CD8αα+T cells exert a pro‐inflammatory role in patients with psoriasis

Abstract Background Psoriasis is a common chronic inflammatory disease caused by excessive activation of CD4+T cells, including Th17, Th1 and Th22. The role of CD8+T cells in psoriasis pathogenesis remains poorly understood. Aim To identify the phenotype of CD8+T cells in patients with psoriasis and...

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Autores principales:	Y. Y. Zhang, Y. T. Lin, L. Wang, X. W. Sun, E. L. Dang, K. Xue, W. G. Zhang, K. M. Zhang, G. Wang, B. Li
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	Dermatology RL1-803
Acceso en línea:	https://doaj.org/article/36451d0c06cf4234a85a3f46832ae74f
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spelling	oai:doaj.org-article:36451d0c06cf4234a85a3f46832ae74f2021-12-02T11:13:08ZCD8αα+T cells exert a pro‐inflammatory role in patients with psoriasis2690-442X10.1002/ski2.64https://doaj.org/article/36451d0c06cf4234a85a3f46832ae74f2021-12-01T00:00:00Zhttps://doi.org/10.1002/ski2.64https://doaj.org/toc/2690-442XAbstract Background Psoriasis is a common chronic inflammatory disease caused by excessive activation of CD4+T cells, including Th17, Th1 and Th22. The role of CD8+T cells in psoriasis pathogenesis remains poorly understood. Aim To identify the phenotype of CD8+T cells in patients with psoriasis and to investigate its role in the formation of lesions. Methods The phenotype of CD8+T cells in psoriatic lesions was detected by immunofluorescence staining. Flow cytometry was performed to detect their phenotype in peripheral blood. Thereafter, coculture of CD8αα+T cells with autogenous CD4+T cells was performed to investigate the function of CD8αα+T cells in patients with psoriasis. Finally, pro‐inflammatory factors produced by CD8αα+T cells were examined by immunofluorescence staining and flow cytometry. Results Compared to the CD8αβ+T cells, CD8αα+T cell infiltration in psoriatic lesions markedly increased. Moreover, epidermal CD8αα+T cells exhibited tissue‐resident memory T cells (TRM) phenotypes and dermal CD8αα+T cells exhibited effector memory (TEM) phenotypes in psoriatic lesions. Additionally, we found that CD8αα+T cells from patients with psoriasis did not express the markers of regulatory T cells and could promote the proliferation of CD4+T effector cells and produce interleukin‐17 and interferon‐γ. Conclusions Our findings demonstrate that CD8αα+T cells contribute to the pathogenesis of psoriasis by producing pro‐inflammatory factors.Y. Y. ZhangY. T. LinL. WangX. W. SunE. L. DangK. XueW. G. ZhangK. M. ZhangG. WangB. LiWileyarticleDermatologyRL1-803ENSkin Health and Disease, Vol 1, Iss 4, Pp n/a-n/a (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Dermatology RL1-803
spellingShingle	Dermatology RL1-803 Y. Y. Zhang Y. T. Lin L. Wang X. W. Sun E. L. Dang K. Xue W. G. Zhang K. M. Zhang G. Wang B. Li CD8αα+T cells exert a pro‐inflammatory role in patients with psoriasis
description	Abstract Background Psoriasis is a common chronic inflammatory disease caused by excessive activation of CD4+T cells, including Th17, Th1 and Th22. The role of CD8+T cells in psoriasis pathogenesis remains poorly understood. Aim To identify the phenotype of CD8+T cells in patients with psoriasis and to investigate its role in the formation of lesions. Methods The phenotype of CD8+T cells in psoriatic lesions was detected by immunofluorescence staining. Flow cytometry was performed to detect their phenotype in peripheral blood. Thereafter, coculture of CD8αα+T cells with autogenous CD4+T cells was performed to investigate the function of CD8αα+T cells in patients with psoriasis. Finally, pro‐inflammatory factors produced by CD8αα+T cells were examined by immunofluorescence staining and flow cytometry. Results Compared to the CD8αβ+T cells, CD8αα+T cell infiltration in psoriatic lesions markedly increased. Moreover, epidermal CD8αα+T cells exhibited tissue‐resident memory T cells (TRM) phenotypes and dermal CD8αα+T cells exhibited effector memory (TEM) phenotypes in psoriatic lesions. Additionally, we found that CD8αα+T cells from patients with psoriasis did not express the markers of regulatory T cells and could promote the proliferation of CD4+T effector cells and produce interleukin‐17 and interferon‐γ. Conclusions Our findings demonstrate that CD8αα+T cells contribute to the pathogenesis of psoriasis by producing pro‐inflammatory factors.
format	article
author	Y. Y. Zhang Y. T. Lin L. Wang X. W. Sun E. L. Dang K. Xue W. G. Zhang K. M. Zhang G. Wang B. Li
author_facet	Y. Y. Zhang Y. T. Lin L. Wang X. W. Sun E. L. Dang K. Xue W. G. Zhang K. M. Zhang G. Wang B. Li
author_sort	Y. Y. Zhang
title	CD8αα+T cells exert a pro‐inflammatory role in patients with psoriasis
title_short	CD8αα+T cells exert a pro‐inflammatory role in patients with psoriasis
title_full	CD8αα+T cells exert a pro‐inflammatory role in patients with psoriasis
title_fullStr	CD8αα+T cells exert a pro‐inflammatory role in patients with psoriasis
title_full_unstemmed	CD8αα+T cells exert a pro‐inflammatory role in patients with psoriasis
title_sort	cd8αα+t cells exert a pro‐inflammatory role in patients with psoriasis
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/36451d0c06cf4234a85a3f46832ae74f
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CD8αα+T cells exert a pro‐inflammatory role in patients with psoriasis

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