Saponin 1 induces apoptosis and suppresses NF-κB-mediated survival signaling in glioblastoma multiforme (GBM).

Saponin 1 is a triterpeniod saponin extracted from Anemone taipaiensis, a traditional Chinese medicine against rheumatism and phlebitis. It has also been shown to exhibit significant anti-tumor activity against human leukemia (HL-60 cells) and human hepatocellular carcinoma (Hep-G2 cells). Herein we...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Juan Li, Haifeng Tang, Yun Zhang, Chi Tang, Bo Li, Yuangang Wang, Zhenhui Gao, Peng Luo, Anan Yin, Xiaoyang Wang, Guang Cheng, Zhou Fei
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/360bfc0babcd48bf8c9603898a4968b7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:360bfc0babcd48bf8c9603898a4968b7
record_format	dspace
spelling	oai:doaj.org-article:360bfc0babcd48bf8c9603898a4968b72021-11-18T08:45:09ZSaponin 1 induces apoptosis and suppresses NF-κB-mediated survival signaling in glioblastoma multiforme (GBM).1932-620310.1371/journal.pone.0081258https://doaj.org/article/360bfc0babcd48bf8c9603898a4968b72013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24278406/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Saponin 1 is a triterpeniod saponin extracted from Anemone taipaiensis, a traditional Chinese medicine against rheumatism and phlebitis. It has also been shown to exhibit significant anti-tumor activity against human leukemia (HL-60 cells) and human hepatocellular carcinoma (Hep-G2 cells). Herein we investigated the effect of saponin 1 in human glioblastoma multiforme (GBM) U251MG and U87MG cells. Saponin 1 induced significant growth inhibition in both glioblastoma cell lines, with a 50% inhibitory concentration at 24 h of 7.4 µg/ml in U251MG cells and 8.6 µg/ml in U87MG cells, respectively. Nuclear fluorescent staining and electron microscopy showed that saponin 1 caused characteristic apoptotic morphological changes in the GBM cell lines. Saponin 1-induced apoptosis was also verified by DNA ladder electrophoresis and flow cytometry. Additionally, immunocytochemistry and western blotting analyses revealed a time-dependent decrease in the expression and nuclear location of NF-κB following saponin 1 treatment. Western blotting data indicated a significant decreased expression of inhibitors of apoptosis (IAP) family members,(e.g., survivin and XIAP) by saponin 1. Moreover, saponin 1 caused a decrease in the Bcl-2/Bax ratio and initiated apoptosis by activating caspase-9 and caspase-3 in the GBM cell lines. These findings indicate that saponin 1 inhibits cell growth of GBM cells at least partially by inducing apoptosis and inhibiting survival signaling mediated by NF-κB. In addition, in vivo study also demonstrated an obvious inhibition of saponin 1 treatment on the tumor growth of U251MG and U87MG cells-produced xenograft tumors in nude mice. Given the minimal toxicities of saponin 1 in non-neoplastic astrocytes, our results suggest that saponin 1 exhibits significant in vitro and in vivo anti-tumor efficacy and merits further investigation as a potential therapeutic agent for GBM.Juan LiHaifeng TangYun ZhangChi TangBo LiYuangang WangZhenhui GaoPeng LuoAnan YinXiaoyang WangGuang ChengZhou FeiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e81258 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Juan Li Haifeng Tang Yun Zhang Chi Tang Bo Li Yuangang Wang Zhenhui Gao Peng Luo Anan Yin Xiaoyang Wang Guang Cheng Zhou Fei Saponin 1 induces apoptosis and suppresses NF-κB-mediated survival signaling in glioblastoma multiforme (GBM).
