Revisiting traditional SSR based methodologies available for elephant genetic studies

Abstract Asian elephant (Elephas maximus) plays a significant role in natural ecosystems and it is considered as an endangered animal. Molecular genetics studies on elephants’ dates back to 1990s. Microsatellite markers have been the preferred choice and have played a major role in ecological, evolu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	M. S. L. R. P. Marasinghe, R. M. R. Nilanthi, H. A. B. M. Hathurusinghe, M. G. C. Sooriyabandara, C. H. W. M. R. B. Chandrasekara, K. A. N. C. Jayawardana, M. M. Kodagoda, R. C. Rajapakse, P. C. G. Bandaranayake
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3653038709084926be7d49fd351e5852
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3653038709084926be7d49fd351e5852
record_format	dspace
spelling	oai:doaj.org-article:3653038709084926be7d49fd351e58522021-12-02T13:39:47ZRevisiting traditional SSR based methodologies available for elephant genetic studies10.1038/s41598-021-88034-92045-2322https://doaj.org/article/3653038709084926be7d49fd351e58522021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88034-9https://doaj.org/toc/2045-2322Abstract Asian elephant (Elephas maximus) plays a significant role in natural ecosystems and it is considered as an endangered animal. Molecular genetics studies on elephants’ dates back to 1990s. Microsatellite markers have been the preferred choice and have played a major role in ecological, evolutionary and conservation research on elephants over the past 20 years. However, technical constraints especially related to the specificity of traditionally developed microsatellite markers have brought to question their application, specifically when degraded samples are utilized for analysis. Therefore, we analyzed the specificity of 24 sets of microsatellite markers frequently used for elephant molecular work. Comparative wet lab analysis was done with blood and dung DNA in parallel with in silico work. Our data suggest cross-amplification of unspecific products when field-collected dung samples are utilized in assays. The necessity of Asian elephant specific set of microsatellites and or better molecular techniques are highlighted.M. S. L. R. P. MarasingheR. M. R. NilanthiH. A. B. M. HathurusingheM. G. C. SooriyabandaraC. H. W. M. R. B. ChandrasekaraK. A. N. C. JayawardanaM. M. KodagodaR. C. RajapakseP. C. G. BandaranayakeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q M. S. L. R. P. Marasinghe R. M. R. Nilanthi H. A. B. M. Hathurusinghe M. G. C. Sooriyabandara C. H. W. M. R. B. Chandrasekara K. A. N. C. Jayawardana M. M. Kodagoda R. C. Rajapakse P. C. G. Bandaranayake Revisiting traditional SSR based methodologies available for elephant genetic studies
description	Abstract Asian elephant (Elephas maximus) plays a significant role in natural ecosystems and it is considered as an endangered animal. Molecular genetics studies on elephants’ dates back to 1990s. Microsatellite markers have been the preferred choice and have played a major role in ecological, evolutionary and conservation research on elephants over the past 20 years. However, technical constraints especially related to the specificity of traditionally developed microsatellite markers have brought to question their application, specifically when degraded samples are utilized for analysis. Therefore, we analyzed the specificity of 24 sets of microsatellite markers frequently used for elephant molecular work. Comparative wet lab analysis was done with blood and dung DNA in parallel with in silico work. Our data suggest cross-amplification of unspecific products when field-collected dung samples are utilized in assays. The necessity of Asian elephant specific set of microsatellites and or better molecular techniques are highlighted.
format	article
author	M. S. L. R. P. Marasinghe R. M. R. Nilanthi H. A. B. M. Hathurusinghe M. G. C. Sooriyabandara C. H. W. M. R. B. Chandrasekara K. A. N. C. Jayawardana M. M. Kodagoda R. C. Rajapakse P. C. G. Bandaranayake
author_facet	M. S. L. R. P. Marasinghe R. M. R. Nilanthi H. A. B. M. Hathurusinghe M. G. C. Sooriyabandara C. H. W. M. R. B. Chandrasekara K. A. N. C. Jayawardana M. M. Kodagoda R. C. Rajapakse P. C. G. Bandaranayake
author_sort	M. S. L. R. P. Marasinghe
title	Revisiting traditional SSR based methodologies available for elephant genetic studies
title_short	Revisiting traditional SSR based methodologies available for elephant genetic studies
title_full	Revisiting traditional SSR based methodologies available for elephant genetic studies
title_fullStr	Revisiting traditional SSR based methodologies available for elephant genetic studies
title_full_unstemmed	Revisiting traditional SSR based methodologies available for elephant genetic studies
title_sort	revisiting traditional ssr based methodologies available for elephant genetic studies
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3653038709084926be7d49fd351e5852
work_keys_str_mv	AT mslrpmarasinghe revisitingtraditionalssrbasedmethodologiesavailableforelephantgeneticstudies AT rmrnilanthi revisitingtraditionalssrbasedmethodologiesavailableforelephantgeneticstudies AT habmhathurusinghe revisitingtraditionalssrbasedmethodologiesavailableforelephantgeneticstudies AT mgcsooriyabandara revisitingtraditionalssrbasedmethodologiesavailableforelephantgeneticstudies AT chwmrbchandrasekara revisitingtraditionalssrbasedmethodologiesavailableforelephantgeneticstudies AT kancjayawardana revisitingtraditionalssrbasedmethodologiesavailableforelephantgeneticstudies AT mmkodagoda revisitingtraditionalssrbasedmethodologiesavailableforelephantgeneticstudies AT rcrajapakse revisitingtraditionalssrbasedmethodologiesavailableforelephantgeneticstudies AT pcgbandaranayake revisitingtraditionalssrbasedmethodologiesavailableforelephantgeneticstudies
_version_	1718392608328253440

Revisiting traditional SSR based methodologies available for elephant genetic studies

Ejemplares similares