Hanging Drop, A Best Three-Dimensional (3D) Culture Method for Primary Buffalo and Sheep Hepatocytes

Abstract Livestock, having close resemblance to humans, could be a better source of primary hepatocytes than rodents. Herein, we successfully developed three-dimensional (3D) culturing system for primary sheep and buffalo hepatocytes. The 3D-structures of sheep hepatocytes were formed on the fifth-d...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Meena Shri, Himanshu Agrawal, Payal Rani, Dheer Singh, Suneel Kumar Onteru
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3600cc958cb04be1bb9f79589f0d53b0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3600cc958cb04be1bb9f79589f0d53b0
record_format	dspace
spelling	oai:doaj.org-article:3600cc958cb04be1bb9f79589f0d53b02021-12-02T15:05:38ZHanging Drop, A Best Three-Dimensional (3D) Culture Method for Primary Buffalo and Sheep Hepatocytes10.1038/s41598-017-01355-62045-2322https://doaj.org/article/3600cc958cb04be1bb9f79589f0d53b02017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01355-6https://doaj.org/toc/2045-2322Abstract Livestock, having close resemblance to humans, could be a better source of primary hepatocytes than rodents. Herein, we successfully developed three-dimensional (3D) culturing system for primary sheep and buffalo hepatocytes. The 3D-structures of sheep hepatocytes were formed on the fifth-day and maintained until the tenth-day on polyHEMA-coated plates and in hanging drops with William’s E media (HDW). Between the cultured and fresh cells, we observed a similar expression of GAPDH, HNF4α, ALB, CYP1A1, CK8 and CK18. Interestingly, a statistically significant increase was noted in the TAT, CPS, AFP, AAT, GSP and PCNA expression. In buffalo hepatocytes culture, 3D-like structures were formed on the third-day and maintained until the sixth-day on polyHEMA and HDW. The expression of HNF4α, GSP, CPS, AFP, AAT, PCNA and CK18 was similar between cultured and fresh cells. Further, a statistically significant increase in the TAT and CK8 expression, and a decrease in the GAPDH, CYP1A1 and ALB expression were noted. Among the culture systems, HDW maintained the liver transcript markers more or less similar to the fresh hepatocytes of the sheep and buffalo for ten and six days, respectively. Taken together, hanging drop is an efficient method for 3D culturing of primary sheep and buffalo hepatocytes.Meena ShriHimanshu AgrawalPayal RaniDheer SinghSuneel Kumar OnteruNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Meena Shri Himanshu Agrawal Payal Rani Dheer Singh Suneel Kumar Onteru Hanging Drop, A Best Three-Dimensional (3D) Culture Method for Primary Buffalo and Sheep Hepatocytes
description	Abstract Livestock, having close resemblance to humans, could be a better source of primary hepatocytes than rodents. Herein, we successfully developed three-dimensional (3D) culturing system for primary sheep and buffalo hepatocytes. The 3D-structures of sheep hepatocytes were formed on the fifth-day and maintained until the tenth-day on polyHEMA-coated plates and in hanging drops with William’s E media (HDW). Between the cultured and fresh cells, we observed a similar expression of GAPDH, HNF4α, ALB, CYP1A1, CK8 and CK18. Interestingly, a statistically significant increase was noted in the TAT, CPS, AFP, AAT, GSP and PCNA expression. In buffalo hepatocytes culture, 3D-like structures were formed on the third-day and maintained until the sixth-day on polyHEMA and HDW. The expression of HNF4α, GSP, CPS, AFP, AAT, PCNA and CK18 was similar between cultured and fresh cells. Further, a statistically significant increase in the TAT and CK8 expression, and a decrease in the GAPDH, CYP1A1 and ALB expression were noted. Among the culture systems, HDW maintained the liver transcript markers more or less similar to the fresh hepatocytes of the sheep and buffalo for ten and six days, respectively. Taken together, hanging drop is an efficient method for 3D culturing of primary sheep and buffalo hepatocytes.
format	article
author	Meena Shri Himanshu Agrawal Payal Rani Dheer Singh Suneel Kumar Onteru
author_facet	Meena Shri Himanshu Agrawal Payal Rani Dheer Singh Suneel Kumar Onteru
author_sort	Meena Shri
title	Hanging Drop, A Best Three-Dimensional (3D) Culture Method for Primary Buffalo and Sheep Hepatocytes
title_short	Hanging Drop, A Best Three-Dimensional (3D) Culture Method for Primary Buffalo and Sheep Hepatocytes
title_full	Hanging Drop, A Best Three-Dimensional (3D) Culture Method for Primary Buffalo and Sheep Hepatocytes
title_fullStr	Hanging Drop, A Best Three-Dimensional (3D) Culture Method for Primary Buffalo and Sheep Hepatocytes
title_full_unstemmed	Hanging Drop, A Best Three-Dimensional (3D) Culture Method for Primary Buffalo and Sheep Hepatocytes
title_sort	hanging drop, a best three-dimensional (3d) culture method for primary buffalo and sheep hepatocytes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3600cc958cb04be1bb9f79589f0d53b0
work_keys_str_mv	AT meenashri hangingdropabestthreedimensional3dculturemethodforprimarybuffaloandsheephepatocytes AT himanshuagrawal hangingdropabestthreedimensional3dculturemethodforprimarybuffaloandsheephepatocytes AT payalrani hangingdropabestthreedimensional3dculturemethodforprimarybuffaloandsheephepatocytes AT dheersingh hangingdropabestthreedimensional3dculturemethodforprimarybuffaloandsheephepatocytes AT suneelkumaronteru hangingdropabestthreedimensional3dculturemethodforprimarybuffaloandsheephepatocytes
_version_	1718388757957181440

Hanging Drop, A Best Three-Dimensional (3D) Culture Method for Primary Buffalo and Sheep Hepatocytes

Ejemplares similares