description	Saponin 1 is a triterpeniod saponin extracted from Anemone taipaiensis, a traditional Chinese medicine against rheumatism and phlebitis. It has also been shown to exhibit significant anti-tumor activity against human leukemia (HL-60 cells) and human hepatocellular carcinoma (Hep-G2 cells). Herein we investigated the effect of saponin 1 in human glioblastoma multiforme (GBM) U251MG and U87MG cells. Saponin 1 induced significant growth inhibition in both glioblastoma cell lines, with a 50% inhibitory concentration at 24 h of 7.4 µg/ml in U251MG cells and 8.6 µg/ml in U87MG cells, respectively. Nuclear fluorescent staining and electron microscopy showed that saponin 1 caused characteristic apoptotic morphological changes in the GBM cell lines. Saponin 1-induced apoptosis was also verified by DNA ladder electrophoresis and flow cytometry. Additionally, immunocytochemistry and western blotting analyses revealed a time-dependent decrease in the expression and nuclear location of NF-κB following saponin 1 treatment. Western blotting data indicated a significant decreased expression of inhibitors of apoptosis (IAP) family members,(e.g., survivin and XIAP) by saponin 1. Moreover, saponin 1 caused a decrease in the Bcl-2/Bax ratio and initiated apoptosis by activating caspase-9 and caspase-3 in the GBM cell lines. These findings indicate that saponin 1 inhibits cell growth of GBM cells at least partially by inducing apoptosis and inhibiting survival signaling mediated by NF-κB. In addition, in vivo study also demonstrated an obvious inhibition of saponin 1 treatment on the tumor growth of U251MG and U87MG cells-produced xenograft tumors in nude mice. Given the minimal toxicities of saponin 1 in non-neoplastic astrocytes, our results suggest that saponin 1 exhibits significant in vitro and in vivo anti-tumor efficacy and merits further investigation as a potential therapeutic agent for GBM.
format	article
author	Juan Li Haifeng Tang Yun Zhang Chi Tang Bo Li Yuangang Wang Zhenhui Gao Peng Luo Anan Yin Xiaoyang Wang Guang Cheng Zhou Fei
author_facet	Juan Li Haifeng Tang Yun Zhang Chi Tang Bo Li Yuangang Wang Zhenhui Gao Peng Luo Anan Yin Xiaoyang Wang Guang Cheng Zhou Fei
author_sort	Juan Li
title	Saponin 1 induces apoptosis and suppresses NF-κB-mediated survival signaling in glioblastoma multiforme (GBM).
title_short	Saponin 1 induces apoptosis and suppresses NF-κB-mediated survival signaling in glioblastoma multiforme (GBM).
title_full	Saponin 1 induces apoptosis and suppresses NF-κB-mediated survival signaling in glioblastoma multiforme (GBM).
title_fullStr	Saponin 1 induces apoptosis and suppresses NF-κB-mediated survival signaling in glioblastoma multiforme (GBM).
title_full_unstemmed	Saponin 1 induces apoptosis and suppresses NF-κB-mediated survival signaling in glioblastoma multiforme (GBM).
title_sort	saponin 1 induces apoptosis and suppresses nf-κb-mediated survival signaling in glioblastoma multiforme (gbm).
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/360bfc0babcd48bf8c9603898a4968b7
work_keys_str_mv	AT juanli saponin1inducesapoptosisandsuppressesnfkbmediatedsurvivalsignalinginglioblastomamultiformegbm AT haifengtang saponin1inducesapoptosisandsuppressesnfkbmediatedsurvivalsignalinginglioblastomamultiformegbm AT yunzhang saponin1inducesapoptosisandsuppressesnfkbmediatedsurvivalsignalinginglioblastomamultiformegbm AT chitang saponin1inducesapoptosisandsuppressesnfkbmediatedsurvivalsignalinginglioblastomamultiformegbm AT boli saponin1inducesapoptosisandsuppressesnfkbmediatedsurvivalsignalinginglioblastomamultiformegbm AT yuangangwang saponin1inducesapoptosisandsuppressesnfkbmediatedsurvivalsignalinginglioblastomamultiformegbm AT zhenhuigao saponin1inducesapoptosisandsuppressesnfkbmediatedsurvivalsignalinginglioblastomamultiformegbm AT pengluo saponin1inducesapoptosisandsuppressesnfkbmediatedsurvivalsignalinginglioblastomamultiformegbm AT ananyin saponin1inducesapoptosisandsuppressesnfkbmediatedsurvivalsignalinginglioblastomamultiformegbm AT xiaoyangwang saponin1inducesapoptosisandsuppressesnfkbmediatedsurvivalsignalinginglioblastomamultiformegbm AT guangcheng saponin1inducesapoptosisandsuppressesnfkbmediatedsurvivalsignalinginglioblastomamultiformegbm AT zhoufei saponin1inducesapoptosisandsuppressesnfkbmediatedsurvivalsignalinginglioblastomamultiformegbm
_version_	1718421375335530496

Saponin 1 induces apoptosis and suppresses NF-κB-mediated survival signaling in glioblastoma multiforme (GBM).

Ejemplares similares