A Cytochrome B5-Like Heme/Steroid Binding Domain Protein, PlCB5L1, Regulates Mycelial Growth, Pathogenicity and Oxidative Stress Tolerance in Peronophythora litchii

As an electron transport component, cytochrome b5 is an essential component of the Class II cytochrome P450 monooxygenation system and widely present in animals, plants, and fungi. However, the roles of Cyt-b5 domain proteins in pathogenic oomycetes remain unknown. Peronophythora litchii is an oomyc...

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Autores principales:	Wen Li, Peng Li, Xiaofan Zhou, Junjian Situ, Yiming Lin, Jiahui Qiu, Yuling Yuan, Pinggen Xi, Zide Jiang, Guanghui Kong
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Peronophythora litchii cytochrome b5-like heme/steroid binding domain growth pathogenicity oxidative stress Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/3626293b5d2b436bbe3ff25a07d96ff1
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spelling	oai:doaj.org-article:3626293b5d2b436bbe3ff25a07d96ff12021-12-01T01:13:30ZA Cytochrome B5-Like Heme/Steroid Binding Domain Protein, PlCB5L1, Regulates Mycelial Growth, Pathogenicity and Oxidative Stress Tolerance in Peronophythora litchii1664-462X10.3389/fpls.2021.783438https://doaj.org/article/3626293b5d2b436bbe3ff25a07d96ff12021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.783438/fullhttps://doaj.org/toc/1664-462XAs an electron transport component, cytochrome b5 is an essential component of the Class II cytochrome P450 monooxygenation system and widely present in animals, plants, and fungi. However, the roles of Cyt-b5 domain proteins in pathogenic oomycetes remain unknown. Peronophythora litchii is an oomycete pathogen that causes litchi downy blight, the most destructive disease of litchi. In this study, we identified a gene, designated PlCB5L1, that encodes a Cyt-b5 domain protein in P. litchii, and characterized its function. PlCB5L1 is highly expressed in the zoospores, cysts, germinated cysts, and during early stages of infection. PlCB5L1 knockout mutants showed reduced growth rate and β-sitosterol utilization. Importantly, we also found that PlCB5L1 is required for the full pathogenicity of P. litchii. Compared with the wild-type strain, the PlCB5L1 mutants exhibited significantly higher tolerance to SDS and sorbitol, but impaired tolerance to cell wall stress, osmotic stress, and oxidative stress. Further, the expression of genes involved in oxidative stress tolerance, including peroxidase, cytochrome P450, and laccase genes, were down-regulated in PlCB5L1 mutants under oxidative stress. This is the first report that a Cyt-b5 domain protein contributes to the development, stress response, and pathogenicity in plant pathogenic oomycetes.Wen LiPeng LiXiaofan ZhouXiaofan ZhouJunjian SituYiming LinJiahui QiuYuling YuanPinggen XiZide JiangGuanghui KongFrontiers Media S.A.articlePeronophythora litchiicytochrome b5-like heme/steroid binding domaingrowthpathogenicityoxidative stressPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Peronophythora litchii cytochrome b5-like heme/steroid binding domain growth pathogenicity oxidative stress Plant culture SB1-1110
spellingShingle	Peronophythora litchii cytochrome b5-like heme/steroid binding domain growth pathogenicity oxidative stress Plant culture SB1-1110 Wen Li Peng Li Xiaofan Zhou Xiaofan Zhou Junjian Situ Yiming Lin Jiahui Qiu Yuling Yuan Pinggen Xi Zide Jiang Guanghui Kong A Cytochrome B5-Like Heme/Steroid Binding Domain Protein, PlCB5L1, Regulates Mycelial Growth, Pathogenicity and Oxidative Stress Tolerance in Peronophythora litchii
description	As an electron transport component, cytochrome b5 is an essential component of the Class II cytochrome P450 monooxygenation system and widely present in animals, plants, and fungi. However, the roles of Cyt-b5 domain proteins in pathogenic oomycetes remain unknown. Peronophythora litchii is an oomycete pathogen that causes litchi downy blight, the most destructive disease of litchi. In this study, we identified a gene, designated PlCB5L1, that encodes a Cyt-b5 domain protein in P. litchii, and characterized its function. PlCB5L1 is highly expressed in the zoospores, cysts, germinated cysts, and during early stages of infection. PlCB5L1 knockout mutants showed reduced growth rate and β-sitosterol utilization. Importantly, we also found that PlCB5L1 is required for the full pathogenicity of P. litchii. Compared with the wild-type strain, the PlCB5L1 mutants exhibited significantly higher tolerance to SDS and sorbitol, but impaired tolerance to cell wall stress, osmotic stress, and oxidative stress. Further, the expression of genes involved in oxidative stress tolerance, including peroxidase, cytochrome P450, and laccase genes, were down-regulated in PlCB5L1 mutants under oxidative stress. This is the first report that a Cyt-b5 domain protein contributes to the development, stress response, and pathogenicity in plant pathogenic oomycetes.
format	article
author	Wen Li Peng Li Xiaofan Zhou Xiaofan Zhou Junjian Situ Yiming Lin Jiahui Qiu Yuling Yuan Pinggen Xi Zide Jiang Guanghui Kong
author_facet	Wen Li Peng Li Xiaofan Zhou Xiaofan Zhou Junjian Situ Yiming Lin Jiahui Qiu Yuling Yuan Pinggen Xi Zide Jiang Guanghui Kong
author_sort	Wen Li
title	A Cytochrome B5-Like Heme/Steroid Binding Domain Protein, PlCB5L1, Regulates Mycelial Growth, Pathogenicity and Oxidative Stress Tolerance in Peronophythora litchii
title_short	A Cytochrome B5-Like Heme/Steroid Binding Domain Protein, PlCB5L1, Regulates Mycelial Growth, Pathogenicity and Oxidative Stress Tolerance in Peronophythora litchii
title_full	A Cytochrome B5-Like Heme/Steroid Binding Domain Protein, PlCB5L1, Regulates Mycelial Growth, Pathogenicity and Oxidative Stress Tolerance in Peronophythora litchii
title_fullStr	A Cytochrome B5-Like Heme/Steroid Binding Domain Protein, PlCB5L1, Regulates Mycelial Growth, Pathogenicity and Oxidative Stress Tolerance in Peronophythora litchii
title_full_unstemmed	A Cytochrome B5-Like Heme/Steroid Binding Domain Protein, PlCB5L1, Regulates Mycelial Growth, Pathogenicity and Oxidative Stress Tolerance in Peronophythora litchii
title_sort	cytochrome b5-like heme/steroid binding domain protein, plcb5l1, regulates mycelial growth, pathogenicity and oxidative stress tolerance in peronophythora litchii
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/3626293b5d2b436bbe3ff25a07d96ff1
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A Cytochrome B5-Like Heme/Steroid Binding Domain Protein, PlCB5L1, Regulates Mycelial Growth, Pathogenicity and Oxidative Stress Tolerance in Peronophythora litchii

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