Predominant infection of CD150+ lymphocytes and dendritic cells during measles virus infection of macaques.
Measles virus (MV) is hypothesized to enter the host by infecting epithelial cells of the respiratory tract, followed by viremia mediated by infected monocytes. However, neither of these cell types express signaling lymphocyte activation molecule (CD150), which has been identified as the receptor fo...
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2007
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oai:doaj.org-article:36055ec898274357aec538a1c469e3382021-12-02T19:59:50ZPredominant infection of CD150+ lymphocytes and dendritic cells during measles virus infection of macaques.1553-73661553-737410.1371/journal.ppat.0030178https://doaj.org/article/36055ec898274357aec538a1c469e3382007-11-01T00:00:00Zhttps://doi.org/10.1371/journal.ppat.0030178https://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Measles virus (MV) is hypothesized to enter the host by infecting epithelial cells of the respiratory tract, followed by viremia mediated by infected monocytes. However, neither of these cell types express signaling lymphocyte activation molecule (CD150), which has been identified as the receptor for wild-type MV. We have infected rhesus and cynomolgus macaques with a recombinant MV strain expressing enhanced green fluorescent protein (EGFP); thus bringing together the optimal animal model for measles and a virus that can be detected with unprecedented sensitivity. Blood samples and broncho-alveolar lavages were collected every 3 d, and necropsies were performed upon euthanasia 9 or 15 d after infection. EGFP production by MV-infected cells was visualized macroscopically, in both living and sacrificed animals, and microscopically by confocal microscopy and FACS analysis. At the peak of viremia, EGFP fluorescence was detected in skin, respiratory and digestive tract, but most intensely in all lymphoid tissues. B- and T-lymphocytes expressing CD150 were the major target cells for MV infection. Highest percentages (up to 30%) of infected lymphocytes were detected in lymphoid tissues, and the virus preferentially targeted cells with a memory phenotype. Unexpectedly, circulating monocytes did not sustain productive MV infection. In peripheral tissues, large numbers of MV-infected CD11c+ MHC class-II+ myeloid dendritic cells were detected in conjunction with infected T-lymphocytes, suggesting transmission of MV between these cell types. Fluorescent imaging of MV infection in non-human primates demonstrated a crucial role for lymphocytes and dendritic cells in the pathogenesis of measles and measles-associated immunosuppression.Rik L de SwartMartin LudlowLot de WitteYusuke YanagiGeert van AmerongenStephen McQuaidSelma YükselTeunis B H GeijtenbeekW Paul DuprexAlbert D M E OsterhausPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 3, Iss 11, p e178 (2007) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
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| topic |
Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 |
| spellingShingle |
Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Rik L de Swart Martin Ludlow Lot de Witte Yusuke Yanagi Geert van Amerongen Stephen McQuaid Selma Yüksel Teunis B H Geijtenbeek W Paul Duprex Albert D M E Osterhaus Predominant infection of CD150+ lymphocytes and dendritic cells during measles virus infection of macaques. |
| description |
Measles virus (MV) is hypothesized to enter the host by infecting epithelial cells of the respiratory tract, followed by viremia mediated by infected monocytes. However, neither of these cell types express signaling lymphocyte activation molecule (CD150), which has been identified as the receptor for wild-type MV. We have infected rhesus and cynomolgus macaques with a recombinant MV strain expressing enhanced green fluorescent protein (EGFP); thus bringing together the optimal animal model for measles and a virus that can be detected with unprecedented sensitivity. Blood samples and broncho-alveolar lavages were collected every 3 d, and necropsies were performed upon euthanasia 9 or 15 d after infection. EGFP production by MV-infected cells was visualized macroscopically, in both living and sacrificed animals, and microscopically by confocal microscopy and FACS analysis. At the peak of viremia, EGFP fluorescence was detected in skin, respiratory and digestive tract, but most intensely in all lymphoid tissues. B- and T-lymphocytes expressing CD150 were the major target cells for MV infection. Highest percentages (up to 30%) of infected lymphocytes were detected in lymphoid tissues, and the virus preferentially targeted cells with a memory phenotype. Unexpectedly, circulating monocytes did not sustain productive MV infection. In peripheral tissues, large numbers of MV-infected CD11c+ MHC class-II+ myeloid dendritic cells were detected in conjunction with infected T-lymphocytes, suggesting transmission of MV between these cell types. Fluorescent imaging of MV infection in non-human primates demonstrated a crucial role for lymphocytes and dendritic cells in the pathogenesis of measles and measles-associated immunosuppression. |
| format |
article |
| author |
Rik L de Swart Martin Ludlow Lot de Witte Yusuke Yanagi Geert van Amerongen Stephen McQuaid Selma Yüksel Teunis B H Geijtenbeek W Paul Duprex Albert D M E Osterhaus |
| author_facet |
Rik L de Swart Martin Ludlow Lot de Witte Yusuke Yanagi Geert van Amerongen Stephen McQuaid Selma Yüksel Teunis B H Geijtenbeek W Paul Duprex Albert D M E Osterhaus |
| author_sort |
Rik L de Swart |
| title |
Predominant infection of CD150+ lymphocytes and dendritic cells during measles virus infection of macaques. |
| title_short |
Predominant infection of CD150+ lymphocytes and dendritic cells during measles virus infection of macaques. |
| title_full |
Predominant infection of CD150+ lymphocytes and dendritic cells during measles virus infection of macaques. |
| title_fullStr |
Predominant infection of CD150+ lymphocytes and dendritic cells during measles virus infection of macaques. |
| title_full_unstemmed |
Predominant infection of CD150+ lymphocytes and dendritic cells during measles virus infection of macaques. |
| title_sort |
predominant infection of cd150+ lymphocytes and dendritic cells during measles virus infection of macaques. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2007 |
| url |
https://doaj.org/article/36055ec898274357aec538a1c469e338 |
| work_keys_str_mv |
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| _version_ |
1718375714034548736 |