Three month inhalation exposure to low-level PM2.5 induced brain toxicity in an Alzheimer's disease mouse model.

Although numerous epidemiological studies revealed an association between ambient fine particulate matter (PM2.5) exposure and Alzheimer's disease (AD), the PM2.5-induced neuron toxicity and associated mechanisms were not fully elucidated. The present study assessed brain toxicity in 6-month-ol...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sheng-Han Lee, Yi-Hsuan Chen, Chu-Chun Chien, Yuan-Horng Yan, Hsin-Chang Chen, Hsiao-Chi Chuang, Hui-I Hsieh, Kuan-Hung Cho, Li-Wei Kuo, Charles C-K Chou, Ming-Jang Chiu, Boon Lead Tee, Ta-Fu Chen, Tsun-Jen Cheng
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3606c9a7715c4a5699389bc19bce8dbc
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3606c9a7715c4a5699389bc19bce8dbc
record_format	dspace
spelling	oai:doaj.org-article:3606c9a7715c4a5699389bc19bce8dbc2021-12-02T20:17:34ZThree month inhalation exposure to low-level PM2.5 induced brain toxicity in an Alzheimer's disease mouse model.1932-620310.1371/journal.pone.0254587https://doaj.org/article/3606c9a7715c4a5699389bc19bce8dbc2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254587https://doaj.org/toc/1932-6203Although numerous epidemiological studies revealed an association between ambient fine particulate matter (PM2.5) exposure and Alzheimer's disease (AD), the PM2.5-induced neuron toxicity and associated mechanisms were not fully elucidated. The present study assessed brain toxicity in 6-month-old female triple-transgenic AD (3xTg-AD) mice following subchronic exposure to PM2.5 via an inhalation system. The treated mice were whole-bodily and continuously exposed to real-world PM2.5 for 3 months, while the control mice inhaled filtered air. Changes in cognitive and motor functions were evaluated using the Morris Water Maze and rotarod tests. Magnetic resonance imaging analysis was used to record gross brain volume alterations, and tissue staining with hematoxylin and eosin, Nissl, and immunohistochemistry methods were used to monitor pathological changes in microstructures after PM2.5 exposure. The levels of AD-related hallmarks and the oxidative stress biomarker malondialdehyde (MDA) were assessed using Western blot analysis and liquid chromatography-mass spectrometry, respectively. Our results showed that subchronic exposure to environmental levels of PM2.5 induced obvious neuronal loss in the cortex of exposed mice, but without significant impairment of cognitive and motor function. Increased levels of phosphorylated-tau and MDA were also observed in olfactory bulb or hippocampus after PM2.5 exposure, but no amyloid pathology was detected, as reported in previous studies. These results revealed that a relatively lower level of PM2.5 subchronic exposure from the environmental atmosphere still induced certain neurodegenerative changes in the brains of AD mice, especially in the olfactory bulb, entorhinal cortex and hippocampus, which is consistent with the nasal entry and spreading route for PM exposure. Systemic factors may also contribute to the neuronal toxicity. The effects of PM2.5 after a more prolonged exposure period are needed to establish a more comprehensive picture of the PM2.5-mediated development of AD.Sheng-Han LeeYi-Hsuan ChenChu-Chun ChienYuan-Horng YanHsin-Chang ChenHsiao-Chi ChuangHui-I HsiehKuan-Hung ChoLi-Wei KuoCharles C-K ChouMing-Jang ChiuBoon Lead TeeTa-Fu ChenTsun-Jen ChengPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0254587 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sheng-Han Lee Yi-Hsuan Chen Chu-Chun Chien Yuan-Horng Yan Hsin-Chang Chen Hsiao-Chi Chuang Hui-I Hsieh Kuan-Hung Cho Li-Wei Kuo Charles C-K Chou Ming-Jang Chiu Boon Lead Tee Ta-Fu Chen Tsun-Jen Cheng Three month inhalation exposure to low-level PM2.5 induced brain toxicity in an Alzheimer's disease mouse model.
