Zona pellucida domain-containing protein β-tectorin is crucial for zebrafish proper inner ear development.

<h4>Background</h4>The zona pellucida (ZP) domain is part of many extracellular proteins with diverse functions from structural components to receptors. The mammalian β-tectorin is a protein of 336 amino acid residues containing a single ZP domain and a putative signal peptide at the N-t...

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Autores principales:	Chung-Hsiang Yang, Chia-Hsiung Cheng, Gen-Der Chen, Wei-Hao Liao, Yi-Chung Chen, Kai-Yun Huang, Pung-Pung Hwang, Sheng-Ping L Hwang, Chang-Jen Huang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/360ecf0ff1f447c1b398548de2adbfb8
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spelling	oai:doaj.org-article:360ecf0ff1f447c1b398548de2adbfb82021-11-18T06:48:50ZZona pellucida domain-containing protein β-tectorin is crucial for zebrafish proper inner ear development.1932-620310.1371/journal.pone.0023078https://doaj.org/article/360ecf0ff1f447c1b398548de2adbfb82011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21829695/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The zona pellucida (ZP) domain is part of many extracellular proteins with diverse functions from structural components to receptors. The mammalian β-tectorin is a protein of 336 amino acid residues containing a single ZP domain and a putative signal peptide at the N-terminus of the protein. It is 1 component of a gel-like structure called the tectorial membrane which is involved in transforming sound waves into neuronal signals and is important for normal auditory function. β-Tectorin is specifically expressed in the mammalian and avian inner ear.<h4>Methodology/principal findings</h4>We identified and cloned the gene encoding zebrafish β-tectorin. Through whole-mount in situ hybridization, we demonstrated that β-tectorin messenger RNA was expressed in the otic placode and specialized sensory patch of the inner ear during zebrafish embryonic stages. Morpholino knockdown of zebrafish β-tectorin affected the position and number of otoliths in the ears of morphants. Finally, swimming behaviors of β-tectorin morphants were abnormal since the development of the inner ear was compromised.<h4>Conclusions/significance</h4>Our results reveal that zebrafish β-tectorin is specifically expressed in the zebrafish inner ear, and is important for regulating the development of the zebrafish inner ear. Lack of zebrafish β-tectorin caused severe defects in inner ear formation of otoliths and function.Chung-Hsiang YangChia-Hsiung ChengGen-Der ChenWei-Hao LiaoYi-Chung ChenKai-Yun HuangPung-Pung HwangSheng-Ping L HwangChang-Jen HuangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 8, p e23078 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chung-Hsiang Yang Chia-Hsiung Cheng Gen-Der Chen Wei-Hao Liao Yi-Chung Chen Kai-Yun Huang Pung-Pung Hwang Sheng-Ping L Hwang Chang-Jen Huang Zona pellucida domain-containing protein β-tectorin is crucial for zebrafish proper inner ear development.
description	<h4>Background</h4>The zona pellucida (ZP) domain is part of many extracellular proteins with diverse functions from structural components to receptors. The mammalian β-tectorin is a protein of 336 amino acid residues containing a single ZP domain and a putative signal peptide at the N-terminus of the protein. It is 1 component of a gel-like structure called the tectorial membrane which is involved in transforming sound waves into neuronal signals and is important for normal auditory function. β-Tectorin is specifically expressed in the mammalian and avian inner ear.<h4>Methodology/principal findings</h4>We identified and cloned the gene encoding zebrafish β-tectorin. Through whole-mount in situ hybridization, we demonstrated that β-tectorin messenger RNA was expressed in the otic placode and specialized sensory patch of the inner ear during zebrafish embryonic stages. Morpholino knockdown of zebrafish β-tectorin affected the position and number of otoliths in the ears of morphants. Finally, swimming behaviors of β-tectorin morphants were abnormal since the development of the inner ear was compromised.<h4>Conclusions/significance</h4>Our results reveal that zebrafish β-tectorin is specifically expressed in the zebrafish inner ear, and is important for regulating the development of the zebrafish inner ear. Lack of zebrafish β-tectorin caused severe defects in inner ear formation of otoliths and function.
format	article
author	Chung-Hsiang Yang Chia-Hsiung Cheng Gen-Der Chen Wei-Hao Liao Yi-Chung Chen Kai-Yun Huang Pung-Pung Hwang Sheng-Ping L Hwang Chang-Jen Huang
author_facet	Chung-Hsiang Yang Chia-Hsiung Cheng Gen-Der Chen Wei-Hao Liao Yi-Chung Chen Kai-Yun Huang Pung-Pung Hwang Sheng-Ping L Hwang Chang-Jen Huang
author_sort	Chung-Hsiang Yang
title	Zona pellucida domain-containing protein β-tectorin is crucial for zebrafish proper inner ear development.
title_short	Zona pellucida domain-containing protein β-tectorin is crucial for zebrafish proper inner ear development.
title_full	Zona pellucida domain-containing protein β-tectorin is crucial for zebrafish proper inner ear development.
title_fullStr	Zona pellucida domain-containing protein β-tectorin is crucial for zebrafish proper inner ear development.
title_full_unstemmed	Zona pellucida domain-containing protein β-tectorin is crucial for zebrafish proper inner ear development.
title_sort	zona pellucida domain-containing protein β-tectorin is crucial for zebrafish proper inner ear development.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/360ecf0ff1f447c1b398548de2adbfb8
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Zona pellucida domain-containing protein β-tectorin is crucial for zebrafish proper inner ear development.

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