Proteins related to ictogenesis and seizure clustering in chronic epilepsy

Abstract Seizure clustering is a common phenomenon in epilepsy. Protein expression profiles during a seizure cluster might reflect the pathomechanism underlying ictogenesis. We performed proteomic analyses to identify proteins with a specific temporal expression pattern in cluster phases and to demo...

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Autores principales:	Woo-Jin Lee, Jangsup Moon, Jung-Ah Lim, Daejong Jeon, Jung-Suk Yoo, Dong-Kyu Park, Dohyun Han, Soon-Tae Lee, Keun-Hwa Jung, Kyung-Il Park, Sang Kun Lee, Kon Chu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/364c50be800d4201bee1cb4de9140637
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spelling	oai:doaj.org-article:364c50be800d4201bee1cb4de91406372021-11-08T10:55:22ZProteins related to ictogenesis and seizure clustering in chronic epilepsy10.1038/s41598-021-00956-62045-2322https://doaj.org/article/364c50be800d4201bee1cb4de91406372021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-00956-6https://doaj.org/toc/2045-2322Abstract Seizure clustering is a common phenomenon in epilepsy. Protein expression profiles during a seizure cluster might reflect the pathomechanism underlying ictogenesis. We performed proteomic analyses to identify proteins with a specific temporal expression pattern in cluster phases and to demonstrate their potential pathomechanistic role. Pilocarpine epilepsy model mice with confirmed cluster pattern of spontaneous recurrent seizures by long-term video-electroencpehalography were sacrificed at the onset, peak, or end of a seizure cluster or in the seizure-free period. Proteomic analysis was performed in the hippocampus and the cortex. Differentially expressed proteins (DEPs) were identified and classified according to their temporal expression pattern. Among the five hippocampal (HC)-DEP classes, HC-class 1 (66 DEPs) represented disrupted cell homeostasis due to clustered seizures, HC-class 2 (63 DEPs) cluster-onset downregulated processes, HC-class 3 (42 DEPs) cluster-onset upregulated processes, and HC-class 4 (103 DEPs) consequences of clustered seizures. Especially, DEPs in HC-class 3 were hippocampus-specific and involved in axonogenesis, synaptic vesicle assembly, and neuronal projection, indicating their pathomechanistic roles in ictogenesis. Key proteins in HC-class 3 were highly interconnected and abundantly involved in those biological processes. This study described the seizure cluster-associated spatiotemporal regulation of protein expression. HC-class 3 provides insights regarding ictogenesis-related processes.Woo-Jin LeeJangsup MoonJung-Ah LimDaejong JeonJung-Suk YooDong-Kyu ParkDohyun HanSoon-Tae LeeKeun-Hwa JungKyung-Il ParkSang Kun LeeKon ChuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Woo-Jin Lee Jangsup Moon Jung-Ah Lim Daejong Jeon Jung-Suk Yoo Dong-Kyu Park Dohyun Han Soon-Tae Lee Keun-Hwa Jung Kyung-Il Park Sang Kun Lee Kon Chu Proteins related to ictogenesis and seizure clustering in chronic epilepsy
description	Abstract Seizure clustering is a common phenomenon in epilepsy. Protein expression profiles during a seizure cluster might reflect the pathomechanism underlying ictogenesis. We performed proteomic analyses to identify proteins with a specific temporal expression pattern in cluster phases and to demonstrate their potential pathomechanistic role. Pilocarpine epilepsy model mice with confirmed cluster pattern of spontaneous recurrent seizures by long-term video-electroencpehalography were sacrificed at the onset, peak, or end of a seizure cluster or in the seizure-free period. Proteomic analysis was performed in the hippocampus and the cortex. Differentially expressed proteins (DEPs) were identified and classified according to their temporal expression pattern. Among the five hippocampal (HC)-DEP classes, HC-class 1 (66 DEPs) represented disrupted cell homeostasis due to clustered seizures, HC-class 2 (63 DEPs) cluster-onset downregulated processes, HC-class 3 (42 DEPs) cluster-onset upregulated processes, and HC-class 4 (103 DEPs) consequences of clustered seizures. Especially, DEPs in HC-class 3 were hippocampus-specific and involved in axonogenesis, synaptic vesicle assembly, and neuronal projection, indicating their pathomechanistic roles in ictogenesis. Key proteins in HC-class 3 were highly interconnected and abundantly involved in those biological processes. This study described the seizure cluster-associated spatiotemporal regulation of protein expression. HC-class 3 provides insights regarding ictogenesis-related processes.
format	article
author	Woo-Jin Lee Jangsup Moon Jung-Ah Lim Daejong Jeon Jung-Suk Yoo Dong-Kyu Park Dohyun Han Soon-Tae Lee Keun-Hwa Jung Kyung-Il Park Sang Kun Lee Kon Chu
author_facet	Woo-Jin Lee Jangsup Moon Jung-Ah Lim Daejong Jeon Jung-Suk Yoo Dong-Kyu Park Dohyun Han Soon-Tae Lee Keun-Hwa Jung Kyung-Il Park Sang Kun Lee Kon Chu
author_sort	Woo-Jin Lee
title	Proteins related to ictogenesis and seizure clustering in chronic epilepsy
title_short	Proteins related to ictogenesis and seizure clustering in chronic epilepsy
title_full	Proteins related to ictogenesis and seizure clustering in chronic epilepsy
title_fullStr	Proteins related to ictogenesis and seizure clustering in chronic epilepsy
title_full_unstemmed	Proteins related to ictogenesis and seizure clustering in chronic epilepsy
title_sort	proteins related to ictogenesis and seizure clustering in chronic epilepsy
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/364c50be800d4201bee1cb4de9140637
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_version_	1718442512924803072

Proteins related to ictogenesis and seizure clustering in chronic epilepsy

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