Epigenetic signatures associated with different levels of differentiation potential in human stem cells.

<h4>Background</h4>The therapeutic use of multipotent stem cells depends on their differentiation potential, which has been shown to be variable for different populations. These differences are likely to be the result of key changes in their epigenetic profiles.<h4>Methodology/prin...

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Autores principales:	Pablo Aranda, Xabier Agirre, Esteban Ballestar, Enrique J Andreu, José Román-Gómez, Inés Prieto, José Ignacio Martín-Subero, Juan Cruz Cigudosa, Reiner Siebert, Manel Esteller, Felipe Prosper
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/363f57de711646819f927021eddd705e
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spelling	oai:doaj.org-article:363f57de711646819f927021eddd705e2021-11-25T06:28:09ZEpigenetic signatures associated with different levels of differentiation potential in human stem cells.1932-620310.1371/journal.pone.0007809https://doaj.org/article/363f57de711646819f927021eddd705e2009-11-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19915669/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The therapeutic use of multipotent stem cells depends on their differentiation potential, which has been shown to be variable for different populations. These differences are likely to be the result of key changes in their epigenetic profiles.<h4>Methodology/principal findings</h4>to address this issue, we have investigated the levels of epigenetic regulation in well characterized populations of pluripotent embryonic stem cells (ESC) and multipotent adult stem cells (ASC) at the trancriptome, methylome, histone modification and microRNA levels. Differences in gene expression profiles allowed classification of stem cells into three separate populations including ESC, multipotent adult progenitor cells (MAPC) and mesenchymal stromal cells (MSC). The analysis of the PcG repressive marks, histone modifications and gene promoter methylation of differentiation and pluripotency genes demonstrated that stem cell populations with a wider differentiation potential (ESC and MAPC) showed stronger representation of epigenetic repressive marks in differentiation genes and that this epigenetic signature was progressively lost with restriction of stem cell potential. Our analysis of microRNA established specific microRNA signatures suggesting specific microRNAs involved in regulation of pluripotent and differentiation genes.<h4>Conclusions/significance</h4>Our study leads us to propose a model where the level of epigenetic regulation, as a combination of DNA methylation and histone modification marks, at differentiation genes defines degrees of differentiation potential from progenitor and multipotent stem cells to pluripotent stem cells.Pablo ArandaXabier AgirreEsteban BallestarEnrique J AndreuJosé Román-GómezInés PrietoJosé Ignacio Martín-SuberoJuan Cruz CigudosaReiner SiebertManel EstellerFelipe ProsperPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 11, p e7809 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pablo Aranda Xabier Agirre Esteban Ballestar Enrique J Andreu José Román-Gómez Inés Prieto José Ignacio Martín-Subero Juan Cruz Cigudosa Reiner Siebert Manel Esteller Felipe Prosper Epigenetic signatures associated with different levels of differentiation potential in human stem cells.
description	<h4>Background</h4>The therapeutic use of multipotent stem cells depends on their differentiation potential, which has been shown to be variable for different populations. These differences are likely to be the result of key changes in their epigenetic profiles.<h4>Methodology/principal findings</h4>to address this issue, we have investigated the levels of epigenetic regulation in well characterized populations of pluripotent embryonic stem cells (ESC) and multipotent adult stem cells (ASC) at the trancriptome, methylome, histone modification and microRNA levels. Differences in gene expression profiles allowed classification of stem cells into three separate populations including ESC, multipotent adult progenitor cells (MAPC) and mesenchymal stromal cells (MSC). The analysis of the PcG repressive marks, histone modifications and gene promoter methylation of differentiation and pluripotency genes demonstrated that stem cell populations with a wider differentiation potential (ESC and MAPC) showed stronger representation of epigenetic repressive marks in differentiation genes and that this epigenetic signature was progressively lost with restriction of stem cell potential. Our analysis of microRNA established specific microRNA signatures suggesting specific microRNAs involved in regulation of pluripotent and differentiation genes.<h4>Conclusions/significance</h4>Our study leads us to propose a model where the level of epigenetic regulation, as a combination of DNA methylation and histone modification marks, at differentiation genes defines degrees of differentiation potential from progenitor and multipotent stem cells to pluripotent stem cells.
format	article
author	Pablo Aranda Xabier Agirre Esteban Ballestar Enrique J Andreu José Román-Gómez Inés Prieto José Ignacio Martín-Subero Juan Cruz Cigudosa Reiner Siebert Manel Esteller Felipe Prosper
author_facet	Pablo Aranda Xabier Agirre Esteban Ballestar Enrique J Andreu José Román-Gómez Inés Prieto José Ignacio Martín-Subero Juan Cruz Cigudosa Reiner Siebert Manel Esteller Felipe Prosper
author_sort	Pablo Aranda
title	Epigenetic signatures associated with different levels of differentiation potential in human stem cells.
title_short	Epigenetic signatures associated with different levels of differentiation potential in human stem cells.
title_full	Epigenetic signatures associated with different levels of differentiation potential in human stem cells.
title_fullStr	Epigenetic signatures associated with different levels of differentiation potential in human stem cells.
title_full_unstemmed	Epigenetic signatures associated with different levels of differentiation potential in human stem cells.
title_sort	epigenetic signatures associated with different levels of differentiation potential in human stem cells.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/363f57de711646819f927021eddd705e
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Epigenetic signatures associated with different levels of differentiation potential in human stem cells.

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