Utilization of silicon nanowire field-effect transistors for the detection of a cardiac biomarker, cardiac troponin I and their applications involving animal models
Abstract This study develops an ultrasensitive electrical device, the silicon nanowire-field effect transistor (SiNW-FET) for detection of cardiac troponin I (cTnI) in obesity induced myocardial injury. The biosensor device utilizes metal–oxide–semiconductor (MOS) compatible top-down methodology for...
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Nature Portfolio
2020
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oai:doaj.org-article:3609e10ae46d4a9fbbbd58aa3054cceb2021-12-02T13:58:25ZUtilization of silicon nanowire field-effect transistors for the detection of a cardiac biomarker, cardiac troponin I and their applications involving animal models10.1038/s41598-020-78829-72045-2322https://doaj.org/article/3609e10ae46d4a9fbbbd58aa3054cceb2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78829-7https://doaj.org/toc/2045-2322Abstract This study develops an ultrasensitive electrical device, the silicon nanowire-field effect transistor (SiNW-FET) for detection of cardiac troponin I (cTnI) in obesity induced myocardial injury. The biosensor device utilizes metal–oxide–semiconductor (MOS) compatible top-down methodology for the fabrication process. After fabrication, the surface of the SiNW is modified with the cTnI monoclonal antibody (Mab-cTnI) upon covalent immobilization to capture cTnI antigen. The sensitivity of the device is also examined using cTnI at different concentrations with the lowest detection limit of 0.016 ng/mL. The electrocardiogram (ECG), magnetic resonance imaging (MRI), and superior vena cave (SVC) provide more information about cardiac responses in a mouse model of acute myocardial infarction (AMI). Further, magnetic resonance imaging helps to evaluate the cardiac output of an obesity induced myocardial injury mouse model. These methods play an essential role in monitoring the obesity based cardiac injury and hence, these studies were carried out. This is the first report to use the ECG, MRI, and SVC sampling methods to study the obesity based cardiac injury involving Syrian hamsters as animal models. The proposed SiNW-FET in this study shows greater sensitivity than the previously developed devices and demonstrates great potential for future applications in point-of-care (POC) diagnosis.Shih-Mein ChangSathyadevi PalanisamyTung-Ho WuChiao-Yun ChenKai-Hung ChengChen-Yi LeeShyng-Shiou F. YuanYun-Ming WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020) |
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Medicine R Science Q Shih-Mein Chang Sathyadevi Palanisamy Tung-Ho Wu Chiao-Yun Chen Kai-Hung Cheng Chen-Yi Lee Shyng-Shiou F. Yuan Yun-Ming Wang Utilization of silicon nanowire field-effect transistors for the detection of a cardiac biomarker, cardiac troponin I and their applications involving animal models |
| description |
Abstract This study develops an ultrasensitive electrical device, the silicon nanowire-field effect transistor (SiNW-FET) for detection of cardiac troponin I (cTnI) in obesity induced myocardial injury. The biosensor device utilizes metal–oxide–semiconductor (MOS) compatible top-down methodology for the fabrication process. After fabrication, the surface of the SiNW is modified with the cTnI monoclonal antibody (Mab-cTnI) upon covalent immobilization to capture cTnI antigen. The sensitivity of the device is also examined using cTnI at different concentrations with the lowest detection limit of 0.016 ng/mL. The electrocardiogram (ECG), magnetic resonance imaging (MRI), and superior vena cave (SVC) provide more information about cardiac responses in a mouse model of acute myocardial infarction (AMI). Further, magnetic resonance imaging helps to evaluate the cardiac output of an obesity induced myocardial injury mouse model. These methods play an essential role in monitoring the obesity based cardiac injury and hence, these studies were carried out. This is the first report to use the ECG, MRI, and SVC sampling methods to study the obesity based cardiac injury involving Syrian hamsters as animal models. The proposed SiNW-FET in this study shows greater sensitivity than the previously developed devices and demonstrates great potential for future applications in point-of-care (POC) diagnosis. |
| format |
article |
| author |
Shih-Mein Chang Sathyadevi Palanisamy Tung-Ho Wu Chiao-Yun Chen Kai-Hung Cheng Chen-Yi Lee Shyng-Shiou F. Yuan Yun-Ming Wang |
| author_facet |
Shih-Mein Chang Sathyadevi Palanisamy Tung-Ho Wu Chiao-Yun Chen Kai-Hung Cheng Chen-Yi Lee Shyng-Shiou F. Yuan Yun-Ming Wang |
| author_sort |
Shih-Mein Chang |
| title |
Utilization of silicon nanowire field-effect transistors for the detection of a cardiac biomarker, cardiac troponin I and their applications involving animal models |
| title_short |
Utilization of silicon nanowire field-effect transistors for the detection of a cardiac biomarker, cardiac troponin I and their applications involving animal models |
| title_full |
Utilization of silicon nanowire field-effect transistors for the detection of a cardiac biomarker, cardiac troponin I and their applications involving animal models |
| title_fullStr |
Utilization of silicon nanowire field-effect transistors for the detection of a cardiac biomarker, cardiac troponin I and their applications involving animal models |
| title_full_unstemmed |
Utilization of silicon nanowire field-effect transistors for the detection of a cardiac biomarker, cardiac troponin I and their applications involving animal models |
| title_sort |
utilization of silicon nanowire field-effect transistors for the detection of a cardiac biomarker, cardiac troponin i and their applications involving animal models |
| publisher |
Nature Portfolio |
| publishDate |
2020 |
| url |
https://doaj.org/article/3609e10ae46d4a9fbbbd58aa3054cceb |
| work_keys_str_mv |
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