Intraocular Viral Communities Associated With Post-fever Retinitis

The virome of ocular fluids is naive. The results of this study highlight the virome in the vitreous fluid of the eye of individuals without any ocular infection and compare it with the virome of the vitreous fluid of individuals with retinitis. A total of 1,016,037 viral reads were generated from 2...

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Autores principales:	Kotakonda Arunasri, Gumpili Sai Prashanthi, Mudit Tyagi, Rajeev R. Pappuru, Sisinthy Shivaji
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	metagenomic sequencing vitreous fluid post-fever retinitis changes in virome ocular pathogen human viruses Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3607cefea2d3480aa1a739fef382a3fb
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spelling	oai:doaj.org-article:3607cefea2d3480aa1a739fef382a3fb2021-11-19T05:10:19ZIntraocular Viral Communities Associated With Post-fever Retinitis2296-858X10.3389/fmed.2021.724195https://doaj.org/article/3607cefea2d3480aa1a739fef382a3fb2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmed.2021.724195/fullhttps://doaj.org/toc/2296-858XThe virome of ocular fluids is naive. The results of this study highlight the virome in the vitreous fluid of the eye of individuals without any ocular infection and compare it with the virome of the vitreous fluid of individuals with retinitis. A total of 1,016,037 viral reads were generated from 25 vitreous fluid samples comprising control and post-fever retinitis (PFR) samples. The top 10 viral families in the vitreous fluids comprised of Myoviridae, Siphoviridae, Phycodnaviridae, Herpesviridae, Poxviridae, Iridoviridae, Podoviridae, Retroviridae, Baculoviridae, and Flaviviridae. Principal coordinate analysis and heat map analysis clearly discriminated the virome of the vitreous fluid of the controls from that of the PFR virome. The abundance of 10 viral genera increased significantly in the vitreous fluid virome of the post-fever retinitis group compared with the control group. Genus Lymphocryptovirus, comprising the human pathogen Epstein-Barr virus (EBV) that is also implicated in ocular infections was significantly abundant in eight out of the nine vitreous fluid viromes of post-fever retinitis group samples compared with the control viromes. Human viruses, such as Hepacivirus, Circovirus, and Kobuvirus, were also significantly increased in abundance in the vitreous fluid viromes of post-fever retinitis group samples compared with the control viromes. The Kyoto Encyclopedia of Genes and Genomes (KEGG) functional analysis and the network analysis depicted an increase in the immune response by the host in the post-fever retinitis group compared with the control group. All together, the results of the study indicate changes in the virome in the vitreous fluid of patients with the post-fever retinitis group compared to the control group.Kotakonda ArunasriGumpili Sai PrashanthiMudit TyagiRajeev R. PappuruSisinthy ShivajiFrontiers Media S.A.articlemetagenomic sequencingvitreous fluidpost-fever retinitischanges in viromeocular pathogenhuman virusesMedicine (General)R5-920ENFrontiers in Medicine, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	metagenomic sequencing vitreous fluid post-fever retinitis changes in virome ocular pathogen human viruses Medicine (General) R5-920
spellingShingle	metagenomic sequencing vitreous fluid post-fever retinitis changes in virome ocular pathogen human viruses Medicine (General) R5-920 Kotakonda Arunasri Gumpili Sai Prashanthi Mudit Tyagi Rajeev R. Pappuru Sisinthy Shivaji Intraocular Viral Communities Associated With Post-fever Retinitis
description	The virome of ocular fluids is naive. The results of this study highlight the virome in the vitreous fluid of the eye of individuals without any ocular infection and compare it with the virome of the vitreous fluid of individuals with retinitis. A total of 1,016,037 viral reads were generated from 25 vitreous fluid samples comprising control and post-fever retinitis (PFR) samples. The top 10 viral families in the vitreous fluids comprised of Myoviridae, Siphoviridae, Phycodnaviridae, Herpesviridae, Poxviridae, Iridoviridae, Podoviridae, Retroviridae, Baculoviridae, and Flaviviridae. Principal coordinate analysis and heat map analysis clearly discriminated the virome of the vitreous fluid of the controls from that of the PFR virome. The abundance of 10 viral genera increased significantly in the vitreous fluid virome of the post-fever retinitis group compared with the control group. Genus Lymphocryptovirus, comprising the human pathogen Epstein-Barr virus (EBV) that is also implicated in ocular infections was significantly abundant in eight out of the nine vitreous fluid viromes of post-fever retinitis group samples compared with the control viromes. Human viruses, such as Hepacivirus, Circovirus, and Kobuvirus, were also significantly increased in abundance in the vitreous fluid viromes of post-fever retinitis group samples compared with the control viromes. The Kyoto Encyclopedia of Genes and Genomes (KEGG) functional analysis and the network analysis depicted an increase in the immune response by the host in the post-fever retinitis group compared with the control group. All together, the results of the study indicate changes in the virome in the vitreous fluid of patients with the post-fever retinitis group compared to the control group.
format	article
author	Kotakonda Arunasri Gumpili Sai Prashanthi Mudit Tyagi Rajeev R. Pappuru Sisinthy Shivaji
author_facet	Kotakonda Arunasri Gumpili Sai Prashanthi Mudit Tyagi Rajeev R. Pappuru Sisinthy Shivaji
author_sort	Kotakonda Arunasri
title	Intraocular Viral Communities Associated With Post-fever Retinitis
title_short	Intraocular Viral Communities Associated With Post-fever Retinitis
title_full	Intraocular Viral Communities Associated With Post-fever Retinitis
title_fullStr	Intraocular Viral Communities Associated With Post-fever Retinitis
title_full_unstemmed	Intraocular Viral Communities Associated With Post-fever Retinitis
title_sort	intraocular viral communities associated with post-fever retinitis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/3607cefea2d3480aa1a739fef382a3fb
work_keys_str_mv	AT kotakondaarunasri intraocularviralcommunitiesassociatedwithpostfeverretinitis AT gumpilisaiprashanthi intraocularviralcommunitiesassociatedwithpostfeverretinitis AT mudittyagi intraocularviralcommunitiesassociatedwithpostfeverretinitis AT rajeevrpappuru intraocularviralcommunitiesassociatedwithpostfeverretinitis AT sisinthyshivaji intraocularviralcommunitiesassociatedwithpostfeverretinitis
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Intraocular Viral Communities Associated With Post-fever Retinitis

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