Silver nanoparticles affect glucose metabolism in hepatoma cells through production of reactive oxygen species

Mi Jin Lee,1 Seung Jun Lee,1,2,* Su Jin Yun,1,2,* Ji-Young Jang,3 Hangoo Kang,3 Kyongmin Kim,1,2 In-Hong Choi,3,4 Sun Park1,2 1Department of Microbiology, Ajou University School of Medicine, 2Department of Biomedical Sciences, The Graduate School, Ajou University, Suwon, 3Department of Microbiology...

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Autores principales:	Lee MJ, Lee SJ, Yun SJ, Jang JY, Kang HG, Kim K, Choi IH, Park S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	metal nanoparticles carbohydrate metabolism reactive oxygen species hepatocellular carcinoma Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/362a1f36c430478e9e951dc3d91a7504
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spelling	oai:doaj.org-article:362a1f36c430478e9e951dc3d91a75042021-12-02T00:38:52ZSilver nanoparticles affect glucose metabolism in hepatoma cells through production of reactive oxygen species1178-2013https://doaj.org/article/362a1f36c430478e9e951dc3d91a75042015-12-01T00:00:00Zhttps://www.dovepress.com/silver-nanoparticles-affect-glucose-metabolism-in-hepatoma-cells-throu-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Mi Jin Lee,1 Seung Jun Lee,1,2,* Su Jin Yun,1,2,* Ji-Young Jang,3 Hangoo Kang,3 Kyongmin Kim,1,2 In-Hong Choi,3,4 Sun Park1,2 1Department of Microbiology, Ajou University School of Medicine, 2Department of Biomedical Sciences, The Graduate School, Ajou University, Suwon, 3Department of Microbiology, The Institute for Immunology and Immunological Diseases, Yonsei University College of Medicine, Seoul, Korea; 4Brain Korea 21 Plus Project for Medical Sciences, Yonsei University College of Medicine, Seoul, Korea *These authors contributed equally to this work Abstract: The silver nanoparticle (AgNP) is a candidate for anticancer therapy because of its effects on cell survival and signaling. Although numerous reports are available regarding their effect on cell death, the effect of AgNPs on metabolism is not well understood. In this study, we investigated the effect of AgNPs on glucose metabolism in hepatoma cell lines. Lactate release from both HepG2 and Huh7 cells was reduced with 5 nm AgNPs as early as 1 hour after treatment, when cell death did not occur. Treatment with 5 nm AgNPs decreased glucose consumption in HepG2 cells but not in Huh7 cells. Treatment with 5 nm AgNPs reduced nuclear factor erythroid 2-like 2 expression in both cell types without affecting its activation at the early time points after AgNPs’ treatment. Increased reactive oxygen species (ROS) production was detected 1 hour after 5 nm AgNPs’ treatment, and lactate release was restored in the presence of an ROS scavenger. Our results suggest that 5 nm AgNPs affect glucose metabolism by producing ROS. Keywords: metal nanoparticles, carbohydrate metabolism, lactic acid, cytotoxicity Lee MJLee SJYun SJJang JYKang HGKim KChoi IHPark SDove Medical Pressarticlemetal nanoparticlescarbohydrate metabolismreactive oxygen specieshepatocellular carcinomaMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss Issue 1, Pp 55-68 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	metal nanoparticles carbohydrate metabolism reactive oxygen species hepatocellular carcinoma Medicine (General) R5-920
spellingShingle	metal nanoparticles carbohydrate metabolism reactive oxygen species hepatocellular carcinoma Medicine (General) R5-920 Lee MJ Lee SJ Yun SJ Jang JY Kang HG Kim K Choi IH Park S Silver nanoparticles affect glucose metabolism in hepatoma cells through production of reactive oxygen species
description	Mi Jin Lee,1 Seung Jun Lee,1,2,* Su Jin Yun,1,2,* Ji-Young Jang,3 Hangoo Kang,3 Kyongmin Kim,1,2 In-Hong Choi,3,4 Sun Park1,2 1Department of Microbiology, Ajou University School of Medicine, 2Department of Biomedical Sciences, The Graduate School, Ajou University, Suwon, 3Department of Microbiology, The Institute for Immunology and Immunological Diseases, Yonsei University College of Medicine, Seoul, Korea; 4Brain Korea 21 Plus Project for Medical Sciences, Yonsei University College of Medicine, Seoul, Korea *These authors contributed equally to this work Abstract: The silver nanoparticle (AgNP) is a candidate for anticancer therapy because of its effects on cell survival and signaling. Although numerous reports are available regarding their effect on cell death, the effect of AgNPs on metabolism is not well understood. In this study, we investigated the effect of AgNPs on glucose metabolism in hepatoma cell lines. Lactate release from both HepG2 and Huh7 cells was reduced with 5 nm AgNPs as early as 1 hour after treatment, when cell death did not occur. Treatment with 5 nm AgNPs decreased glucose consumption in HepG2 cells but not in Huh7 cells. Treatment with 5 nm AgNPs reduced nuclear factor erythroid 2-like 2 expression in both cell types without affecting its activation at the early time points after AgNPs’ treatment. Increased reactive oxygen species (ROS) production was detected 1 hour after 5 nm AgNPs’ treatment, and lactate release was restored in the presence of an ROS scavenger. Our results suggest that 5 nm AgNPs affect glucose metabolism by producing ROS. Keywords: metal nanoparticles, carbohydrate metabolism, lactic acid, cytotoxicity
format	article
author	Lee MJ Lee SJ Yun SJ Jang JY Kang HG Kim K Choi IH Park S
author_facet	Lee MJ Lee SJ Yun SJ Jang JY Kang HG Kim K Choi IH Park S
author_sort	Lee MJ
title	Silver nanoparticles affect glucose metabolism in hepatoma cells through production of reactive oxygen species
title_short	Silver nanoparticles affect glucose metabolism in hepatoma cells through production of reactive oxygen species
title_full	Silver nanoparticles affect glucose metabolism in hepatoma cells through production of reactive oxygen species
title_fullStr	Silver nanoparticles affect glucose metabolism in hepatoma cells through production of reactive oxygen species
title_full_unstemmed	Silver nanoparticles affect glucose metabolism in hepatoma cells through production of reactive oxygen species
title_sort	silver nanoparticles affect glucose metabolism in hepatoma cells through production of reactive oxygen species
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/362a1f36c430478e9e951dc3d91a7504
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_version_	1718403546921041920

Silver nanoparticles affect glucose metabolism in hepatoma cells through production of reactive oxygen species

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