Protein allocation and utilization in the versatile chemolithoautotroph Cupriavidus necator

Bacteria must balance the different needs for substrate assimilation, growth functions, and resilience in order to thrive in their environment. Of all cellular macromolecules, the bacterial proteome is by far the most important resource and its size is limited. Here, we investigated how the highly v...

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Autores principales:	Michael Jahn, Nick Crang, Markus Janasch, Andreas Hober, Björn Forsström, Kyle Kimler, Alexander Mattausch, Qi Chen, Johannes Asplund-Samuelsson, Elton Paul Hudson
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	Cupriavidus necator Ralstonia eutropha resource balance analysis substrate limitation co2 fixation gene fitness Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3631e860f20c479ab63f583005d0297c
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spelling	oai:doaj.org-article:3631e860f20c479ab63f583005d0297c2021-11-16T17:58:41ZProtein allocation and utilization in the versatile chemolithoautotroph Cupriavidus necator10.7554/eLife.690192050-084Xe69019https://doaj.org/article/3631e860f20c479ab63f583005d0297c2021-11-01T00:00:00Zhttps://elifesciences.org/articles/69019https://doaj.org/toc/2050-084XBacteria must balance the different needs for substrate assimilation, growth functions, and resilience in order to thrive in their environment. Of all cellular macromolecules, the bacterial proteome is by far the most important resource and its size is limited. Here, we investigated how the highly versatile 'knallgas' bacterium Cupriavidus necator reallocates protein resources when grown on different limiting substrates and with different growth rates. We determined protein quantity by mass spectrometry and estimated enzyme utilization by resource balance analysis modeling. We found that C. necator invests a large fraction of its proteome in functions that are hardly utilized. Of the enzymes that are utilized, many are present in excess abundance. One prominent example is the strong expression of CBB cycle genes such as Rubisco during growth on fructose. Modeling and mutant competition experiments suggest that CO2-reassimilation through Rubisco does not provide a fitness benefit for heterotrophic growth, but is rather an investment in readiness for autotrophy.Michael JahnNick CrangMarkus JanaschAndreas HoberBjörn ForsströmKyle KimlerAlexander MattauschQi ChenJohannes Asplund-SamuelssonElton Paul HudsoneLife Sciences Publications LtdarticleCupriavidus necatorRalstonia eutropharesource balance analysissubstrate limitationco2 fixationgene fitnessMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cupriavidus necator Ralstonia eutropha resource balance analysis substrate limitation co2 fixation gene fitness Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	Cupriavidus necator Ralstonia eutropha resource balance analysis substrate limitation co2 fixation gene fitness Medicine R Science Q Biology (General) QH301-705.5 Michael Jahn Nick Crang Markus Janasch Andreas Hober Björn Forsström Kyle Kimler Alexander Mattausch Qi Chen Johannes Asplund-Samuelsson Elton Paul Hudson Protein allocation and utilization in the versatile chemolithoautotroph Cupriavidus necator
description	Bacteria must balance the different needs for substrate assimilation, growth functions, and resilience in order to thrive in their environment. Of all cellular macromolecules, the bacterial proteome is by far the most important resource and its size is limited. Here, we investigated how the highly versatile 'knallgas' bacterium Cupriavidus necator reallocates protein resources when grown on different limiting substrates and with different growth rates. We determined protein quantity by mass spectrometry and estimated enzyme utilization by resource balance analysis modeling. We found that C. necator invests a large fraction of its proteome in functions that are hardly utilized. Of the enzymes that are utilized, many are present in excess abundance. One prominent example is the strong expression of CBB cycle genes such as Rubisco during growth on fructose. Modeling and mutant competition experiments suggest that CO2-reassimilation through Rubisco does not provide a fitness benefit for heterotrophic growth, but is rather an investment in readiness for autotrophy.
format	article
author	Michael Jahn Nick Crang Markus Janasch Andreas Hober Björn Forsström Kyle Kimler Alexander Mattausch Qi Chen Johannes Asplund-Samuelsson Elton Paul Hudson
author_facet	Michael Jahn Nick Crang Markus Janasch Andreas Hober Björn Forsström Kyle Kimler Alexander Mattausch Qi Chen Johannes Asplund-Samuelsson Elton Paul Hudson
author_sort	Michael Jahn
title	Protein allocation and utilization in the versatile chemolithoautotroph Cupriavidus necator
title_short	Protein allocation and utilization in the versatile chemolithoautotroph Cupriavidus necator
title_full	Protein allocation and utilization in the versatile chemolithoautotroph Cupriavidus necator
title_fullStr	Protein allocation and utilization in the versatile chemolithoautotroph Cupriavidus necator
title_full_unstemmed	Protein allocation and utilization in the versatile chemolithoautotroph Cupriavidus necator
title_sort	protein allocation and utilization in the versatile chemolithoautotroph cupriavidus necator
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/3631e860f20c479ab63f583005d0297c
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Protein allocation and utilization in the versatile chemolithoautotroph Cupriavidus necator

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