Morphological and molecular identificationof fungal pathogens causing gummosis disease of Citrus spp. in Indonesia

Henuk JBD, Sinaga MS, Hidayat SH. 2017. Morphological and molecular identificationof fungal pathogens causing gummosis disease of Citrus spp. in Indonesia. Biodiversitas 18: 1100-1108. Gummosis is one of the most important diseases of citrus. The disease is caused by several species of Phytophthora,...

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Autores principales: JULINDA B.D. HENUK, MEITY S. SINAGA, SRI H. HIDAYAT
Formato: article
Lenguaje:EN
Publicado: MBI & UNS Solo 2017
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spelling oai:doaj.org-article:363cfcb4abd642f681a625466ddfe2162021-11-16T13:56:34ZMorphological and molecular identificationof fungal pathogens causing gummosis disease of Citrus spp. in Indonesia1412-033X2085-472210.13057/biodiv/d180330https://doaj.org/article/363cfcb4abd642f681a625466ddfe2162017-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2029https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Henuk JBD, Sinaga MS, Hidayat SH. 2017. Morphological and molecular identificationof fungal pathogens causing gummosis disease of Citrus spp. in Indonesia. Biodiversitas 18: 1100-1108. Gummosis is one of the most important diseases of citrus. The disease is caused by several species of Phytophthora, i.e. P. nicotianae, P. palmivora, P. citrophthora; as well as Lasiodiplodia theobromae (synonyms: Botryodiplodia theobromae and Diplodia natalensis; teleomorph Botryosphaeria rhodina). The objective of this study was to identify the isolates of fungal pathogens, i.e. Phytophthora spp. and B. theobromae and/or D. natalensis from several different areas of citrus central production in Indonesia based on their morphological and molecular characteristics. This study included two activities, i.e. (i) collection, isolation, and identification of pathogenic fungi from infected tissues, basal stem roots, and soil surface and rhizosphere; and (ii) extraction of genomic DNA and amplification of DNA fragments using PCR technique for molecular characterization. Results of morphological characterization and DNA sequence analysis revealed that gummosis was caused by Botryodiplodia theobromae Pat. (Teleomorph Botryosphaeria rhodina (Cooke) Arx.)), and P. citrophthora.JULINDA B.D. HENUKMEITY S. SINAGASRI H. HIDAYATMBI & UNS Soloarticlecitrusgummosismorphologymolecular characteristicsBiology (General)QH301-705.5ENBiodiversitas, Vol 18, Iss 3, Pp 1100-1108 (2017)
institution DOAJ
collection DOAJ
language EN
topic citrus
gummosis
morphology
molecular characteristics
Biology (General)
QH301-705.5
spellingShingle citrus
gummosis
morphology
molecular characteristics
Biology (General)
QH301-705.5
JULINDA B.D. HENUK
MEITY S. SINAGA
SRI H. HIDAYAT
Morphological and molecular identificationof fungal pathogens causing gummosis disease of Citrus spp. in Indonesia
description Henuk JBD, Sinaga MS, Hidayat SH. 2017. Morphological and molecular identificationof fungal pathogens causing gummosis disease of Citrus spp. in Indonesia. Biodiversitas 18: 1100-1108. Gummosis is one of the most important diseases of citrus. The disease is caused by several species of Phytophthora, i.e. P. nicotianae, P. palmivora, P. citrophthora; as well as Lasiodiplodia theobromae (synonyms: Botryodiplodia theobromae and Diplodia natalensis; teleomorph Botryosphaeria rhodina). The objective of this study was to identify the isolates of fungal pathogens, i.e. Phytophthora spp. and B. theobromae and/or D. natalensis from several different areas of citrus central production in Indonesia based on their morphological and molecular characteristics. This study included two activities, i.e. (i) collection, isolation, and identification of pathogenic fungi from infected tissues, basal stem roots, and soil surface and rhizosphere; and (ii) extraction of genomic DNA and amplification of DNA fragments using PCR technique for molecular characterization. Results of morphological characterization and DNA sequence analysis revealed that gummosis was caused by Botryodiplodia theobromae Pat. (Teleomorph Botryosphaeria rhodina (Cooke) Arx.)), and P. citrophthora.
format article
author JULINDA B.D. HENUK
MEITY S. SINAGA
SRI H. HIDAYAT
author_facet JULINDA B.D. HENUK
MEITY S. SINAGA
SRI H. HIDAYAT
author_sort JULINDA B.D. HENUK
title Morphological and molecular identificationof fungal pathogens causing gummosis disease of Citrus spp. in Indonesia
title_short Morphological and molecular identificationof fungal pathogens causing gummosis disease of Citrus spp. in Indonesia
title_full Morphological and molecular identificationof fungal pathogens causing gummosis disease of Citrus spp. in Indonesia
title_fullStr Morphological and molecular identificationof fungal pathogens causing gummosis disease of Citrus spp. in Indonesia
title_full_unstemmed Morphological and molecular identificationof fungal pathogens causing gummosis disease of Citrus spp. in Indonesia
title_sort morphological and molecular identificationof fungal pathogens causing gummosis disease of citrus spp. in indonesia
publisher MBI & UNS Solo
publishDate 2017
url https://doaj.org/article/363cfcb4abd642f681a625466ddfe216
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AT meityssinaga morphologicalandmolecularidentificationoffungalpathogenscausinggummosisdiseaseofcitrussppinindonesia
AT srihhidayat morphologicalandmolecularidentificationoffungalpathogenscausinggummosisdiseaseofcitrussppinindonesia
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