Generation of human melanocytes from induced pluripotent stem cells.

Epidermal melanocytes play an important role in protecting the skin from UV rays, and their functional impairment results in pigment disorders. Additionally, melanomas are considered to arise from mutations that accumulate in melanocyte stem cells. The mechanisms underlying melanocyte differentiatio...

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Autores principales:	Shigeki Ohta, Yoichi Imaizumi, Yohei Okada, Wado Akamatsu, Reiko Kuwahara, Manabu Ohyama, Masayuki Amagai, Yumi Matsuzaki, Shinya Yamanaka, Hideyuki Okano, Yutaka Kawakami
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/360cbd18bfe44ad7a4b777094a9f94c6
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spelling	oai:doaj.org-article:360cbd18bfe44ad7a4b777094a9f94c62021-11-18T07:00:30ZGeneration of human melanocytes from induced pluripotent stem cells.1932-620310.1371/journal.pone.0016182https://doaj.org/article/360cbd18bfe44ad7a4b777094a9f94c62011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21249204/?tool=EBIhttps://doaj.org/toc/1932-6203Epidermal melanocytes play an important role in protecting the skin from UV rays, and their functional impairment results in pigment disorders. Additionally, melanomas are considered to arise from mutations that accumulate in melanocyte stem cells. The mechanisms underlying melanocyte differentiation and the defining characteristics of melanocyte stem cells in humans are, however, largely unknown. In the present study, we set out to generate melanocytes from human iPS cells in vitro, leading to a preliminary investigation of the mechanisms of human melanocyte differentiation. We generated iPS cell lines from human dermal fibroblasts using the Yamanaka factors (SOX2, OCT3/4, and KLF4, with or without c-MYC). These iPS cell lines were subsequently used to form embryoid bodies (EBs) and then differentiated into melanocytes via culture supplementation with Wnt3a, SCF, and ET-3. Seven weeks after inducing differentiation, pigmented cells expressing melanocyte markers such as MITF, tyrosinase, SILV, and TYRP1, were detected. Melanosomes were identified in these pigmented cells by electron microscopy, and global gene expression profiling of the pigmented cells showed a high similarity to that of human primary foreskin-derived melanocytes, suggesting the successful generation of melanocytes from iPS cells. This in vitro differentiation system should prove useful for understanding human melanocyte biology and revealing the mechanism of various pigment cell disorders, including melanoma.Shigeki OhtaYoichi ImaizumiYohei OkadaWado AkamatsuReiko KuwaharaManabu OhyamaMasayuki AmagaiYumi MatsuzakiShinya YamanakaHideyuki OkanoYutaka KawakamiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 1, p e16182 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shigeki Ohta Yoichi Imaizumi Yohei Okada Wado Akamatsu Reiko Kuwahara Manabu Ohyama Masayuki Amagai Yumi Matsuzaki Shinya Yamanaka Hideyuki Okano Yutaka Kawakami Generation of human melanocytes from induced pluripotent stem cells.
description	Epidermal melanocytes play an important role in protecting the skin from UV rays, and their functional impairment results in pigment disorders. Additionally, melanomas are considered to arise from mutations that accumulate in melanocyte stem cells. The mechanisms underlying melanocyte differentiation and the defining characteristics of melanocyte stem cells in humans are, however, largely unknown. In the present study, we set out to generate melanocytes from human iPS cells in vitro, leading to a preliminary investigation of the mechanisms of human melanocyte differentiation. We generated iPS cell lines from human dermal fibroblasts using the Yamanaka factors (SOX2, OCT3/4, and KLF4, with or without c-MYC). These iPS cell lines were subsequently used to form embryoid bodies (EBs) and then differentiated into melanocytes via culture supplementation with Wnt3a, SCF, and ET-3. Seven weeks after inducing differentiation, pigmented cells expressing melanocyte markers such as MITF, tyrosinase, SILV, and TYRP1, were detected. Melanosomes were identified in these pigmented cells by electron microscopy, and global gene expression profiling of the pigmented cells showed a high similarity to that of human primary foreskin-derived melanocytes, suggesting the successful generation of melanocytes from iPS cells. This in vitro differentiation system should prove useful for understanding human melanocyte biology and revealing the mechanism of various pigment cell disorders, including melanoma.
format	article
author	Shigeki Ohta Yoichi Imaizumi Yohei Okada Wado Akamatsu Reiko Kuwahara Manabu Ohyama Masayuki Amagai Yumi Matsuzaki Shinya Yamanaka Hideyuki Okano Yutaka Kawakami
author_facet	Shigeki Ohta Yoichi Imaizumi Yohei Okada Wado Akamatsu Reiko Kuwahara Manabu Ohyama Masayuki Amagai Yumi Matsuzaki Shinya Yamanaka Hideyuki Okano Yutaka Kawakami
author_sort	Shigeki Ohta
title	Generation of human melanocytes from induced pluripotent stem cells.
title_short	Generation of human melanocytes from induced pluripotent stem cells.
title_full	Generation of human melanocytes from induced pluripotent stem cells.
title_fullStr	Generation of human melanocytes from induced pluripotent stem cells.
title_full_unstemmed	Generation of human melanocytes from induced pluripotent stem cells.
title_sort	generation of human melanocytes from induced pluripotent stem cells.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/360cbd18bfe44ad7a4b777094a9f94c6
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Generation of human melanocytes from induced pluripotent stem cells.

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