The Performance of Seven Molecular Methods for the Detection of PRRSV

Porcine Reproductive and Respiratory Syndrome is a viral disease of swine characterized by reproductive failure of breeding animals and respiratory disorders in all categories. The first PRRS case in Serbia was recorded in 2001 after illegal import of boar semen. PRRS is economically the most import...

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Autores principales:	Milićević Vesna, Kureljušić Branislav, Veljović Ljubiša, Valčić Miroslav, Stević Nataša, Savić Božidar, Radojičić Sonja
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2020
Materias:	prrs rt-pcr sensitivity serbia Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/36050025e34b4eb188d82513002eea30
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spelling	oai:doaj.org-article:36050025e34b4eb188d82513002eea302021-11-17T21:27:53ZThe Performance of Seven Molecular Methods for the Detection of PRRSV1820-744810.2478/acve-2020-0003https://doaj.org/article/36050025e34b4eb188d82513002eea302020-03-01T00:00:00Zhttps://doi.org/10.2478/acve-2020-0003https://doaj.org/toc/1820-7448Porcine Reproductive and Respiratory Syndrome is a viral disease of swine characterized by reproductive failure of breeding animals and respiratory disorders in all categories. The first PRRS case in Serbia was recorded in 2001 after illegal import of boar semen. PRRS is economically the most important disease due to significant direct and indirect losses. Today, for routine diagnosis of PRRS in infected herds serological methods (ELISA) and molecular methods are used. Although modern diagnostic techniques are very robust, exceptional diversity of the viral strains is often the obstacle for an accurate diagnosis. To estimate the performance of seven different methods for PRRSV genome detection, twenty samples were used. However, none of the methods was able to detect all PRRSV strains. The best sensitivity was obtained by combining two methods. Until today, there is no absolutely accurate test which enables the detection of all circulating strains.Milićević VesnaKureljušić BranislavVeljović LjubišaValčić MiroslavStević NatašaSavić BožidarRadojičić SonjaSciendoarticleprrsrt-pcrsensitivityserbiaVeterinary medicineSF600-1100ENActa Veterinaria, Vol 70, Iss 1, Pp 51-57 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	prrs rt-pcr sensitivity serbia Veterinary medicine SF600-1100
spellingShingle	prrs rt-pcr sensitivity serbia Veterinary medicine SF600-1100 Milićević Vesna Kureljušić Branislav Veljović Ljubiša Valčić Miroslav Stević Nataša Savić Božidar Radojičić Sonja The Performance of Seven Molecular Methods for the Detection of PRRSV
description	Porcine Reproductive and Respiratory Syndrome is a viral disease of swine characterized by reproductive failure of breeding animals and respiratory disorders in all categories. The first PRRS case in Serbia was recorded in 2001 after illegal import of boar semen. PRRS is economically the most important disease due to significant direct and indirect losses. Today, for routine diagnosis of PRRS in infected herds serological methods (ELISA) and molecular methods are used. Although modern diagnostic techniques are very robust, exceptional diversity of the viral strains is often the obstacle for an accurate diagnosis. To estimate the performance of seven different methods for PRRSV genome detection, twenty samples were used. However, none of the methods was able to detect all PRRSV strains. The best sensitivity was obtained by combining two methods. Until today, there is no absolutely accurate test which enables the detection of all circulating strains.
format	article
author	Milićević Vesna Kureljušić Branislav Veljović Ljubiša Valčić Miroslav Stević Nataša Savić Božidar Radojičić Sonja
author_facet	Milićević Vesna Kureljušić Branislav Veljović Ljubiša Valčić Miroslav Stević Nataša Savić Božidar Radojičić Sonja
author_sort	Milićević Vesna
title	The Performance of Seven Molecular Methods for the Detection of PRRSV
title_short	The Performance of Seven Molecular Methods for the Detection of PRRSV
title_full	The Performance of Seven Molecular Methods for the Detection of PRRSV
title_fullStr	The Performance of Seven Molecular Methods for the Detection of PRRSV
title_full_unstemmed	The Performance of Seven Molecular Methods for the Detection of PRRSV
title_sort	performance of seven molecular methods for the detection of prrsv
publisher	Sciendo
publishDate	2020
url	https://doaj.org/article/36050025e34b4eb188d82513002eea30
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The Performance of Seven Molecular Methods for the Detection of PRRSV

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