Optical coherence tomography and multiphoton microscopy offer new options for the quantification of fibrotic aortic valve disease in ApoE−/− mice

Abstract Aortic valve sclerosis is characterized as the thickening of the aortic valve without obstruction of the left ventricular outflow. It has a prevalence of 30% in people over 65 years old. Aortic valve sclerosis represents a cardiovascular risk marker because it may progress to moderate or se...

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Autores principales:	Anett Jannasch, Christian Schnabel, Roberta Galli, Saskia Faak, Petra Büttner, Claudia Dittfeld, Sems Malte Tugtekin, Edmund Koch, Klaus Matschke
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/36083ad58ce84f6fba09f3c003e4e4fd
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spelling	oai:doaj.org-article:36083ad58ce84f6fba09f3c003e4e4fd2021-12-02T15:52:29ZOptical coherence tomography and multiphoton microscopy offer new options for the quantification of fibrotic aortic valve disease in ApoE−/− mice10.1038/s41598-021-85142-42045-2322https://doaj.org/article/36083ad58ce84f6fba09f3c003e4e4fd2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85142-4https://doaj.org/toc/2045-2322Abstract Aortic valve sclerosis is characterized as the thickening of the aortic valve without obstruction of the left ventricular outflow. It has a prevalence of 30% in people over 65 years old. Aortic valve sclerosis represents a cardiovascular risk marker because it may progress to moderate or severe aortic valve stenosis. Thus, the early recognition and management of aortic valve sclerosis are of cardinal importance. We examined the aortic valve geometry and structure from healthy C57Bl6 wild type and age-matched hyperlipidemic ApoE−/− mice with aortic valve sclerosis using optical coherence tomography (OCT) and multiphoton microscopy (MPM) and compared results with histological analyses. Early fibrotic thickening, especially in the tip region of the native aortic valve leaflets from the ApoE−/− mice, was detectable in a precise spatial resolution using OCT. Evaluation of the second harmonic generation signal using MPM demonstrated that collagen content decreased in all aortic valve leaflet regions in the ApoE−/− mice. Lipid droplets and cholesterol crystals were detected using coherent anti-Stokes Raman scattering in the tissue from the ApoE−/− mice. Here, we demonstrated that OCT and MPM, which are fast and precise contactless imaging approaches, are suitable for defining early morphological and structural alterations of sclerotic murine aortic valves.Anett JannaschChristian SchnabelRoberta GalliSaskia FaakPetra BüttnerClaudia DittfeldSems Malte TugtekinEdmund KochKlaus MatschkeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anett Jannasch Christian Schnabel Roberta Galli Saskia Faak Petra Büttner Claudia Dittfeld Sems Malte Tugtekin Edmund Koch Klaus Matschke Optical coherence tomography and multiphoton microscopy offer new options for the quantification of fibrotic aortic valve disease in ApoE−/− mice
description	Abstract Aortic valve sclerosis is characterized as the thickening of the aortic valve without obstruction of the left ventricular outflow. It has a prevalence of 30% in people over 65 years old. Aortic valve sclerosis represents a cardiovascular risk marker because it may progress to moderate or severe aortic valve stenosis. Thus, the early recognition and management of aortic valve sclerosis are of cardinal importance. We examined the aortic valve geometry and structure from healthy C57Bl6 wild type and age-matched hyperlipidemic ApoE−/− mice with aortic valve sclerosis using optical coherence tomography (OCT) and multiphoton microscopy (MPM) and compared results with histological analyses. Early fibrotic thickening, especially in the tip region of the native aortic valve leaflets from the ApoE−/− mice, was detectable in a precise spatial resolution using OCT. Evaluation of the second harmonic generation signal using MPM demonstrated that collagen content decreased in all aortic valve leaflet regions in the ApoE−/− mice. Lipid droplets and cholesterol crystals were detected using coherent anti-Stokes Raman scattering in the tissue from the ApoE−/− mice. Here, we demonstrated that OCT and MPM, which are fast and precise contactless imaging approaches, are suitable for defining early morphological and structural alterations of sclerotic murine aortic valves.
format	article
author	Anett Jannasch Christian Schnabel Roberta Galli Saskia Faak Petra Büttner Claudia Dittfeld Sems Malte Tugtekin Edmund Koch Klaus Matschke
author_facet	Anett Jannasch Christian Schnabel Roberta Galli Saskia Faak Petra Büttner Claudia Dittfeld Sems Malte Tugtekin Edmund Koch Klaus Matschke
author_sort	Anett Jannasch
title	Optical coherence tomography and multiphoton microscopy offer new options for the quantification of fibrotic aortic valve disease in ApoE−/− mice
title_short	Optical coherence tomography and multiphoton microscopy offer new options for the quantification of fibrotic aortic valve disease in ApoE−/− mice
title_full	Optical coherence tomography and multiphoton microscopy offer new options for the quantification of fibrotic aortic valve disease in ApoE−/− mice
title_fullStr	Optical coherence tomography and multiphoton microscopy offer new options for the quantification of fibrotic aortic valve disease in ApoE−/− mice
title_full_unstemmed	Optical coherence tomography and multiphoton microscopy offer new options for the quantification of fibrotic aortic valve disease in ApoE−/− mice
title_sort	optical coherence tomography and multiphoton microscopy offer new options for the quantification of fibrotic aortic valve disease in apoe−/− mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/36083ad58ce84f6fba09f3c003e4e4fd
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Optical coherence tomography and multiphoton microscopy offer new options for the quantification of fibrotic aortic valve disease in ApoE−/− mice

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