-stimulated HCMECs were both enhanced by REM in a concentration-dependent manner. REM also decreased the permeability of H/R-stimulated HCMECs and upregulated the expression of tight junction proteins. Furthermore REM increased the expression of PI3K/Akt/HIF-1α signaling-related proteins in HCMECs. Inhibition of PI3K/Akt rescued REM-enhanced HCMEC function under H/R condition. Therefore, the present study demonstrated that REM pretreatment ameliorated H/R-induced HCMEC dysfunction by regulating the PI3K/Akt/HIF-1α signaling pathway.
format	article
author	Xiaojun Li Zhenping Gui Huizi Liu Shaojie Qian Yanan Jia Xiaopan Luo
author_facet	Xiaojun Li Zhenping Gui Huizi Liu Shaojie Qian Yanan Jia Xiaopan Luo
author_sort	Xiaojun Li
title	Remifentanil pretreatment ameliorates H/R-induced cardiac microvascular endothelial cell dysfunction by regulating the PI3K/Akt/HIF-1α signaling pathway
title_short	Remifentanil pretreatment ameliorates H/R-induced cardiac microvascular endothelial cell dysfunction by regulating the PI3K/Akt/HIF-1α signaling pathway
title_full	Remifentanil pretreatment ameliorates H/R-induced cardiac microvascular endothelial cell dysfunction by regulating the PI3K/Akt/HIF-1α signaling pathway
title_fullStr	Remifentanil pretreatment ameliorates H/R-induced cardiac microvascular endothelial cell dysfunction by regulating the PI3K/Akt/HIF-1α signaling pathway
title_full_unstemmed	Remifentanil pretreatment ameliorates H/R-induced cardiac microvascular endothelial cell dysfunction by regulating the PI3K/Akt/HIF-1α signaling pathway
title_sort	remifentanil pretreatment ameliorates h/r-induced cardiac microvascular endothelial cell dysfunction by regulating the pi3k/akt/hif-1α signaling pathway
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/35ebde6cf21047f99e4f05dfd6cba8fb
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Remifentanil pretreatment ameliorates H/R-induced cardiac microvascular endothelial cell dysfunction by regulating the PI3K/Akt/HIF-1α signaling pathway

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