The Effects of Antifreeze Proteins I and III Supplemented Medium on Cryopreserved Rat Ovaries in Different Durations

Cryopreservation techniques achieved by optimization of the composition and concentration of cryoprotectants not only reduce cryoinjury to ovarian tissue but also enhance survival. Anti-Freeze Proteins (AFPs) have been acknowledged to have positive effects on the survival of oocytes, embryos and ov...

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Autores principales:	Gozde ERKANLI SENTURK, Halil Ibrahim SAYGI
Formato:	article
Lenguaje:	EN
Publicado:	University of Kafkas 2021
Materias:	anti-freeze protein cryopreservation ovary vitrification rat Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/361d5b3f4cf24c4e92d8b850bb596f02
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spelling	oai:doaj.org-article:361d5b3f4cf24c4e92d8b850bb596f022021-12-01T08:20:15ZThe Effects of Antifreeze Proteins I and III Supplemented Medium on Cryopreserved Rat Ovaries in Different Durations10.9775/kvfd.2021.259521300-60451309-2251https://doaj.org/article/361d5b3f4cf24c4e92d8b850bb596f022021-09-01T00:00:00Zhttp://vetdergikafkas.org/uploads/pdf/pdf_KVFD_2841.pdfhttps://doaj.org/toc/1300-6045https://doaj.org/toc/1309-2251Cryopreservation techniques achieved by optimization of the composition and concentration of cryoprotectants not only reduce cryoinjury to ovarian tissue but also enhance survival. Anti-Freeze Proteins (AFPs) have been acknowledged to have positive effects on the survival of oocytes, embryos and ovaries during cryopreservation. AFPs drop the freezing point beneath the balance point of melting by binding ice crystals; restricting their expansion and recrystallization. This study aimed to investigate the protective effects of AFP I and III separately or in combination on the vitrification and warming procedures of ovarian tissues which were vitrified for one week and one month. Ovaries were obtained from rats and randomly assigned to four groups according to the AFP supplements in the media used during freeze/warm procedures: 1) control group (medium without AFP); 2) AFP I; 3) AFP III; 4) AFP I + AFP III groups. The groups were further organized according to the duration of vitrification, 1 week or 1 month. Ovaries were evaluated morphologically by a grading system, TUNEL assay and by transmission electron microscopy. AFP supplementation decreased apoptosis and follicular damage. Supplementation of AFP type I and type III has cryoprotective roles in ovaries which were vitrified for 1 week and 1 month. Additionally, AFP I+AFP III supplementation were shown to have more protection in long term.Gozde ERKANLI SENTURKHalil Ibrahim SAYGIUniversity of Kafkasarticleanti-freeze proteincryopreservationovaryvitrificationratVeterinary medicineSF600-1100ENKafkas Universitesi Veteriner Fakultesi Dergisi, Vol 27, Iss 5, Pp 601-608 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	anti-freeze protein cryopreservation ovary vitrification rat Veterinary medicine SF600-1100
spellingShingle	anti-freeze protein cryopreservation ovary vitrification rat Veterinary medicine SF600-1100 Gozde ERKANLI SENTURK Halil Ibrahim SAYGI The Effects of Antifreeze Proteins I and III Supplemented Medium on Cryopreserved Rat Ovaries in Different Durations
description	Cryopreservation techniques achieved by optimization of the composition and concentration of cryoprotectants not only reduce cryoinjury to ovarian tissue but also enhance survival. Anti-Freeze Proteins (AFPs) have been acknowledged to have positive effects on the survival of oocytes, embryos and ovaries during cryopreservation. AFPs drop the freezing point beneath the balance point of melting by binding ice crystals; restricting their expansion and recrystallization. This study aimed to investigate the protective effects of AFP I and III separately or in combination on the vitrification and warming procedures of ovarian tissues which were vitrified for one week and one month. Ovaries were obtained from rats and randomly assigned to four groups according to the AFP supplements in the media used during freeze/warm procedures: 1) control group (medium without AFP); 2) AFP I; 3) AFP III; 4) AFP I + AFP III groups. The groups were further organized according to the duration of vitrification, 1 week or 1 month. Ovaries were evaluated morphologically by a grading system, TUNEL assay and by transmission electron microscopy. AFP supplementation decreased apoptosis and follicular damage. Supplementation of AFP type I and type III has cryoprotective roles in ovaries which were vitrified for 1 week and 1 month. Additionally, AFP I+AFP III supplementation were shown to have more protection in long term.
format	article
author	Gozde ERKANLI SENTURK Halil Ibrahim SAYGI
author_facet	Gozde ERKANLI SENTURK Halil Ibrahim SAYGI
author_sort	Gozde ERKANLI SENTURK
title	The Effects of Antifreeze Proteins I and III Supplemented Medium on Cryopreserved Rat Ovaries in Different Durations
title_short	The Effects of Antifreeze Proteins I and III Supplemented Medium on Cryopreserved Rat Ovaries in Different Durations
title_full	The Effects of Antifreeze Proteins I and III Supplemented Medium on Cryopreserved Rat Ovaries in Different Durations
title_fullStr	The Effects of Antifreeze Proteins I and III Supplemented Medium on Cryopreserved Rat Ovaries in Different Durations
title_full_unstemmed	The Effects of Antifreeze Proteins I and III Supplemented Medium on Cryopreserved Rat Ovaries in Different Durations
title_sort	effects of antifreeze proteins i and iii supplemented medium on cryopreserved rat ovaries in different durations
publisher	University of Kafkas
publishDate	2021
url	https://doaj.org/article/361d5b3f4cf24c4e92d8b850bb596f02
work_keys_str_mv	AT gozdeerkanlisenturk theeffectsofantifreezeproteinsiandiiisupplementedmediumoncryopreservedratovariesindifferentdurations AT halilibrahimsaygi theeffectsofantifreezeproteinsiandiiisupplementedmediumoncryopreservedratovariesindifferentdurations AT gozdeerkanlisenturk effectsofantifreezeproteinsiandiiisupplementedmediumoncryopreservedratovariesindifferentdurations AT halilibrahimsaygi effectsofantifreezeproteinsiandiiisupplementedmediumoncryopreservedratovariesindifferentdurations
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The Effects of Antifreeze Proteins I and III Supplemented Medium on Cryopreserved Rat Ovaries in Different Durations

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