Antioxidant activity of inulin and its role in the prevention of human colonic muscle cell impairment induced by lipopolysaccharide mucosal exposure.

<h4>Background</h4>Fructans, such as inulin, are dietary fibers which stimulate gastro-intestinal (GI) function acting as prebiotics. Lipopolysaccharide (LPS) impairs GI motility, through production of reactive oxygen species. The antioxidant activity of various fructans was tested and t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Valentina Pasqualetti, Annamaria Altomare, Michele Pier Luca Guarino, Vittoria Locato, Silvia Cocca, Sara Cimini, Rossella Palma, Rossana Alloni, Laura De Gara, Michele Cicala
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3631ac633a8e4b9caba29f27068ebfaa
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3631ac633a8e4b9caba29f27068ebfaa
record_format	dspace
spelling	oai:doaj.org-article:3631ac633a8e4b9caba29f27068ebfaa2021-11-18T08:18:54ZAntioxidant activity of inulin and its role in the prevention of human colonic muscle cell impairment induced by lipopolysaccharide mucosal exposure.1932-620310.1371/journal.pone.0098031https://doaj.org/article/3631ac633a8e4b9caba29f27068ebfaa2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24837182/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Fructans, such as inulin, are dietary fibers which stimulate gastro-intestinal (GI) function acting as prebiotics. Lipopolysaccharide (LPS) impairs GI motility, through production of reactive oxygen species. The antioxidant activity of various fructans was tested and the protective effect of inulin on colonic smooth muscle cell (SMC) impairment, induced by exposure of human mucosa to LPS, was assessed in an ex vivo experimental model.<h4>Methods</h4>The antioxidant capacity of fructans was measured in an in vitro system that simulates cooking and digestion processes. Human colonic mucosa and submucosa, obtained from disease-free margins of resected segments for cancer, were sealed between two chambers, with the mucosal side facing upwards with Krebs solution with or without purified LPS from a pathogenic strain of Escherichia coli (O111:B4) and inulin (Frutafit IQ), and the submucosal side facing downwards into Krebs solution. The solutions on the submucosal side were collected following mucosal exposure to Krebs in the absence (N-undernatant) or presence of LPS (LPS-undernatant) or LPS+inulin (LPS+INU-undernatant). Undernatants were tested for their antioxidant activity and the effects on SMCs contractility. Inulin protective effects on mucosa and submucosa layers were assessed measuring the protein oxidation level in the experimental conditions analyzed.<h4>Results</h4>Antioxidant activity of inulin, which was significantly higher compared to simple sugars, remained unaltered despite cooking and digestion processes. Inulin protected the mucosal and submucosal layers against protein oxidation. Following exposure to LPS-undernatant, a significant decrease in maximal acetylcholine (Ach)-induced contraction was observed when compared to the contraction induced in cells incubated with the N-undernatant (4±1% vs 25±5% respectively, P<0.005) and this effect was completely prevented by pre-incubation of LPS with Inulin (35±5%).<h4>Conclusions</h4>Inulin protects the human colon mucosa from LPS-induced damage and this effect appears to be related to the protective effect of inulin against LPS-induced oxidative stress.Valentina PasqualettiAnnamaria AltomareMichele Pier Luca GuarinoVittoria LocatoSilvia CoccaSara CiminiRossella PalmaRossana AlloniLaura De GaraMichele CicalaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 5, p e98031 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Valentina Pasqualetti Annamaria Altomare Michele Pier Luca Guarino Vittoria Locato Silvia Cocca Sara Cimini Rossella Palma Rossana Alloni Laura De Gara Michele Cicala Antioxidant activity of inulin and its role in the prevention of human colonic muscle cell impairment induced by lipopolysaccharide mucosal exposure.