description	Although numerous epidemiological studies revealed an association between ambient fine particulate matter (PM2.5) exposure and Alzheimer's disease (AD), the PM2.5-induced neuron toxicity and associated mechanisms were not fully elucidated. The present study assessed brain toxicity in 6-month-old female triple-transgenic AD (3xTg-AD) mice following subchronic exposure to PM2.5 via an inhalation system. The treated mice were whole-bodily and continuously exposed to real-world PM2.5 for 3 months, while the control mice inhaled filtered air. Changes in cognitive and motor functions were evaluated using the Morris Water Maze and rotarod tests. Magnetic resonance imaging analysis was used to record gross brain volume alterations, and tissue staining with hematoxylin and eosin, Nissl, and immunohistochemistry methods were used to monitor pathological changes in microstructures after PM2.5 exposure. The levels of AD-related hallmarks and the oxidative stress biomarker malondialdehyde (MDA) were assessed using Western blot analysis and liquid chromatography-mass spectrometry, respectively. Our results showed that subchronic exposure to environmental levels of PM2.5 induced obvious neuronal loss in the cortex of exposed mice, but without significant impairment of cognitive and motor function. Increased levels of phosphorylated-tau and MDA were also observed in olfactory bulb or hippocampus after PM2.5 exposure, but no amyloid pathology was detected, as reported in previous studies. These results revealed that a relatively lower level of PM2.5 subchronic exposure from the environmental atmosphere still induced certain neurodegenerative changes in the brains of AD mice, especially in the olfactory bulb, entorhinal cortex and hippocampus, which is consistent with the nasal entry and spreading route for PM exposure. Systemic factors may also contribute to the neuronal toxicity. The effects of PM2.5 after a more prolonged exposure period are needed to establish a more comprehensive picture of the PM2.5-mediated development of AD.
format	article
author	Sheng-Han Lee Yi-Hsuan Chen Chu-Chun Chien Yuan-Horng Yan Hsin-Chang Chen Hsiao-Chi Chuang Hui-I Hsieh Kuan-Hung Cho Li-Wei Kuo Charles C-K Chou Ming-Jang Chiu Boon Lead Tee Ta-Fu Chen Tsun-Jen Cheng
author_facet	Sheng-Han Lee Yi-Hsuan Chen Chu-Chun Chien Yuan-Horng Yan Hsin-Chang Chen Hsiao-Chi Chuang Hui-I Hsieh Kuan-Hung Cho Li-Wei Kuo Charles C-K Chou Ming-Jang Chiu Boon Lead Tee Ta-Fu Chen Tsun-Jen Cheng
author_sort	Sheng-Han Lee
title	Three month inhalation exposure to low-level PM2.5 induced brain toxicity in an Alzheimer's disease mouse model.
title_short	Three month inhalation exposure to low-level PM2.5 induced brain toxicity in an Alzheimer's disease mouse model.
title_full	Three month inhalation exposure to low-level PM2.5 induced brain toxicity in an Alzheimer's disease mouse model.
title_fullStr	Three month inhalation exposure to low-level PM2.5 induced brain toxicity in an Alzheimer's disease mouse model.
title_full_unstemmed	Three month inhalation exposure to low-level PM2.5 induced brain toxicity in an Alzheimer's disease mouse model.
title_sort	three month inhalation exposure to low-level pm2.5 induced brain toxicity in an alzheimer's disease mouse model.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/3606c9a7715c4a5699389bc19bce8dbc
work_keys_str_mv	AT shenghanlee threemonthinhalationexposuretolowlevelpm25inducedbraintoxicityinanalzheimersdiseasemousemodel AT yihsuanchen threemonthinhalationexposuretolowlevelpm25inducedbraintoxicityinanalzheimersdiseasemousemodel AT chuchunchien threemonthinhalationexposuretolowlevelpm25inducedbraintoxicityinanalzheimersdiseasemousemodel AT yuanhorngyan threemonthinhalationexposuretolowlevelpm25inducedbraintoxicityinanalzheimersdiseasemousemodel AT hsinchangchen threemonthinhalationexposuretolowlevelpm25inducedbraintoxicityinanalzheimersdiseasemousemodel AT hsiaochichuang threemonthinhalationexposuretolowlevelpm25inducedbraintoxicityinanalzheimersdiseasemousemodel AT huiihsieh threemonthinhalationexposuretolowlevelpm25inducedbraintoxicityinanalzheimersdiseasemousemodel AT kuanhungcho threemonthinhalationexposuretolowlevelpm25inducedbraintoxicityinanalzheimersdiseasemousemodel AT liweikuo threemonthinhalationexposuretolowlevelpm25inducedbraintoxicityinanalzheimersdiseasemousemodel AT charlesckchou threemonthinhalationexposuretolowlevelpm25inducedbraintoxicityinanalzheimersdiseasemousemodel AT mingjangchiu threemonthinhalationexposuretolowlevelpm25inducedbraintoxicityinanalzheimersdiseasemousemodel AT boonleadtee threemonthinhalationexposuretolowlevelpm25inducedbraintoxicityinanalzheimersdiseasemousemodel AT tafuchen threemonthinhalationexposuretolowlevelpm25inducedbraintoxicityinanalzheimersdiseasemousemodel AT tsunjencheng threemonthinhalationexposuretolowlevelpm25inducedbraintoxicityinanalzheimersdiseasemousemodel
_version_	1718374338011332608

Three month inhalation exposure to low-level PM2.5 induced brain toxicity in an Alzheimer's disease mouse model.

Ejemplares similares