description	<h4>Background</h4>Fructans, such as inulin, are dietary fibers which stimulate gastro-intestinal (GI) function acting as prebiotics. Lipopolysaccharide (LPS) impairs GI motility, through production of reactive oxygen species. The antioxidant activity of various fructans was tested and the protective effect of inulin on colonic smooth muscle cell (SMC) impairment, induced by exposure of human mucosa to LPS, was assessed in an ex vivo experimental model.<h4>Methods</h4>The antioxidant capacity of fructans was measured in an in vitro system that simulates cooking and digestion processes. Human colonic mucosa and submucosa, obtained from disease-free margins of resected segments for cancer, were sealed between two chambers, with the mucosal side facing upwards with Krebs solution with or without purified LPS from a pathogenic strain of Escherichia coli (O111:B4) and inulin (Frutafit IQ), and the submucosal side facing downwards into Krebs solution. The solutions on the submucosal side were collected following mucosal exposure to Krebs in the absence (N-undernatant) or presence of LPS (LPS-undernatant) or LPS+inulin (LPS+INU-undernatant). Undernatants were tested for their antioxidant activity and the effects on SMCs contractility. Inulin protective effects on mucosa and submucosa layers were assessed measuring the protein oxidation level in the experimental conditions analyzed.<h4>Results</h4>Antioxidant activity of inulin, which was significantly higher compared to simple sugars, remained unaltered despite cooking and digestion processes. Inulin protected the mucosal and submucosal layers against protein oxidation. Following exposure to LPS-undernatant, a significant decrease in maximal acetylcholine (Ach)-induced contraction was observed when compared to the contraction induced in cells incubated with the N-undernatant (4±1% vs 25±5% respectively, P<0.005) and this effect was completely prevented by pre-incubation of LPS with Inulin (35±5%).<h4>Conclusions</h4>Inulin protects the human colon mucosa from LPS-induced damage and this effect appears to be related to the protective effect of inulin against LPS-induced oxidative stress.
format	article
author	Valentina Pasqualetti Annamaria Altomare Michele Pier Luca Guarino Vittoria Locato Silvia Cocca Sara Cimini Rossella Palma Rossana Alloni Laura De Gara Michele Cicala
author_facet	Valentina Pasqualetti Annamaria Altomare Michele Pier Luca Guarino Vittoria Locato Silvia Cocca Sara Cimini Rossella Palma Rossana Alloni Laura De Gara Michele Cicala
author_sort	Valentina Pasqualetti
title	Antioxidant activity of inulin and its role in the prevention of human colonic muscle cell impairment induced by lipopolysaccharide mucosal exposure.
title_short	Antioxidant activity of inulin and its role in the prevention of human colonic muscle cell impairment induced by lipopolysaccharide mucosal exposure.
title_full	Antioxidant activity of inulin and its role in the prevention of human colonic muscle cell impairment induced by lipopolysaccharide mucosal exposure.
title_fullStr	Antioxidant activity of inulin and its role in the prevention of human colonic muscle cell impairment induced by lipopolysaccharide mucosal exposure.
title_full_unstemmed	Antioxidant activity of inulin and its role in the prevention of human colonic muscle cell impairment induced by lipopolysaccharide mucosal exposure.
title_sort	antioxidant activity of inulin and its role in the prevention of human colonic muscle cell impairment induced by lipopolysaccharide mucosal exposure.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/3631ac633a8e4b9caba29f27068ebfaa
work_keys_str_mv	AT valentinapasqualetti antioxidantactivityofinulinanditsroleinthepreventionofhumancolonicmusclecellimpairmentinducedbylipopolysaccharidemucosalexposure AT annamariaaltomare antioxidantactivityofinulinanditsroleinthepreventionofhumancolonicmusclecellimpairmentinducedbylipopolysaccharidemucosalexposure AT michelepierlucaguarino antioxidantactivityofinulinanditsroleinthepreventionofhumancolonicmusclecellimpairmentinducedbylipopolysaccharidemucosalexposure AT vittorialocato antioxidantactivityofinulinanditsroleinthepreventionofhumancolonicmusclecellimpairmentinducedbylipopolysaccharidemucosalexposure AT silviacocca antioxidantactivityofinulinanditsroleinthepreventionofhumancolonicmusclecellimpairmentinducedbylipopolysaccharidemucosalexposure AT saracimini antioxidantactivityofinulinanditsroleinthepreventionofhumancolonicmusclecellimpairmentinducedbylipopolysaccharidemucosalexposure AT rossellapalma antioxidantactivityofinulinanditsroleinthepreventionofhumancolonicmusclecellimpairmentinducedbylipopolysaccharidemucosalexposure AT rossanaalloni antioxidantactivityofinulinanditsroleinthepreventionofhumancolonicmusclecellimpairmentinducedbylipopolysaccharidemucosalexposure AT lauradegara antioxidantactivityofinulinanditsroleinthepreventionofhumancolonicmusclecellimpairmentinducedbylipopolysaccharidemucosalexposure AT michelecicala antioxidantactivityofinulinanditsroleinthepreventionofhumancolonicmusclecellimpairmentinducedbylipopolysaccharidemucosalexposure
_version_	1718421914862485504

Antioxidant activity of inulin and its role in the prevention of human colonic muscle cell impairment induced by lipopolysaccharide mucosal exposure.

Ejemplares similares